Anti-HES1 Antibody (E-5) is a mouse monoclonal IgG2b (kappa light chain) HES1 antibody provided at 200 µg/ml
specific for an epitope mapping between amino acids 237-261 near the C-terminus of HES1 of human origin
Anti-HES1 Antibody (E-5) is recommended for detection of HES1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including and bovine and porcine
Anti-HES1 Antibody (E-5) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
TransCruz reagent for Gel Supershift and ChIP applications (sc-166410 X, 200 µg/0.1 ml)
blocking peptide, sc-166410 P
See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of HES1 (E-5): sc-166410.
Every item is shipped based on the best shipping method assessed for the temperature requirements of that specific item. Items are grouped and shipped together whenever
possible, and a separate shipping charge will be included for each shipping method required. Shipping charges listed below are from our US warehouses to the Contiguous US,
Alaska, Hawaii, Canada and Puerto Rico. Shipping charges for countries outside the US and Canada will be determined once order has been received
Please note: We can not ship to PO boxes
Express Blue Ice
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Animal Health Prescription Item
SHIPPING METHODS & CHARGES
Ships via FedEx Ground to Contiguous US, Alaska, Canada, Monday through Friday. This method is used for less temperature sensitive items such as lab ware and animal
health products, bulky and/or heavy items
Labware ships FedEx Ground free of charge to the contiguous US
HES1 Antibody (E-5) is a high quality monoclonal HES1 antibody suitable for the detection of the HES1 protein of mouse, rat and human origin. HES1 Antibody (E-5) is available as both the non-conjugated anti-HES1 antibody form, as well as multiple conjugated forms of anti-HES1 antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. The Drosophila Hairy and enhancer of split genes encode basic helix-loop-helix (bHLH) transcriptional repressors that function in the Notch signaling pathway and control segmentation and neural development during embryogenesis. The mammalian homolog of Drosophila Hairy and enhancer of split are the HES gene family members HES1-6, which also encode bHLH transcriptional repressors that regulate myogenesis and neurogenesis. The HES family members form a complex with TLE, the mammalian homolog of groucho, and this interaction is mediated by the carboxy-terminal WRPW motif of the HES proteins. The HES/TLE complex functions by directly binding to DNA instead of interfering with activator proteins. Most HES family members, including HES1 and HES5, preferentially bind to the N box (CACNAG) as opposed to the E box (CANNTG). HES1 and HES2 are expressed in a variety of adult and embryonic tissues.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Can the blocking peptide sc-166410 P be used in Western Blot as a positive control?
Asked by: AbPolly
Thank you for your question. The blocking peptide sc-166410 P is a short synthetic peptide and is intended for use as a negative control by pre-adsorption and is not appropriate for use as a positive control is Western Blot. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Rated 4 out of
Great antibody, clear bands in western blot.I received a sample of this antibody (10ug/50ul). I tested it on protein extracts (30ug) of a cell line of trophoblasts induced to differentiate to an endothelial-like phenotype. First antibody dilution: 1: 1000 (0.2 ug/ml) Dilution second antibody (anti mouse - HRP): 1: 5000 Detection: ECL Exposure time: 45 sec. Approx.
Date published: 2018-05-14
Rated 4 out of
GOOD ANTIBODYI got this antibody as a sample and it is working good.
Date published: 2018-03-29
Rated 2 out of
no good signal in IFI didn't detect good staining signal in 4%PFA fixed embryonic mouse brain(after antigen retrieval)
Date published: 2018-03-07
Rated 5 out of
Ahmed I from
Works on colon tissue sectionsI used Hes1 antibody on my colon tissue sections and it worked very nice.
Date published: 2017-10-10
Rated 5 out of
Very goodAntibody sample, 1:200 dilution for immunostaining of paraffin-embedded sections from a xenograft model of oral cancer, which showing cytoplasmic and nuclear staining of cells.
Date published: 2017-08-18
Rated 3 out of
can't recognize any band in NPC cell linestried twice, still can't see any band in NPC cell lines
Date published: 2017-07-12
Rated 5 out of
Shasta Alice from
Great featuresI used the antibody to detect the hes1 expression in mouse embryonic tissue. After I incubate the membrane for 2 days in antibody dilution buffer, I got very brilliant results.
Date published: 2017-06-22
Rated 5 out of
greatIt is a very good antibody, It was very stable and the result could be repeated
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