HEB Antibody (C-7): sc-365572
- HEB Antibody (C-7) is a mouse monoclonal IgG1 κ provided at 200 µg/ml
- specific for an epitope mapping between amino acids 652-683 within the C-terminus of HEB of human origin
- recommended for detection of HEB of mouse, rat and human origin by WB, IP, IF and ELISA; also reactive with additional species, including and equine and bovine
- TransCruz reagent for ChIP application (sc-365572 X, 200 µg/0.1 ml)
- m-IgG Fc BP-HRP is the preferred secondary detection reagent for HEB Antibody (C-7) for WB applications. This reagent is now offered in a bundle with HEB Antibody (C-7) (see ordering information below).
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HEB Antibody (C-7) is a mouse monoclonal IgG1 antibody that detects HEB in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). HEB, a member of the basic helix-loop-helix (bHLH) protein family, plays a crucial role in the regulation of gene expression during muscle cell differentiation. HEB (C-7) antibody targets proteins primarily located in the nucleus, where HEB functions as a transcription factor that forms heterodimers with other bHLH proteins, such as Myo D, to bind to E box elements in the promoter regions of muscle-specific genes. This interaction is vital for the activation of genes necessary for muscle development and differentiation, highlighting HEB′s importance in myogenesis and muscle regeneration. The ability of HEB to form both homo- and hetero-oligomers allows for a fine-tuned regulation of gene expression, ensuring that muscle cells develop correctly and efficiently. Anti-HEB antibody (C-7) serves as an essential tool for researchers studying muscle biology and the molecular mechanisms underlying muscle differentiation and development.
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HEB Antibody (C-7) References:
- Functions of E2A-HEB heterodimers in T-cell development revealed by a dominant negative mutation of HEB. | Barndt, RJ., et al. 2000. Mol Cell Biol. 20: 6677-85. PMID: 10958665
- Regulation of pT alpha gene expression by a dosage of E2A, HEB, and SCL. | Tremblay, M., et al. 2003. J Biol Chem. 278: 12680-7. PMID: 12566462
- Structure of the AML1-ETO eTAFH domain-HEB peptide complex and its contribution to AML1-ETO activity. | Park, S., et al. 2009. Blood. 113: 3558-67. PMID: 19204326
- HEB-deficient T-cell precursors lose T-cell potential and adopt an alternative pathway of differentiation. | Braunstein, M. and Anderson, MK. 2011. Mol Cell Biol. 31: 971-82. PMID: 21189289
- HEB and E2A function as SMAD/FOXH1 cofactors. | Yoon, SJ., et al. 2011. Genes Dev. 25: 1654-61. PMID: 21828274
- HEB associates with PRC2 and SMAD2/3 to regulate developmental fates. | Yoon, SJ., et al. 2015. Nat Commun. 6: 6546. PMID: 25775035
- HEB silencing induces anti-proliferative effects on U87MG cells cultured as neurospheres and monolayers. | Godoy, PR., et al. 2016. Mol Med Rep. 14: 5253-5260. PMID: 27779678
- Combinatorial transcriptional regulation of HEB/ZEB1/ASCL1 and MYBL2 on Ras/ErbB signaling. | Zhong, F., et al. 2022. Biochem Biophys Res Commun. 622: 170-176. PMID: 35932528
- Stabilization of β-Catenin Directs HEB to Limit Thymic Selection. | Tousinas, G., et al. 2024. J Immunol. 213: 641-650. PMID: 38958395
- Functional replacement of the mouse E2A gene with a human HEB cDNA. | Zhuang, Y., et al. 1998. Mol Cell Biol. 18: 3340-9. PMID: 9584174
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
HEB Antibody (C-7) | sc-365572 | 200 µg/ml | $322.00 | |||
HEB Antibody (C-7): m-IgG Fc BP-HRP Bundle | sc-540349 | 200 µg Ab; 10 µg BP | $361.00 | |||
HEB (C-7) Neutralizing Peptide | sc-365572 P | 100 µg/0.5 ml | $69.00 | |||
HEB Antibody (C-7) X | sc-365572 X | 200 µg/0.1 ml | $322.00 |