HDAC6 Antibody (D-11) recommended for detection of HDAC6 of human origin by WB, IP, IF, IHC(P) and ELISA

HDAC6 Antibody (D-11): sc-28386

HDAC6 Antibody (D-11) is rated 4.4 out of 5 by 14.
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Datasheets

    • Anti-HDAC6 Antibody (D-11) is a mouse monoclonal IgG2a (kappa light chain) HDAC6 antibody provided at 200 µg/ml
    • raised against amino acids 916-1215 of HDAC6 of human origin
    • HDAC6 Antibody (D-11) is recommended for detection of HDAC6 of human origin by WB, IP, IF, IHC(P) and ELISA
    • Anti-HDAC6 Antibody (D-11) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of HDAC6 (D-11): sc-28386.
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Does this antibody work for mouse tissue (cryosections) IF ?

Asked by: Kate34
Thank you for your question. We do not recommend using this HDAC6 Antibody (D-11): sc-28386 for detection of HDAC6 of mouse origin. It is currently only recommended for detection of HDAC6 of human origin. It is suitable for IF on cryosections for human tissue. Unfortunately, at this time, we do not offer any alternative antibodies against HDAC6 that are recommended for use with mouse samples. Please check back with us in the future as we are constantly adding new antibodies to our product line.
Answered by: Technical Support
Date published: 2018-02-23

What is the total volume of HDAC6 Antibody (D-11): sc-28386 provided?

Asked by: Anna99
Each vial contains 200 µg of mouse monoclonal IgG2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin.
Answered by: Technical Support 4
Date published: 2017-01-09
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Rated 1 out of 5 by from Did not work in WB! I tried this with GBM cell line Whole cell extracts and could not see any bands. I do the same experiment using another brand's antibody and did obtain results.
Date published: 2018-11-22
Rated 4 out of 5 by from Good antibody Worked well with western blotting at 1:500 dilution.
Date published: 2017-09-27
Rated 5 out of 5 by from good quality for WB This antibody works well and generates a clear signal at the predicted position on the western blot (1:500 dilution, 50ug of rat heart extract).
Date published: 2017-09-21
Rated 4 out of 5 by from Antibody was great for WB and IP. Antibody was great for WB and IP in colon cancer, globlastoma and multiple myeloma cells. I recommond this antibody.
Date published: 2017-06-19
Rated 3 out of 5 by from WB signals not so strong I use 1:200 diluted antibody to blot 60 mg total cell lysates from HCT116, K562 and HeLa but the signals were not as strong as shown in the reviews or the datasheets. Could you help me?
Date published: 2017-01-11
Rated 5 out of 5 by from Outstanding Very nice western blot and IHC data;strong signal !!
Date published: 2017-01-06
Rated 5 out of 5 by from good features This product have a good special features for human source cell line (such as Hela, K562, HCT116) by western blot analysis. I sincerely recomand this prouduct.
Date published: 2017-01-04
Rated 5 out of 5 by from WB data looks great Perfect Western signal in a wide variety of samples.
Date published: 2016-12-23
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