HEB Antibody (D-3): sc-28364

HEB Antibody (D-3) is a mouse monoclonal IgG1 kappa light chain antibody that detects HEB protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). anti-HEB antibody (D-3) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. HEB protein, a member of the helix-loop-helix (HLH) family, plays a crucial role in regulating myogenic differentiation, which is essential for muscle development and regeneration. Specifically, HEB interacts with other basic HLH proteins, such as Myo D, to form heterodimers that bind to E box elements in regulatory regions of muscle-specific genes. This interaction is vital for transcriptional activation of genes involved in muscle cell lineage commitment and differentiation. HEB′s ability to form both homo- and hetero-oligomers allows for precise regulation of gene expression, ensuring muscle cells develop correctly in response to various signaling pathways. Using HEB (D-3) antibody in your research can provide insights into molecular mechanisms governing muscle development and intricate network of protein interactions that facilitate this process.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

HEB Antibody (D-3) References:

1. HEB, a helix-loop-helix protein related to E2A and ITF2 that can modulate the DNA-binding ability of myogenic regulatory factors.  |  Hu, JS., et al. 1992. Mol Cell Biol. 12: 1031-42. PMID: 1312219
2. Molecular characterization of helix-loop-helix peptides.  |  Anthony-Cahill, SJ., et al. 1992. Science. 255: 979-83. PMID: 1312255
3. HEB in the spotlight: Transcriptional regulation of T-cell specification, commitment, and developmental plasticity.  |  Braunstein, M. and Anderson, MK. 2012. Clin Dev Immunol. 2012: 678705. PMID: 22577461
4. Herculin, a fourth member of the MyoD family of myogenic regulatory genes.  |  Miner, JH. and Wold, B. 1990. Proc Natl Acad Sci U S A. 87: 1089-93. PMID: 2300571
5. Interactions between heterologous helix-loop-helix proteins generate complexes that bind specifically to a common DNA sequence.  |  Murre, C., et al. 1989. Cell. 58: 537-44. PMID: 2503252
6. Myogenin, a factor regulating myogenesis, has a domain homologous to MyoD.  |  Wright, WE., et al. 1989. Cell. 56: 607-17. PMID: 2537150
7. Identification of MRF4: a new member of the muscle regulatory factor gene family.  |  Rhodes, SJ. and Konieczny, SF. 1989. Genes Dev. 3: 2050-61. PMID: 2560751
8. A novel human muscle factor related to but distinct from MyoD1 induces myogenic conversion in 10T1/2 fibroblasts.  |  Braun, T., et al. 1989. EMBO J. 8: 701-9. PMID: 2721498
9. HEB is required for the specification of fetal IL-17-producing γδ T cells.  |  In, TSH., et al. 2017. Nat Commun. 8: 2004. PMID: 29222418
10. TCF-1 and HEB cooperate to establish the epigenetic and transcription profiles of CD4+CD8+ thymocytes.  |  Emmanuel, AO., et al. 2018. Nat Immunol. 19: 1366-1378. PMID: 30420627
11. Monoallelic Heb/Tcf12 Deletion Reduces the Requirement for NOTCH1 Hyperactivation in T-Cell Acute Lymphoblastic Leukemia.  |  Veiga, DFT., et al. 2022. Front Immunol. 13: 867443. PMID: 35401501
12. Cell-specific expression of helix-loop-helix transcription factors encoded by the E2A gene.  |  Aronheim, A., et al. 1993. Nucleic Acids Res. 21: 1601-6. PMID: 8479911

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-HEB Antibody (D-3) has 8 citations in a variety of scientific publications.

Primary antibodies like Anti-HEB Antibody (D-3) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

HEB Antibody (D-3) Specifications

Alternate Product Name: HEB

Product Clone Name: D-3

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 31-160

Epitope Target: HEB

Epitope Species: human

Product Reactivity/Detection: HEB

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 8 citations in various scientific publications

Available Conjugates/Forms for HEB Antibody (D-3): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin, X: TransCruz Reagent