HEB Antibody (A-6): sc-365980

HEB Antibody (A-6) is a mouse monoclonal IgG3 antibody that detects HEB in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). HEB, a member of the basic helix-loop-helix (bHLH) protein family, plays a crucial role in the regulation of gene expression during muscle cell differentiation. HEB (A-6) antibody targets proteins primarily located in the nucleus, where HEB forms heterodimers with other bHLH proteins, such as Myo D, to bind to E box elements in the promoter regions of muscle-specific genes. This interaction is vital for the activation of genes necessary for muscle development, highlighting HEB′s importance in myogenesis and muscle cell lineage specification. The ability of HEB to form both homo- and hetero-oligomers allows for precise regulation of gene expression, ensuring that muscle cells differentiate correctly in response to developmental cues. Anti-HEB antibody (A-6) serves as an essential tool for researchers studying muscle biology and the molecular mechanisms underlying muscle differentiation and development.

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HEB Antibody (A-6) References:

1. SCL and LMO1 alter thymocyte differentiation: inhibition of E2A-HEB function and pre-T alpha chain expression.  |  Herblot, S., et al. 2000. Nat Immunol. 1: 138-44. PMID: 11248806
2. E2A and HEB activate the pre-TCR alpha promoter during immature T cell development.  |  Takeuchi, A., et al. 2001. J Immunol. 167: 2157-63. PMID: 11490000
3. HEB, a helix-loop-helix protein related to E2A and ITF2 that can modulate the DNA-binding ability of myogenic regulatory factors.  |  Hu, JS., et al. 1992. Mol Cell Biol. 12: 1031-42. PMID: 1312219
4. TAL1/SCL induces leukemia by inhibiting the transcriptional activity of E47/HEB.  |  O'Neil, J., et al. 2004. Cancer Cell. 5: 587-96. PMID: 15193261
5. Acquisition of a functional T cell receptor during T lymphocyte development is enforced by HEB and E2A transcription factors.  |  Jones, ME. and Zhuang, Y. 2007. Immunity. 27: 860-70. PMID: 18093538
6. CBFA2T3-ZNF652 corepressor complex regulates transcription of the E-box gene HEB.  |  Kumar, R., et al. 2008. J Biol Chem. 283: 19026-38. PMID: 18456661
7. AML1/ETO oncoprotein is directed to AML1 binding regions and co-localizes with AML1 and HEB on its targets.  |  Gardini, A., et al. 2008. PLoS Genet. 4: e1000275. PMID: 19043539
8. An essential role for the transcription factor HEB in thymocyte survival, Tcra rearrangement and the development of natural killer T cells.  |  D'Cruz, LM., et al. 2010. Nat Immunol. 11: 240-9. PMID: 20154672
9. The transcription factors E2A and HEB act in concert to induce the expression of FOXO1 in the common lymphoid progenitor.  |  Welinder, E., et al. 2011. Proc Natl Acad Sci U S A. 108: 17402-7. PMID: 21972416
10. A heterodimer of HEB and an E12-related protein interacts with the CD4 enhancer and regulates its activity in T-cell lines.  |  Sawada, S. and Littman, DR. 1993. Mol Cell Biol. 13: 5620-8. PMID: 8355705

The preferred HEB antibody is HEB Antibody (D-3): sc-28364.