HES6 Antibody (F-5): sc-133196

HES6 Antibody (F-5) is a mouse monoclonal IgG1 kappa light chain antibody that detects HES6 of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). Anti-HES6 antibody (F-5) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. HES6 plays a crucial role in regulating transcription during embryonic development, particularly in the Notch signaling pathway, which is essential for proper segmentation and neural development. Unlike other members of the HES gene family, HES6 does not bind directly to DNA; instead, HES6 functions as a negative regulator of HES1, thereby promoting transcriptional activity. This unique mechanism highlights HES6′s importance in balancing transcriptional outputs of the HES family, which is vital for developing various tissues, including brain and limb buds in embryos. HES6 expression in these regions underscores HES6′s significance in developmental processes, making HES6 a valuable target for research in developmental biology and related fields.

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HES6 Antibody (F-5) References:

1. The bHLH gene Hes6, an inhibitor of Hes1, promotes neuronal differentiation.  |  Bae, S., et al. 2000. Development. 127: 2933-43. PMID: 10851137
2. Two mammalian helix-loop-helix factors structurally related to Drosophila hairy and Enhancer of split.  |  Sasai, Y., et al. 1992. Genes Dev. 6: 2620-34. PMID: 1340473
3. Molecular characterization of a rat negative regulator with a basic helix-loop-helix structure predominantly expressed in the developing nervous system.  |  Akazawa, C., et al. 1992. J Biol Chem. 267: 21879-85. PMID: 1400497
4. Structure, chromosomal locus, and promoter analysis of the gene encoding the mouse helix-loop-helix factor HES-1. Negative autoregulation through the multiple N box elements.  |  Takebayashi, K., et al. 1994. J Biol Chem. 269: 5150-6. PMID: 7906273
5. Molecular characterization of HES-2, a mammalian helix-loop-helix factor structurally related to Drosophila hairy and Enhancer of split.  |  Ishibashi, M., et al. 1993. Eur J Biochem. 215: 645-52. PMID: 8354270
6. The WRPW motif of the hairy-related basic helix-loop-helix repressor proteins acts as a 4-amino-acid transcription repression and protein-protein interaction domain.  |  Fisher, AL., et al. 1996. Mol Cell Biol. 16: 2670-7. PMID: 8649374
7. Molecular interaction between TLE1 and the carboxyl-terminal domain of HES-1 containing the WRPW motif.  |  Grbavec, D. and Stifani, S. 1996. Biochem Biophys Res Commun. 223: 701-5. PMID: 8687460
8. Expression of the helix-loop-helix factor, Hes3, during embryo development suggests a role in early midbrain-hindbrain patterning.  |  Lobe, CG. 1997. Mech Dev. 62: 227-37. PMID: 9152013

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-HES6 Antibody (F-5) has 6 citations in a variety of scientific publications.

Primary antibodies like Anti-HES6 Antibody (F-5) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

HES6 Antibody (F-5) Specifications

Alternate Product Name: HES6

Product Clone Name: F-5

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 45-224

Epitope Target: HES6

Epitope Species: human

Product Reactivity/Detection: HES6

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 6 citations in various scientific publications

Available Conjugates/Forms for HES6 Antibody (F-5): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin, X: TransCruz Reagent