OAT Antibody (A-12): sc-374243

OAT Antibody (A-12) is a mouse monoclonal IgG2a kappa light chain antibody that detects ornithine aminotransferase (OAT) in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). OAT antibody (A-12) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Ornithine aminotransferase (OAT) is a crucial enzyme composed of 439 amino acids, encoded by the human gene OAT, and belongs to the class III pyridoxal-phosphate-dependent aminotransferase family. OAT is primarily located in the mitochondrial matrix, where OAT functions as a homotetramer, catalyzing the conversion of ornithine to other metabolites, which is vital for the urea cycle and amino acid metabolism. Proper functioning of OAT is essential, as deficiencies can lead to ornithinemia, a condition associated with gyrate atrophy of the choroid and retina, characterized by progressive vision loss and retinal degeneration. The hepatic form of OAT is generated by the cleavage of a 25 amino acid transit peptide from the N-terminus of its precursor, while the renal form arises from the cleavage of a 35 amino acid transit peptide, highlighting the importance of post-translational modifications in determining OAT′s functional roles in different tissues.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

OAT Antibody (A-12) References:

1. Somatic versus germline mutation processes at minisatellite CEB1 (D2S90) in humans and transgenic mice.  |  Buard, J., et al. 2000. Genomics. 65: 95-103. PMID: 10783256
2. Strand-separating conformational polymorphism analysis: efficacy of detection of point mutations in the human ornithine delta-aminotransferase gene.  |  Michaud, J., et al. 1992. Genomics. 13: 389-94. PMID: 1612597
3. Ornithine aminotransferase deficiency: diagnostic difficulties in neonatal presentation.  |  Cleary, MA., et al. 2005. J Inherit Metab Dis. 28: 673-9. PMID: 16151897
4. Dietary compliance in ornithine aminotransferase deficiency.  |  Santos, L., et al. 2006. J Inherit Metab Dis. 29: 240. PMID: 16601905
5. Cellular response to 5-fluorouracil (5-FU) in 5-FU-resistant colon cancer cell lines during treatment and recovery.  |  De Angelis, PM., et al. 2006. Mol Cancer. 5: 20. PMID: 16709241
6. Ornithine deficiency in the arginase double knockout mouse.  |  Deignan, JL., et al. 2006. Mol Genet Metab. 89: 87-96. PMID: 16753325
7. Molecular pathology of gyrate atrophy of the choroid and retina due to ornithine aminotransferase deficiency.  |  Ramesh, V., et al. 1991. Mol Biol Med. 8: 81-93. PMID: 1682785
8. Human ornithine aminotransferase complexed with L-canaline and gabaculine: structural basis for substrate recognition.  |  Shah, SA., et al. 1997. Structure. 5: 1067-75. PMID: 9309222

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-OAT Antibody (A-12) has 5 citations in a variety of scientific publications.

Primary antibodies like Anti-OAT Antibody (A-12) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

OAT Antibody (A-12) Specifications

Alternate Product Name: OAT

Product Clone Name: A-12

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 96-230

Epitope Target: OAT

Epitope Species: human

Product Reactivity/Detection: hepatic and renal forms of ornithine aminotransferase

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 5 citations in various scientific publications

Available Conjugates/Forms for OAT Antibody (A-12): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin