Hemoglobin β/γ/δ/ε Antibody (A-8): sc-390668

Hemoglobin β/γ/δ/ε Antibody (A-8) is a mouse monoclonal IgG1 kappa light chain antibody that specifically targets amino acids 13-46 of human Hemoglobin β, γ, δ, and ε chains, as well as their mouse and rat homologs, including Beta-s, Hbb-b2, and Hbb-y. These globin subunits, also known by their gene symbols HBB (beta), HBG1/HBG2 (gamma), HBD (delta), and HBE1 (epsilon), are essential components of hemoglobin, the oxygen-carrying protein in red blood cells. Each hemoglobin molecule is a tetramer composed of two alpha (α) chains and two non-alpha chains (β, γ, δ, or ε), and the specific combination of these chains varies during different developmental stages, influencing oxygen affinity. The combination of these chains determines the type of hemoglobin present, such as HbA (α2β2), HbF (α2γ2), HbA2 (α2δ2), and embryonic hemoglobins like Hb Gower-1 (ζ2ε2). Hemoglobin β/γ/δ/ε (A-8) antibody recognizes multiple hemoglobin subunits, making anti-Hemoglobin β/γ/δ/ε antibody (A-8) valuable for studying hemoglobin synthesis, function, and related disorders such as anemia and hemoglobinopathies. Hemoglobin β/γ/δ/ε (A-8) monoclonal antibody is validated for use in western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA), providing versatility for various research applications. By targeting conserved regions across different hemoglobin subunits, Hemoglobin β/γ/δ/ε (A-8) antibody aids in the investigation of oxygen transport mechanisms and the regulation of hemoglobin expression during development and disease.

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Hemoglobin β/γ/δ/ε Antibody (A-8) References:

1. Assembly of human hemoglobin (Hb) beta- and gamma-globin chains expressed in a cell-free system with alpha-globin chains to form Hb A and Hb F.  |  Adachi, K., et al. 2002. J Biol Chem. 277: 13415-20. PMID: 11827978
2. [Hemoglobin, from microorganisms to man: a single structural motif, multiple functions].  |  Wajcman, H. and Kiger, L. 2002. C R Biol. 325: 1159-74. PMID: 12520866
3. Clinical manifestations of inherited abnormal hemoglobins. I. The interaction of hemoglobin-S with hemoglobin-D.  |  STURGEON, P., et al. 1955. Blood. 10: 389-404. PMID: 14363320
4. Linkage of interactions in sickle hemoglobin fiber assembly: inhibitory effect emanating from mutations in the AB region of the alpha-chain is annulled by a mutation at its EF corner.  |  Sudha, R., et al. 2004. J Biol Chem. 279: 20018-27. PMID: 14982923
5. Transfer of human alpha- to beta-hemoglobin via its chaperone protein: evidence for a new state.  |  Baudin-Creuza, V., et al. 2004. J Biol Chem. 279: 36530-3. PMID: 15220346
6. Molecular mechanism of AHSP-mediated stabilization of alpha-hemoglobin.  |  Feng, L., et al. 2004. Cell. 119: 629-40. PMID: 15550245
7. [Role of alpha-hemoglobin molecular chaperone in the hemoglobin formation and clinical expression of some hemoglobinopathies].  |  Vasseur, C. and Baudin-Creuza, V. 2015. Transfus Clin Biol. 22: 49-57. PMID: 25724329
8. Affinity and Effect of Anticancer Drugs on the Redox Reactivity of Hemoglobin.  |  Puscas, C., et al. 2019. Chem Res Toxicol. 32: 1402-1411. PMID: 31268688
9. Developmental genetics of the human haemoglobins.  |  Wood, WG. and Weatherall, DJ. 1983. Biochem J. 215: 1-10. PMID: 6194789
10. Molecular basis of length polymorphism in the human zeta-globin gene complex.  |  Goodbourn, SE., et al. 1983. Proc Natl Acad Sci U S A. 80: 5022-6. PMID: 6308667
11. Homology and concerted evolution at the alpha 1 and alpha 2 loci of human alpha-globin.  |  Liebhaber, SA., et al. 1981. Nature. 290: 26-9. PMID: 7010180
12. The multiple functions of hemoglobin.  |  Giardina, B., et al. 1995. Crit Rev Biochem Mol Biol. 30: 165-96. PMID: 7555018

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Hemoglobin β/γ/δ/ε Antibody (A-8) has 6 citations in a variety of scientific publications.

Primary antibodies like Anti-Hemoglobin β/γ/δ/ε Antibody (A-8) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Hemoglobin β/γ/δ/ε Antibody (A-8) Specifications

Alternate Product Name: Hemoglobin

Product Clone Name: A-8

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 13-46

Epitope Target: Hemoglobin β, Hemoglobin γ, Hemoglobin δ and Hemoglobin ε of human origin, Beta-s, Hbb-b2, and Hbb-y of mouse origin, and the corresponding rat homologs

Epitope Species: human

Product Reactivity/Detection: Hemoglobin β, Hemoglobin γ, Hemoglobin δ and Hemoglobin ε of human origin, β-s, Hbb-b2, and Hbb-y of mouse origin, and the corresponding rat homologs

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 6 citations in various scientific publications

Available Conjugates/Forms for Hemoglobin β/γ/δ/ε Antibody (A-8): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin

The preferred Hemoglobin β antibody is Hemoglobin β Antibody (37-8): sc-21757.