HEXB B chain Antibody (D-9): sc-376781

HEXB B chain Antibody (D-9) is a mouse monoclonal IgG2b kappa light chain antibody that detects HEXB B chain protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). HEXB B chain Antibody (D-9) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. HEXB B chain protein, also known as beta-hexosaminidase B, is a crucial enzyme that plays a significant role in lysosomal degradation of GM2 gangliosides, which are complex lipids found in the brain. HEXB B chain protein is a tetramer composed of two β-A and two β-B chains, and proper function is essential for maintaining lipid metabolism and preventing accumulation of toxic substances in neuronal cells. Mutations in HEXB gene can lead to Sandhoff disease, a severe autosomal recessive disorder characterized by GM2 ganglioside accumulation, resulting in progressive neurodegeneration. HEXB′s ability to effectively break down GM2 gangliosides is vital for normal brain function, and disruptions in this process can lead to devastating neurological consequences. Anti-HEXB B chain antibody (D-9) enables researchers to gain valuable insights into mechanisms underlying lysosomal storage disorders and explore potential therapeutic avenues for conditions like Sandhoff disease.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

HEXB B chain Antibody (D-9) References:

1. The X-ray crystal structure of human beta-hexosaminidase B provides new insights into Sandhoff disease.  |  Maier, T., et al. 2003. J Mol Biol. 328: 669-81. PMID: 12706724
2. Pathogenic significance of alpha-N-acetylgalactosaminidase activity found in the hemagglutinin of influenza virus.  |  Yamamoto, N. and Urade, M. 2005. Microbes Infect. 7: 674-81. PMID: 15848273
3. N-acetyl-beta-D-hexosaminidase from Trichomonas vaginalis: substrate specificity and activity of inhibitors.  |  Sanon, A., et al. 2005. Biomed Pharmacother. 59: 245-8. PMID: 15890491
4. Beta-hexosaminidase isoenzyme profiles in serum, plasma, platelets and mononuclear, polymorphonuclear and unfractionated total leukocytes.  |  Casal, JA., et al. 2005. Clin Biochem. 38: 938-42. PMID: 16024010
5. Synthesis of a human lysosomal enzyme, beta-hexosaminidase B, using the baculovirus expression system.  |  Boose, JA., et al. 1990. Protein Expr Purif. 1: 111-20. PMID: 1967020
6. Introduction of the alpha subunit mutation associated with the B1 variant of Tay-Sachs disease into the beta subunit produces a beta-hexosaminidase B without catalytic activity.  |  Brown, CA., et al. 1989. J Biol Chem. 264: 21705-10. PMID: 2532211
7. Isolation of cDNA clones coding for the beta subunit of human beta-hexosaminidase.  |  O'Dowd, BF., et al. 1985. Proc Natl Acad Sci U S A. 82: 1184-8. PMID: 2579389
8. Protease-resistant modified human β-hexosaminidase B ameliorates symptoms in GM2 gangliosidosis model.  |  Kitakaze, K., et al. 2016. J Clin Invest. 126: 1691-703. PMID: 27018595
9. Demonstration of a Sandhoff disease-associated autosomal 50-kb deletion by field inversion gel electrophoresis.  |  Bikker, H., et al. 1989. Hum Genet. 81: 287-8. PMID: 2921040
10. Oxidative stress induces extracellular vesicle release by upregulation of HEXB to facilitate tumour growth in experimental hepatocellular carcinoma.  |  Duan, J., et al. 2024. J Extracell Vesicles. 13: e12468. PMID: 38944674
11. Hexosaminidase isozyme in type O Gm2 gangliosidosis (Sandhoff-Jatzkewitz disease).  |  Beutler, E., et al. 1975. Am J Hum Genet. 27: 628-38. PMID: 808963
12. Evidence for the involvement of Glu-355 in the catalytic action of human beta-hexosaminidase B.  |  Pennybacker, M., et al. 1997. J Biol Chem. 272: 8002-6. PMID: 9065471

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-HEXB B chain Antibody (D-9) has 10 citations in a variety of scientific publications.

Primary antibodies like Anti-HEXB B chain Antibody (D-9) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

HEXB B chain Antibody (D-9) Specifications

Alternate Product Name: HEXB B chain

Product Clone Name: D-9

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 122-166

Epitope Target: HEXB

Epitope Species: human

Product Reactivity/Detection: HEXB B chain

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 10 citations in various scientific publications

Available Conjugates/Forms for HEXB B chain Antibody (D-9): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin