Hemoglobin α Antibody (D-4): sc-514378

Hemoglobin α Antibody (D-4) is a mouse monoclonal IgG1 kappa light chain antibody that detects the Hemoglobin alpha protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-Hemoglobin α antibody (D-4) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Hemoglobin, a crucial protein in the body, is responsible for transporting oxygen from the lungs to tissues and facilitating the return of carbon dioxide from tissues back to the lungs. Hemoglobin′s structure consists of two alpha and two beta chains that form a tetramer, with each chain binding to a heme group that contains iron. This arrangement allows hemoglobin to undergo conformational changes that significantly affect oxygen-binding affinity. The α1-β2 cleavage plane plays a critical role in this process, as the interaction between the chains can either enhance or reduce oxygen binding, depending on whether the structure is in a relaxed or tense state. The majority of circulating hemoglobin is Hb A, which is composed of two alpha and two beta chains, while smaller amounts consist of Hb A-2 and fetal hemoglobin, highlighting Hemoglobin alpha′s importance in overall oxygen transport and delivery in the human body.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Hemoglobin α Antibody (D-4) References:

1. Assembly of human hemoglobin (Hb) beta- and gamma-globin chains expressed in a cell-free system with alpha-globin chains to form Hb A and Hb F.  |  Adachi, K., et al. 2002. J Biol Chem. 277: 13415-20. PMID: 11827978
2. Linkage of interactions in sickle hemoglobin fiber assembly: inhibitory effect emanating from mutations in the AB region of the alpha-chain is annulled by a mutation at its EF corner.  |  Sudha, R., et al. 2004. J Biol Chem. 279: 20018-27. PMID: 14982923
3. Transfer of human alpha- to beta-hemoglobin via its chaperone protein: evidence for a new state.  |  Baudin-Creuza, V., et al. 2004. J Biol Chem. 279: 36530-3. PMID: 15220346
4. Molecular mechanism of AHSP-mediated stabilization of alpha-hemoglobin.  |  Feng, L., et al. 2004. Cell. 119: 629-40. PMID: 15550245
5. Hemoglobin α in the blood vessel wall.  |  Butcher, JT., et al. 2014. Free Radic Biol Med. 73: 136-42. PMID: 24832680
6. Role of hemoglobin alpha and hemoglobin beta in non-small-cell lung cancer based on bioinformatics analysis.  |  Kang, N., et al. 2022. Mol Carcinog. 61: 587-602. PMID: 35394695
7. Hemoglobin Alpha Chain Variant Zara Associated With Familial Asymptomatic Hypoxemia.  |  Pasquarella, A., et al. 2022. J Hematol. 11: 190-195. PMID: 36406831
8. The C-terminal 32-mer fragment of hemoglobin alpha is an amyloidogenic peptide with antimicrobial properties.  |  Olari, LR., et al. 2023. Cell Mol Life Sci. 80: 151. PMID: 37198527
9. Hemoglobin alpha is a redox-sensitive mitochondrial-related protein in T-lymphocytes.  |  Reed, EC., et al. 2024. Free Radic Biol Med. 227: 1-11. PMID: 39586383
10. Molecular basis of length polymorphism in the human zeta-globin gene complex.  |  Goodbourn, SE., et al. 1983. Proc Natl Acad Sci U S A. 80: 5022-6. PMID: 6308667
11. Homology and concerted evolution at the alpha 1 and alpha 2 loci of human alpha-globin.  |  Liebhaber, SA., et al. 1981. Nature. 290: 26-9. PMID: 7010180
12. The multiple functions of hemoglobin.  |  Giardina, B., et al. 1995. Crit Rev Biochem Mol Biol. 30: 165-96. PMID: 7555018

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Hemoglobin α Antibody (D-4) has 15 citations in a variety of scientific publications.

Primary antibodies like Anti-Hemoglobin α Antibody (D-4) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Hemoglobin α Antibody (D-4) Specifications

Alternate Product Name: Hemoglobin alpha (HBA)

Product Clone Name: D-4

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 62-142

Epitope Target: Hemoglobin α

Epitope Species: human

Product Reactivity/Detection: Hemoglobin α

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 15 citations in various scientific publications

Available Conjugates/Forms for Hemoglobin α Antibody (D-4): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin