Anti-HES1 Antibody (E-5) recommended for detection of HES1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

Anti-HES1 Antibody (E-5): sc-166410

Anti-HES1 Antibody (E-5) is rated 4.3 out of 5 by 16.
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Datasheets

    • Anti-HES1 Antibody (E-5) is a mouse monoclonal IgG2b κ HES1 antibody, cited in 41 publications, provided at 200 µg/ml
    • specific for an epitope mapping between amino acids 237-261 near the C-terminus of HES1 of human origin
    • Anti-HES1 Antibody (E-5) is recommended for detection of HES1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including and bovine and porcine
    • Anti-HES1 Antibody (E-5) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • TransCruz reagent for Gel Supershift and ChIP applications (sc-166410 X, 200 µg/0.1 ml)
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of HES1 (E-5): sc-166410.
    • m-IgG2b BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for HES1 Antibody (E-5) for WB and IHC(P) applications. These reagents are now offered in bundles with HES1 Antibody (E-5) (see ordering information below).
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Can the blocking peptide sc-166410 P be used in Western Blot as a positive control?

Asked by: AbPolly
Thank you for your question. The blocking peptide sc-166410 P is a short synthetic peptide and is intended for use as a negative control by pre-adsorption and is not appropriate for use as a positive control is Western Blot. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support 2
Date published: 2017-01-17
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Rated 4 out of 5 by from Great antibody, clear bands in western blot. I received a sample of this antibody (10ug/50ul). I tested it on protein extracts (30ug) of a cell line of trophoblasts induced to differentiate to an endothelial-like phenotype. First antibody dilution: 1: 1000 (0.2 ug/ml) Dilution second antibody (anti mouse - HRP): 1: 5000 Detection: ECL Exposure time: 45 sec. Approx.
Date published: 2018-05-14
Rated 4 out of 5 by from GOOD ANTIBODY I got this antibody as a sample and it is working good.
Date published: 2018-03-29
Rated 2 out of 5 by from no good signal in IF I didn't detect good staining signal in 4%PFA fixed embryonic mouse brain(after antigen retrieval)
Date published: 2018-03-07
Rated 5 out of 5 by from Works on colon tissue sections I used Hes1 antibody on my colon tissue sections and it worked very nice.
Date published: 2017-10-10
Rated 5 out of 5 by from Very good Antibody sample, 1:200 dilution for immunostaining of paraffin-embedded sections from a xenograft model of oral cancer, which showing cytoplasmic and nuclear staining of cells.
Date published: 2017-08-18
Rated 3 out of 5 by from can't recognize any band in NPC cell lines tried twice, still can't see any band in NPC cell lines
Date published: 2017-07-12
Rated 5 out of 5 by from Great features I used the antibody to detect the hes1 expression in mouse embryonic tissue. After I incubate the membrane for 2 days in antibody dilution buffer, I got very brilliant results.
Date published: 2017-06-22
Rated 5 out of 5 by from great It is a very good antibody, It was very stable and the result could be repeated
Date published: 2017-05-11
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