HES1 Antibody (A-12) recommended for detection of HES1 of mouse, rat and human origin by WB, IP, IF and ELISA

HES1 Antibody (A-12): sc-166378

HES1 Antibody (A-12) is rated 4.0 out of 5 by 4.
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Datasheets

    • HES1 Antibody (A-12) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • raised against amino acids 163-194 of HES1 of human origin
    • Anti-HES1 Antibody (A-12) is recommended for detection of HES1 of mouse, rat and human origin by WB, IP, IF and ELISA
    • Anti-HES1 Antibody (A-12) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • TransCruz reagent for ChIP application (sc-166378 X, 200 µg/0.1 ml)
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of HES1 (A-12): sc-166378.
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I need to use an antibody to detect the protein in samples of bovine origin. Do you know if this mouse monoclonal antibody will work?

Asked by: jerojero
Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity.
Answered by: Technical Support 2
Date published: 2017-02-08
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Rated 1 out of 5 by from HES1 Ab We used 1:500 dilution Still no signal. Cell line used is Melan-a cells
Date published: 2017-11-06
Rated 5 out of 5 by from Published in WB and IHC This mouse monoclonal, HES1 Antibody (A-12), has been featured in the literature for applications including Western blot and IHC/IF.
Date published: 2017-01-26
Rated 5 out of 5 by from Great Western Blot data of HES1 expression in non Great Western Blot data of HES1 expression in non-transfected and human HES1 transfected 293T whole cell lysates. -SCBT QC
Date published: 2015-02-18
Rated 5 out of 5 by from Good Western Blot data of HES1 expression in non Good Western Blot data of HES1 expression in non-transfected and mouse HES1 transfected 293T whole cell lysates. -SCBT QC
Date published: 2013-09-01
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