Histone Deacetylase 8 (HDAC8) Antibody (E-5): sc-17778

HDAC8 Antibody (E-5) is a mouse monoclonal IgG2b kappa light chain antibody that detects HDAC8 of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry (IHC), and enzyme-linked immunosorbent assay (ELISA). HDAC8 (E-5) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase, phycoerythrin, fluorescein isothiocyanate, and multiple Alexa Fluor® conjugates. HDAC8, a member of the histone deacetylase family, is primarily located in the nucleus and plays a crucial role in the regulation of gene expression through the modification of chromatin structure. By removing acetyl groups from lysine residues on histones, HDAC8 contributes to the condensation of chromatin, leading to transcriptional repression. This process is vital for maintaining cellular homeostasis and regulating developmental processes, as aberrant HDAC activity has been implicated in various cancers and other diseases. HDAC8 interacts with several other proteins, including transcription factors and co-repressors, which further modulates gene regulation. Understanding HDAC8 function and interactions is essential for elucidating contributions to cellular processes and potential as a therapeutic target in cancer treatment.

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Histone Deacetylase 8 (HDAC8) Antibody (E-5) References:

1. Cloning and characterization of a novel human class I histone deacetylase that functions as a transcription repressor.  |  Hu, E., et al. 2000. J Biol Chem. 275: 15254-64. PMID: 10748112
2. Expression of histone deacetylase 8, a class I histone deacetylase, is restricted to cells showing smooth muscle differentiation in normal human tissues.  |  Waltregny, D., et al. 2004. Am J Pathol. 165: 553-64. PMID: 15277229
3. A selective HDAC8 inhibitor potentiates antitumor immunity and efficacy of immune checkpoint blockade in hepatocellular carcinoma.  |  Yang, W., et al. 2021. Sci Transl Med. 13: PMID: 33827976
4. Foot-and-mouth disease virus structural protein VP3 interacts with HDAC8 and promotes its autophagic degradation to facilitate viral replication.  |  Zhang, H., et al. 2023. Autophagy. 19: 2869-2883. PMID: 37408174
5. HDAC3 and HDAC8 PROTAC dual degrader reveals roles of histone acetylation in gene regulation.  |  Xiao, Y., et al. 2023. Cell Chem Biol. 30: 1421-1435.e12. PMID: 37572669
6. Nucleosome structural changes due to acetylation.  |  Bauer, WR., et al. 1994. J Mol Biol. 236: 685-90. PMID: 8114086
7. A positive role for histone acetylation in transcription factor access to nucleosomal DNA.  |  Lee, DY., et al. 1993. Cell. 72: 73-84. PMID: 8422685
8. Transcriptional silencing in yeast is associated with reduced nucleosome acetylation.  |  Braunstein, M., et al. 1993. Genes Dev. 7: 592-604. PMID: 8458576
9. Nucleosomal DNA regulates the core-histone-binding subunit of the human Hat1 acetyltransferase.  |  Verreault, A., et al. 1998. Curr Biol. 8: 96-108. PMID: 9427644
10. Transcriptional activators direct histone acetyltransferase complexes to nucleosomes.  |  Utley, RT., et al. 1998. Nature. 394: 498-502. PMID: 9697775
11. Structural snapshots of human HDAC8 provide insights into the class I histone deacetylases.  |  Somoza, JR., et al. 2004. Structure. 12: 1325-34. PMID: 15242608
12. Histone deacetylase HDAC8 associates with smooth muscle alpha-actin and is essential for smooth muscle cell contractility.  |  Waltregny, D., et al. 2005. FASEB J. 19: 966-8. PMID: 15772115

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Histone Deacetylase 8 (HDAC8) Antibody (E-5) has 16 citations in a variety of scientific publications.

Primary antibodies like Anti-Histone Deacetylase 8 (HDAC8) Antibody (E-5) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Histone Deacetylase 8 (HDAC8) Antibody (E-5) Specifications

Alternate Product Name: HDAC8

Product Clone Name: E-5

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 1-145

Epitope Target: HDAC8

Epitope Species: human

Product Reactivity/Detection: HDAC8

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 16 citations in various scientific publications

Available Conjugates/Forms for Histone Deacetylase 8 (HDAC8) Antibody (E-5): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin