Herc2 Activators
Herc2 Activators are a curated set of chemical compounds that enhance the functional activity of Herc2 through various cellular mechanisms. PhHerc2 Activators encompass a diverse array of chemical compounds that indirectly enhance the E3 ubiquitin ligase activity of Herc2, a pivotal enzyme in protein quality control and DNA repair pathways. Compounds like Phosphatidylinositol 4,5-bisphosphate (PIP2) and Uridine Triphosphate (UTP) augment signaling through the PI3K/Akt pathway, which is closely associated with protein trafficking and cellular stress responses, processes Herc2 is directly involved in. The heightened activity of this pathway could enhance the substrate recognition and ubiquitination efficiency of Herc2. Similarly, agents like Forskolin and Caffeine elevate intracellular cAMP, subsequently activating PKA which may further phosphorylate proteins to modulate Herc2's interactions with its substrates, thereby enhancing its ligase activity. Additionally, Ionomycin's elevation of intracellular calcium levels activates calcium-dependent kinases which could influence Herc2's function, while Okadaic Acid's inhibition of protein phosphatases leads to a hyperphosphorylated proteome, potentially increasing Herc2's ubiquitination targets.
Zinc Pyrithione's modulation of metal ion homeostasis may enhance Herc2's activity by affecting its structural conformation or interaction with substrates. The Hsp90 inhibitor 17-AAG destabilizes client proteins, intensifying the cellular demand for Herc2's role in tagging misfolded proteins for degradation. Lastly, Sodium Orthovanadate serves as a phosphatase inhibitor, ensuring a surplus of phosphorylated proteins, which are potential substrates for Herc2's ubiquitin ligase activity. Through these multifaceted biochemical pathways, Herc2 Activators collectively enhance the enzymatic action of Herc2 without upregulating its expression or directly altering its intrinsic activity.
SEE ALSO...
| Product Name | CAS # | Catalog # | QUANTITY | Price | Citations | RATING |
|---|---|---|---|---|---|---|
NAD+, Free Acid | 53-84-9 | sc-208084B sc-208084 sc-208084A sc-208084C sc-208084D sc-208084E sc-208084F | 1 g 5 g 10 g 25 g 100 g 1 kg 5 kg | $57.00 $191.00 $302.00 $450.00 $1800.00 $3570.00 $10710.00 | 4 | |
NAD+ is a substrate for sirtuins, which are known to modify proteins and influence their activity. Sirtuin-mediated deacetylation has been shown to impact various proteins involved in DNA repair and ubiquitination, potentially enhancing Herc2's role in these processes. | ||||||
Forskolin | 66575-29-9 | sc-3562 sc-3562A sc-3562B sc-3562C sc-3562D | 5 mg 50 mg 1 g 2 g 5 g | $78.00 $153.00 $740.00 $1413.00 $2091.00 | 73 | |
Forskolin increases intracellular cAMP levels, leading to the activation of PKA. PKA can phosphorylate proteins that interact with Herc2, potentially enhancing its ubiquitination activity by changing the conformation or accessibility of its substrates. | ||||||
Ionomycin | 56092-82-1 | sc-3592 sc-3592A | 1 mg 5 mg | $78.00 $270.00 | 80 | |
Ionomycin is a calcium ionophore that increases intracellular calcium levels, which can activate calcium/calmodulin-dependent protein kinases. These kinases can modify proteins that associate with Herc2, potentially augmenting Herc2's ligase activity. | ||||||
Okadaic Acid | 78111-17-8 | sc-3513 sc-3513A sc-3513B | 25 µg 100 µg 1 mg | $291.00 $530.00 $1800.00 | 78 | |
Okadaic Acid is a potent inhibitor of protein phosphatases 1 and 2A, leading to increased phosphorylation levels of various proteins. This hyperphosphorylated state could enhance Herc2's interaction with phosphorylated substrates, thus enhancing its E3 ubiquitin ligase activity. | ||||||
Zinc | 7440-66-6 | sc-213177 | 100 g | $48.00 | ||
1-Hydroxypyridine-2-thione zinc salt can modulate metal ion homeostasis within cells. Zinc ions are critical cofactors for many proteins, including those involved in the ubiquitin-proteasome system. Enhanced zinc levels might indirectly increase Herc2's catalytic activity. | ||||||
Uridine-5′-triphosphate, Trisodium Salt | 19817-92-6 | sc-301964 sc-301964A | 50 mg 1 g | $88.00 $120.00 | 2 | |
UTP, through its role as an agonist of P2Y receptors, can lead to the activation of downstream signaling pathways like PI3K/Akt, which could indirectly enhance Herc2's activity by affecting its substrate interactions or stability. | ||||||
17-AAG | 75747-14-7 | sc-200641 sc-200641A | 1 mg 5 mg | $67.00 $156.00 | 16 | |
17-AAG is an Hsp90 inhibitor that can lead to the destabilization of client proteins, subsequently increasing the need for cellular protein quality control mechanisms, where Herc2 plays a significant role as an E3 ubiquitin ligase. | ||||||
Caffeine | 58-08-2 | sc-202514 sc-202514A sc-202514B sc-202514C sc-202514D | 50 g 100 g 250 g 1 kg 5 kg | $33.00 $67.00 $97.00 $192.00 $775.00 | 13 | |
Caffeine is a known inhibitor of phosphodiesterases, leading to increased cAMP levels, and can also affect the ATM/ATR pathways, which are involved in DNA damage response. Herc2 is implicated in DNA repair processes, and modulating these pathways could enhance its activity. | ||||||
Sodium Orthovanadate | 13721-39-6 | sc-3540 sc-3540B sc-3540A | 5 g 10 g 50 g | $49.00 $57.00 $187.00 | 142 | |
Sodium Orthovanadate is a phosphatase inhibitor that can lead to enhanced phosphorylation of proteins. This can potentiate the ubiquitination activity of Herc2 by increasing the number of phosphorylated substrates available for recognition and ubiquitination. | ||||||