HES1 Antibody (E-5): sc-166410

HES1 Antibody (E-5) is a mouse monoclonal IgG2b kappa light chain antibody that detects HES1 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). HES1 (E-5) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. HES1 plays a crucial role in the regulation of myogenesis and neurogenesis, functioning as a transcriptional repressor within the Notch signaling pathway. This pathway is essential for proper cell differentiation and development, particularly during embryogenesis, where anti-HES1 antibody (E-5) helps to control segmentation and neural development. The HES gene family, which includes HES1, is homologous to the Drosophila Hairy and enhancer of split genes and encodes basic helix-loop-helix transcription factors that interact with the TLE proteins, the mammalian counterparts of groucho. This interaction is mediated by the carboxy-terminal WRPW motif of the HES proteins, allowing the HES/TLE complex to bind directly to DNA and regulate gene expression by repressing activator proteins. Notably, HES1 and HES5 preferentially bind to the N box (CACNAG) rather than the E box (CANNTG), highlighting their specific regulatory roles in various tissues. HES1 and HES2 are expressed in a range of adult and embryonic tissues, underscoring their importance in developmental processes and potential implications in various diseases.

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HES1 Antibody (E-5) References:

1. The bHLH gene Hes6, an inhibitor of Hes1, promotes neuronal differentiation.  |  Bae, S., et al. 2000. Development. 127: 2933-43. PMID: 10851137
2. A Hes1-based oscillator in cultured cells and its potential implications for the segmentation clock.  |  Dale, JK. and Maroto, M. 2003. Bioessays. 25: 200-3. PMID: 12596223
3. Two mammalian helix-loop-helix factors structurally related to Drosophila hairy and Enhancer of split.  |  Sasai, Y., et al. 1992. Genes Dev. 6: 2620-34. PMID: 1340473
4. Molecular characterization of a rat negative regulator with a basic helix-loop-helix structure predominantly expressed in the developing nervous system.  |  Akazawa, C., et al. 1992. J Biol Chem. 267: 21879-85. PMID: 1400497
5. Hes1 plays an essential role in Kras-driven pancreatic tumorigenesis.  |  Nishikawa, Y., et al. 2019. Oncogene. 38: 4283-4296. PMID: 30705405
6. Hes1 attenuates type I IFN responses via VEGF-C and WDFY1.  |  Ning, F., et al. 2019. J Exp Med. 216: 1396-1410. PMID: 31015298
7. Dysregulation of SIRT3 SUMOylation Confers AML Chemoresistance via Controlling HES1-Dependent Fatty Acid Oxidation.  |  Zhang, Y., et al. 2022. Int J Mol Sci. 23: PMID: 35955415
8. Structure, chromosomal locus, and promoter analysis of the gene encoding the mouse helix-loop-helix factor HES-1. Negative autoregulation through the multiple N box elements.  |  Takebayashi, K., et al. 1994. J Biol Chem. 269: 5150-6. PMID: 7906273
9. Molecular characterization of HES-2, a mammalian helix-loop-helix factor structurally related to Drosophila hairy and Enhancer of split.  |  Ishibashi, M., et al. 1993. Eur J Biochem. 215: 645-52. PMID: 8354270
10. The WRPW motif of the hairy-related basic helix-loop-helix repressor proteins acts as a 4-amino-acid transcription repression and protein-protein interaction domain.  |  Fisher, AL., et al. 1996. Mol Cell Biol. 16: 2670-7. PMID: 8649374
11. Molecular interaction between TLE1 and the carboxyl-terminal domain of HES-1 containing the WRPW motif.  |  Grbavec, D. and Stifani, S. 1996. Biochem Biophys Res Commun. 223: 701-5. PMID: 8687460
12. Expression of the helix-loop-helix factor, Hes3, during embryo development suggests a role in early midbrain-hindbrain patterning.  |  Lobe, CG. 1997. Mech Dev. 62: 227-37. PMID: 9152013

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-HES1 Antibody (E-5) has 54 citations in a variety of scientific publications.

Primary antibodies like Anti-HES1 Antibody (E-5) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

HES1 Antibody (E-5) Specifications

Alternate Product Name: HES1

Product Clone Name: E-5

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 237-261

Epitope Target: HES1

Epitope Species: human

Product Reactivity/Detection: HES1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: bovine and porcine

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 54 citations in various scientific publications

Available Conjugates/Forms for HES1 Antibody (E-5): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin, X: TransCruz Reagent