HePTP Antibody (E-5): sc-271245

HePTP Antibody (E-5) is a mouse monoclonal IgG1 kappa light chain antibody that detects HePTP protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). anti-HePTP antibody (E-5) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Hematopoietic protein tyrosine phosphatase (HePTP) plays a crucial role in regulating cellular signaling pathways, particularly in T cells, where HePTP modulates the activation of the T cell receptor (TCR) and downstream mitogen-activated protein kinase (MAPK) pathways. This regulation is vital for maintaining proper immune responses, as overexpression of HePTP can inhibit TCR-induced activation of ERK 2, thereby influencing T cell proliferation and differentiation. The gene encoding HePTP, located on chromosome 1q32.1, is frequently mutated in preleukemic myeloproliferative diseases, highlighting HePTP′s potential involvement in oncogenic processes. HePTP is predominantly expressed in the thymus, spleen, and various leukemic cell lines, including Jurkat T leukemia cells, and HePTP expression is transcriptionally activated in T cells upon stimulation with cytokines such as Interleukin 6. Furthermore, HePTP mRNA levels significantly increase in normal mouse lymphocytes when stimulated with agents like phytohemagglutinin, lipopolysaccharide, concanavalin A, and anti-CD3, underscoring HePTP′s importance in immune cell activation and function.

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HePTP Antibody (E-5) References:

1. Inhibition of T cell signaling by mitogen-activated protein kinase-targeted hematopoietic tyrosine phosphatase (HePTP).  |  Saxena, M., et al. 1999. J Biol Chem. 274: 11693-700. PMID: 10206983
2. Hematopoietic protein tyrosine phosphatase suppresses extracellular stimulus-regulated kinase activation.  |  Gronda, M., et al. 2001. Mol Cell Biol. 21: 6851-8. PMID: 11564869
3. Cloning and expression of an inducible lymphoid-specific, protein tyrosine phosphatase (HePTPase).  |  Zanke, B., et al. 1992. Eur J Immunol. 22: 235-9. PMID: 1530918
4. Induction of protein-tyrosine phosphatase LC-PTP by IL-2 in human T cells. LC-PTP is an early response gene.  |  Adachi, M., et al. 1994. FEBS Lett. 338: 47-52. PMID: 8307155
5. A hematopoietic protein tyrosine phosphatase (HePTP) gene that is amplified and overexpressed in myeloid malignancies maps to chromosome 1q32.1.  |  Zanke, B., et al. 1994. Leukemia. 8: 236-44. PMID: 8309248
6. Negative regulation of T cell antigen receptor signal transduction by hematopoietic tyrosine phosphatase (HePTP).  |  Saxena, M., et al. 1998. J Biol Chem. 273: 15340-4. PMID: 9624114

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-HePTP Antibody (E-5) has 1 citations in a variety of scientific publications.

Primary antibodies like Anti-HePTP Antibody (E-5) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

HePTP Antibody (E-5) Specifications

Alternate Product Name: HePTP

Product Clone Name: E-5

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 46-125

Epitope Target: HePTP

Epitope Species: human

Product Reactivity/Detection: HePTP

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 1 citations in various scientific publications

Available Conjugates/Forms for HePTP Antibody (E-5): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin