Herc2 Antibody (A-5): sc-515891
- Herc2 Antibody (A-5) is a mouse monoclonal IgG2b κ Herc2 antibody provided at 200 µg/ml
- raised against amino acids 1-300 mapping at the N-terminus of Herc2 of human origin
- Herc2 Antibody (A-5) is recommended for detection of Herc2 of human origin by WB, IP, IF and ELISA
- Anti-Herc2 Antibody (A-5) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG2b BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for Herc2 Antibody (A-5) for WB applications. These reagents are now offered in bundles with Herc2 Antibody (A-5) (see ordering information below).
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Herc2 Antibody (A-5) is a mouse monoclonal IgG2b kappa light chain antibody that detects Herc2 of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). anti-Herc2 antibody (A-5) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Herc2, also known as hect domain and RLD 2, jdf2, p528, or SHEP1, is a large protein consisting of 4,834 amino acids and features several functional domains such as WD repeats, RCC1 repeats, HECT domains, and ZZ-type zinc fingers. Herc2 plays a crucial role in the ubiquitin-proteasome pathway, functioning primarily as an E3 ubiquitin-protein ligase. Herc2 facilitates the transfer of ubiquitin from an E2 ubiquitin-conjugating enzyme to specific substrate proteins, marking them for degradation. This process is vital for maintaining cellular homeostasis by regulating protein levels and removing damaged or misfolded proteins. Additionally, variations in the Herc2 gene have been linked to skin, hair, and eye pigmentation variability, highlighting its significance in phenotypic diversity.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Herc2 Antibody (A-5) References:
- Structure of the highly conserved HERC2 gene and of multiple partially duplicated paralogs in human. | Ji, Y., et al. 2000. Genome Res. 10: 319-29. PMID: 10720573
- Blue eye color in humans may be caused by a perfectly associated founder mutation in a regulatory element located within the HERC2 gene inhibiting OCA2 expression. | Eiberg, H., et al. 2008. Hum Genet. 123: 177-87. PMID: 18172690
- Three genome-wide association studies and a linkage analysis identify HERC2 as a human iris color gene. | Kayser, M., et al. 2008. Am J Hum Genet. 82: 411-23. PMID: 18252221
- A single SNP in an evolutionary conserved region within intron 86 of the HERC2 gene determines human blue-brown eye color. | Sturm, RA., et al. 2008. Am J Hum Genet. 82: 424-31. PMID: 18252222
- A genome-wide association study identifies novel alleles associated with hair color and skin pigmentation. | Han, J., et al. 2008. PLoS Genet. 4: e1000074. PMID: 18483556
- Linkage and association analysis of spectrophotometrically quantified hair color in Australian adolescents: the effect of OCA2 and HERC2. | Shekar, SN., et al. 2008. J Invest Dermatol. 128: 2807-14. PMID: 18528436
- Interactions between HERC2, OCA2 and MC1R may influence human pigmentation phenotype. | Branicki, W., et al. 2009. Ann Hum Genet. 73: 160-70. PMID: 19208107
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Herc2 Antibody (A-5) | sc-515891 | 200 µg/ml | $322.00 | |||
Herc2 Antibody (A-5): m-IgGκ BP-HRP Bundle | sc-525270 | 200 µg Ab, 40 µg BP | $361.00 | |||
Herc2 Antibody (A-5): m-IgG2b BP-HRP Bundle | sc-549223 | 200 µg Ab; 10 µg BP | $361.00 | |||
Herc2 Antibody (A-5) AC | sc-515891 AC | 500 µg/ml, 25% agarose | $424.00 | |||
Herc2 Antibody (A-5) HRP | sc-515891 HRP | 200 µg/ml | $322.00 | |||
Herc2 Antibody (A-5) FITC | sc-515891 FITC | 200 µg/ml | $336.00 | |||
Herc2 Antibody (A-5) PE | sc-515891 PE | 200 µg/ml | $349.00 | |||
Herc2 Antibody (A-5) Alexa Fluor® 488 | sc-515891 AF488 | 200 µg/ml | $364.00 | |||
Herc2 Antibody (A-5) Alexa Fluor® 546 | sc-515891 AF546 | 200 µg/ml | $364.00 | |||
Herc2 Antibody (A-5) Alexa Fluor® 594 | sc-515891 AF594 | 200 µg/ml | $364.00 | |||
Herc2 Antibody (A-5) Alexa Fluor® 647 | sc-515891 AF647 | 200 µg/ml | $364.00 | |||
Herc2 Antibody (A-5) Alexa Fluor® 680 | sc-515891 AF680 | 200 µg/ml | $364.00 | |||
Herc2 Antibody (A-5) Alexa Fluor® 790 | sc-515891 AF790 | 200 µg/ml | $364.00 |