Hemoglobin γ Antibody (51-7): sc-21756

Hemoglobin γ Antibody (51-7) is a mouse monoclonal IgG1 kappa light chain antibody raised against human Hemoglobin gamma, also known as Hemoglobin γ, HBG1, or HBG2. Hemoglobin γ monoclonal antibody (51-7) specifically detects Hemoglobin γ of human origin using techniques such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and flow cytometry (FCM). Hemoglobin gamma is a globin protein subunit that forms part of fetal hemoglobin (Hb F), which consists of two alpha (α) chains and two gamma (γ) chains, and is the predominant form of hemoglobin during fetal development. Located in the cytoplasm of red blood cells, Hemoglobin γ plays a crucial role in oxygen transport from the mother to the fetus due to its higher affinity for oxygen compared to adult hemoglobin. The cellular localization in the cytoplasm allows for efficient oxygen binding and release within erythrocytes, facilitating adequate oxygen supply to rapidly developing fetal tissues. After birth, Hemoglobin γ expression decreases as beta (β) chains gradually replace gamma chains to form adult hemoglobin (Hb A). Studying Hemoglobin γ is essential for understanding fetal development and investigating disorders such as hereditary persistence of fetal hemoglobin and hemoglobinopathies. Hemoglobin γ (51-7) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates, offering versatility for a wide range of experimental applications.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Hemoglobin γ Antibody (51-7) References:

1. N-terminal contributions of the gamma-subunit of fetal hemoglobin to its tetramer strength: remote effects at subunit contacts.  |  Yagami, T., et al. 2002. Protein Sci. 11: 27-35. PMID: 11742119
2. Assembly of human hemoglobin (Hb) beta- and gamma-globin chains expressed in a cell-free system with alpha-globin chains to form Hb A and Hb F.  |  Adachi, K., et al. 2002. J Biol Chem. 277: 13415-20. PMID: 11827978
3. Analysis of hemoglobin gamma chain in patients with Cooley's anemia in Taiwan.  |  Lin, LI., et al. 1992. J Formos Med Assoc. 91: 121-5. PMID: 1285319
4. Linkage of interactions in sickle hemoglobin fiber assembly: inhibitory effect emanating from mutations in the AB region of the alpha-chain is annulled by a mutation at its EF corner.  |  Sudha, R., et al. 2004. J Biol Chem. 279: 20018-27. PMID: 14982923
5. Transfer of human alpha- to beta-hemoglobin via its chaperone protein: evidence for a new state.  |  Baudin-Creuza, V., et al. 2004. J Biol Chem. 279: 36530-3. PMID: 15220346
6. Molecular mechanism of AHSP-mediated stabilization of alpha-hemoglobin.  |  Feng, L., et al. 2004. Cell. 119: 629-40. PMID: 15550245
7. Quantitative analysis of cellular fetal hemoglobin gamma chain messenger RNA (HbF-gamma mRNA) in maternal peripheral blood.  |  Okuda, T., et al. 2004. Prenat Diagn. 24: 881-6. PMID: 15565590
8. Induction of tolerogenic vs immunogenic dendritic cells (DCs) in the presence of GM-CSF is regulated by the strength of signaling from monophosphoryl lipid A (MPLA) in association with glutathione and fetal hemoglobin gamma-chain.  |  Khatri, I., et al. 2009. Immunol Lett. 124: 44-9. PMID: 19379773
9. Molecular basis of length polymorphism in the human zeta-globin gene complex.  |  Goodbourn, SE., et al. 1983. Proc Natl Acad Sci U S A. 80: 5022-6. PMID: 6308667
10. Homology and concerted evolution at the alpha 1 and alpha 2 loci of human alpha-globin.  |  Liebhaber, SA., et al. 1981. Nature. 290: 26-9. PMID: 7010180
11. German population data on the loci low-density-lipoprotein receptor, glycophorin A, hemoglobin gamma G, D7S8 and group-specific component.  |  Huckenbeck, W., et al. 1995. Anthropol Anz. 53: 193-8. PMID: 7486878
12. The multiple functions of hemoglobin.  |  Giardina, B., et al. 1995. Crit Rev Biochem Mol Biol. 30: 165-96. PMID: 7555018

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Hemoglobin γ Antibody (51-7) has 60 citations in a variety of scientific publications.

Primary antibodies like Anti-Hemoglobin γ Antibody (51-7) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Hemoglobin γ Antibody (51-7) Specifications

Alternate Product Name: Hemoglobin gamma (HBG1 / HBG2)

Product Clone Name: 51-7

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids:

Epitope Target: Hemoglobin γ

Epitope Species: human

Product Reactivity/Detection: Hemoglobin γ

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), FCM

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 60 citations in various scientific publications

Available Conjugates/Forms for Hemoglobin γ Antibody (51-7): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred Hemoglobin β antibody is Hemoglobin β Antibody (37-8): sc-21757.