HERG Antibody (F-12): sc-377388

HERG Antibody (F-12) is a mouse monoclonal IgG1 kappa light chain antibody that detects HERG protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-HERG antibody (F-12) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. The human ether-a-go-go related gene (HERG) encodes the pore-forming α subunit of the delayed rectifier potassium channel I_Kr, which plays a crucial role in cardiac repolarization. HERG protein is characterized by six transmembrane α-helices and a reentrant "pore-loop" that is essential for ion transport function. Notably, two N-terminal splice variants of HERG, isoform 1α and isoform 1β, exhibit different functional properties; isoform 1β, which lacks the PAS motif, deactivates more rapidly than isoform 1α, influencing channel kinetics. HERG expression is predominantly found in the heart, particularly in ventricles, where HERG is vital for maintaining electrical stability of cardiac cells. Mutations in HERG can lead to significant cardiac arrhythmias, including inherited long QT syndrome, by increasing beat-to-beat variability and early after depolarization, which can trigger premature heartbeats. Understanding HERG function and regulation is essential for elucidating mechanisms underlying cardiac health and disease.

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HERG Antibody (F-12) References:

1. Expression of distinct ERG proteins in rat, mouse, and human heart. Relation to functional I(Kr) channels.  |  Pond, AL., et al. 2000. J Biol Chem. 275: 5997-6006. PMID: 10681594
2. Distinct gene-specific mechanisms of arrhythmia revealed by cardiac gene transfer of two long QT disease genes, HERG and KCNE1.  |  Hoppe, UC., et al. 2001. Proc Natl Acad Sci U S A. 98: 5335-40. PMID: 11320260
3. Functional characterization of the C-terminus of the human ether-à-go-go-related gene K(+) channel (HERG).  |  Aydar, E. and Palmer, C. 2001. J Physiol. 534: 1-14. PMID: 11432987
4. Activation of human ether-a-go-go related gene (hERG) potassium channels by small molecules.  |  Zhou, PZ., et al. 2011. Acta Pharmacol Sin. 32: 781-8. PMID: 21623390
5. The Human Ether-a-go-go-related Gene (hERG) Potassium Channel Represents an Unusual Target for Protease-mediated Damage.  |  Lamothe, SM., et al. 2016. J Biol Chem. 291: 20387-401. PMID: 27502273
6. Human ether‑à‑go‑go‑related gene mutation L539fs/47‑hERG leads to cell apoptosis through the endoplasmic reticulum stress pathway.  |  Ma, S., et al. 2019. Int J Mol Med. 43: 1253-1262. PMID: 30628647
7. Inhibition of Human Ether-A-Go-Go-Related Gene (hERG) Potassium Current by the Novel Sotalol Analogue, Soestalol.  |  Shugg, T., et al. 2020. JACC Clin Electrophysiol. 6: 756-759. PMID: 32703555
8. A mechanistic link between an inherited and an acquired cardiac arrhythmia: HERG encodes the IKr potassium channel.  |  Sanguinetti, MC., et al. 1995. Cell. 81: 299-307. PMID: 7736582
9. A molecular basis for cardiac arrhythmia: HERG mutations cause long QT syndrome.  |  Curran, ME., et al. 1995. Cell. 80: 795-803. PMID: 7889573
10. Mutations in the K+ channel signature sequence.  |  Heginbotham, L., et al. 1994. Biophys J. 66: 1061-7. PMID: 8038378
11. Electrophysiological characterization of an alternatively processed ERG K+ channel in mouse and human hearts.  |  Lees-Miller, JP., et al. 1997. Circ Res. 81: 719-26. PMID: 9351446
12. The structure of the potassium channel: molecular basis of K+ conduction and selectivity.  |  Doyle, DA., et al. 1998. Science. 280: 69-77. PMID: 9525859

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-HERG Antibody (F-12) has 10 citations in a variety of scientific publications.

Primary antibodies like Anti-HERG Antibody (F-12) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

HERG Antibody (F-12) Specifications

Alternate Product Name: KCNH2

Product Clone Name: F-12

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 96-270

Epitope Target: HERG

Epitope Species: human

Product Reactivity/Detection: HERG

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 10 citations in various scientific publications

Available Conjugates/Forms for HERG Antibody (F-12): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin