Heme Oxygenase 1 Antibody (F-4) recommended for detection of Heme Oxygenase 1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

Heme Oxygenase 1 Antibody (F-4): sc-390991

Heme Oxygenase 1 Antibody (F-4) is rated 3.8 out of 5 by 14.
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Datasheets

    • Heme Oxygenase 1 Antibody (F-4) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • specific for an epitope mapping between amino acids 245-283 near the C-terminus of Heme Oxygenase 1 of human origin
    • Anti-Heme Oxygenase 1 Antibody (F-4) is recommended for detection of Heme Oxygenase 1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including and canine and bovine
    • Anti-Heme Oxygenase 1 Antibody (F-4) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • blocking peptide, sc-390991 P
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Heme Oxygenase 1 (F-4): sc-390991.

I would like to do some IHC on my mouse samples. Do you have any suggestions on how this can be successful using a mouse monoclonal primary?

Asked by: Cweed
We recommend using a a directly conjugated primary antibodies. Please contact our special orders department for pricing and availability of sc-390991 conjugates.
Answered by: Technical Service 11
Date published: 2017-01-12

I have some zebrafish samples that I want to test, will this antibody cross-react?

Asked by: Cweed
Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity.
Answered by: Technical Service 11
Date published: 2017-01-12
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Rated 4 out of 5 by from Good staining 40 µgs whole cell lysate RAW 264.7 Polyacrilamide gel 12% Ab dilution 1:500 Secondary Ab: goat anti mouse-HRP BioRad 1:5000 Recognizes non specific bands at 75 and 22 kDa Gives some background
Date published: 2019-04-04
Rated 5 out of 5 by from Good antibody for WB and IF The antibody works pretty well for Western blotting, showing a clear band with minimal background. It also works well for immunofluorescence staining.
Date published: 2019-03-14
Rated 3 out of 5 by from OK antibody Worked ok. However, not as great as other SC primaries that I have used.
Date published: 2018-03-23
Rated 2 out of 5 by from 条带不清晰,基本上没曝出来 稀释比例调到1比100,也没有曝出来条带,抗体效价有点低。。。。。。。。。。。。。。。。。。。。。。。。。。。。。。。。。。。。。。。。。。。。。。。。。。。。。。。。。。。。。。。。。。。。。。。。。。。。。。。。。。。。。。。。。。。。。。。。。。。。。。。。。。。。。。。。。。。。
Date published: 2017-12-26
Rated 4 out of 5 by from No band detected in U87 cell lysates (WB) No visible band for western blot with 25 ug U87 cell lysates at 1:1000 primary antibody dilution (fluorescence detection).
Date published: 2017-09-11
Rated 2 out of 5 by from Not so great Unlike sc-136960 (which is an excellent antibody), this antibody does not seem to recognizes a band of predicted molecular weight on the endogeneous level in our hands (1:500 dilution,t30 ug HeLa total extract).
Date published: 2017-08-24
Rated 4 out of 5 by from Good antibody The antibody works well for western blot 1:100 with rat lungs tissue.
Date published: 2017-08-08
Rated 3 out of 5 by from There are two main bands ,I don`t know which one 1:500 diluted for wb of rat protein ,the band is clear,but there are two main bands ,one is above 30KD ,the other is below 30KD ,both of them are clear,I don`t which one is the right one!
Date published: 2017-07-29
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