hepatic OAT Antibody (D-10): sc-376050
- hepatic OAT Antibody (D-10) is a mouse monoclonal IgG1 κ hepatic OAT antibody provided at 200 µg/ml
- raised against amino acids 101-230 mapping within an internal region of OAT of human origin
- hepatic OAT Antibody (D-10) is recommended for detection of hepatic OAT of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-hepatic OAT Antibody (D-10) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for hepatic OAT Antibody (D-10) for WB applications. These reagents are now offered in bundles with hepatic OAT Antibody (D-10) (see ordering information below).
QUICK LINKS
Hepatic OAT Antibody (D-10) is a mouse monoclonal IgG1 kappa light chain antibody that detects hepatic OAT protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Hepatic OAT (D-10) antibody is available in non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. OAT protein, or ornithine aminotransferase, is a crucial enzyme composed of 439 amino acids and encoded by the human gene OAT. OAT plays a vital role in the urea cycle, primarily located in the mitochondrial matrix, where OAT functions as a homotetramer. This enzyme catalyzes the conversion of ornithine and α-ketoglutarate to glutamate and proline, a reaction essential for amino acid metabolism and nitrogen balance in the body. Deficiencies in OAT can lead to ornithinemia, a condition associated with gyrate atrophy of the choroid and retina, which manifests as progressive vision loss starting with night blindness. The hepatic form of OAT is generated by the cleavage of a 25 amino acid transit peptide from the N-terminus of the precursor protein, while the renal variant arises from the removal of a 35 amino acid transit peptide. Understanding the function and importance of hepatic OAT is crucial for elucidating metabolic disorders and developing potential therapeutic strategies.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
hepatic OAT Antibody (D-10) References:
- Somatic versus germline mutation processes at minisatellite CEB1 (D2S90) in humans and transgenic mice. | Buard, J., et al. 2000. Genomics. 65: 95-103. PMID: 10783256
- Strand-separating conformational polymorphism analysis: efficacy of detection of point mutations in the human ornithine delta-aminotransferase gene. | Michaud, J., et al. 1992. Genomics. 13: 389-94. PMID: 1612597
- Ornithine aminotransferase deficiency: diagnostic difficulties in neonatal presentation. | Cleary, MA., et al. 2005. J Inherit Metab Dis. 28: 673-9. PMID: 16151897
- Dietary compliance in ornithine aminotransferase deficiency. | Santos, L., et al. 2006. J Inherit Metab Dis. 29: 240. PMID: 16601905
- Cellular response to 5-fluorouracil (5-FU) in 5-FU-resistant colon cancer cell lines during treatment and recovery. | De Angelis, PM., et al. 2006. Mol Cancer. 5: 20. PMID: 16709241
- Ornithine deficiency in the arginase double knockout mouse. | Deignan, JL., et al. 2006. Mol Genet Metab. 89: 87-96. PMID: 16753325
- Molecular pathology of gyrate atrophy of the choroid and retina due to ornithine aminotransferase deficiency. | Ramesh, V., et al. 1991. Mol Biol Med. 8: 81-93. PMID: 1682785
- Human ornithine aminotransferase complexed with L-canaline and gabaculine: structural basis for substrate recognition. | Shah, SA., et al. 1997. Structure. 5: 1067-75. PMID: 9309222
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
hepatic OAT Antibody (D-10) | sc-376050 | 200 µg/ml | $316.00 | |||
hepatic OAT Antibody (D-10): m-IgG Fc BP-HRP Bundle | sc-529811 | 200 µg Ab; 10 µg BP | $354.00 | |||
hepatic OAT Antibody (D-10): m-IgGκ BP-HRP Bundle | sc-522832 | 200 µg Ab, 40 µg BP | $354.00 | |||
hepatic OAT Antibody (D-10): m-IgG1 BP-HRP Bundle | sc-543701 | 200 µg Ab; 20 µg BP | $354.00 | |||
hepatic OAT Antibody (D-10) AC | sc-376050 AC | 500 µg/ml, 25% agarose | $416.00 | |||
hepatic OAT Antibody (D-10) HRP | sc-376050 HRP | 200 µg/ml | $316.00 | |||
hepatic OAT Antibody (D-10) FITC | sc-376050 FITC | 200 µg/ml | $330.00 | |||
hepatic OAT Antibody (D-10) PE | sc-376050 PE | 200 µg/ml | $343.00 | |||
hepatic OAT Antibody (D-10) Alexa Fluor® 488 | sc-376050 AF488 | 200 µg/ml | $357.00 | |||
hepatic OAT Antibody (D-10) Alexa Fluor® 546 | sc-376050 AF546 | 200 µg/ml | $357.00 | |||
hepatic OAT Antibody (D-10) Alexa Fluor® 594 | sc-376050 AF594 | 200 µg/ml | $357.00 | |||
hepatic OAT Antibody (D-10) Alexa Fluor® 647 | sc-376050 AF647 | 200 µg/ml | $357.00 | |||
hepatic OAT Antibody (D-10) Alexa Fluor® 680 | sc-376050 AF680 | 200 µg/ml | $357.00 | |||
hepatic OAT Antibody (D-10) Alexa Fluor® 790 | sc-376050 AF790 | 200 µg/ml | $357.00 |