Hemoglobin beta / gamma / delta / epsilon Antibody (A-8) detects Hemoglobin β, γ, δ and ε of human origin, β-s, Hbb-b2, and Hbb-y of mouse origin, and the corresponding rat homologs by WB, IP, IF, IHC(P) and ELISA

Hemoglobin β/γ/δ/ε Antibody (A-8): sc-390668

Hemoglobin β/γ/δ/ε Antibody (A-8) is rated 4.5 out of 5 by 4.
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Datasheets

    • Anti-Hemoglobin β/γ/δ/ε Antibody (A-8) is a mouse monoclonal IgG1 (kappa light chain) Hemoglobin β/γ/δ/ε antibody provided at 200 µg/ml
    • specific for an epitope mapping between amino acids 13-46 of Hemoglobin β, Hemoglobin γ, Hemoglobin δ and Hemoglobin ε of human origin, Beta-s, Hbb-b2, and Hbb-y of mouse origin, and the corresponding rat homologs of human origin
    • Hemoglobin beta / gamma / delta / epsilon Antibody (A-8) is recommended for detection of Hemoglobin β, Hemoglobin γ, Hemoglobin δ and Hemoglobin ε of human origin, β-s, Hbb-b2, and Hbb-y of mouse origin, and the corresponding rat homologs by WB, IP, IF, IHC(P) and ELISA
    • Anti-Hemoglobin beta / gamma / delta / epsilon Antibody (A-8) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • blocking peptide, sc-390668 P
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Hemoglobin β/γ/δ/ε (A-8): sc-390668.

What application is the blocking peptide sc-390668 P appropriate for?

Asked by: DefinitelyNotMatt
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-24
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Rated 3 out of 5 by from Not working for flow cytometry assay I recently bought two antibodies, this one and the other one against hemoglobin beta. I tried two different types of fixation: formaldehyde and glutaraldehyde. Both antibodies were not working with glutaraldehyde fixation. However, with formaldehyde fixation, the anti-hemoglobin beta worked nicely, not this antibody. You can see a shift of fluorescence intensity upon adding this antibody but it was just non-specific signal. Any thoughts or suggestion to get it work?
Date published: 2020-04-15
Rated 5 out of 5 by from Clear staining! Bought it a year ago and I am very pleased. Been using it at concentration 1:1000 to detect hemoglobin upregulation during erythroid differentiation in WB. Highly recommend it!
Date published: 2019-05-16
Rated 5 out of 5 by from excellent antibody I used this antibody for the pig tissue IF staining. I got perfect images!
Date published: 2017-11-04
Rated 5 out of 5 by from Produced positive Western blot data of Hemoglobin Produced positive Western blot data of Hemoglobin expression in mouse heart tissue extract. -SCBT QC
Date published: 2014-07-11
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