Date published: 2025-9-5
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Histone Deacetylase 5 (HDAC5) Antibody (C-11) - Western Blotting - Image 54266
HDAC5 抗体 (C-11): sc-133225. NIH/3T3 (A), KNRK (B), IMR-32 (C) 和 Jurkat (D) 核提取物以及 KNRK (E) 和 COLO 320DM (F) 全细胞裂解液中 HDAC5 表达的 Western 印迹分析.
HDAC5 抗体 (C-11): sc-133225. NIH/3T3 (A), KNRK (B), IMR-32 (C) 和 Jurkat (D) 核提取物以及 KNRK (E) 和 COLO 320DM (F) 全细胞裂解液中 HDAC5 表达的 Western 印迹分析.

Histone Deacetylase 5 (HDAC5) 抗体 (C-11): sc-133225

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寫評論提問

说明书
  • Histone Deacetylase 5 (HDAC5) 抗体 C-11 是小鼠单克隆 IgG2b κ，Histone Deacetylase 5 (HDAC5)抗体, 在35篇文献中引用，规格为200 µg/ml
  • 免疫human物种的HDAC5的氨基酸371-443
  • Histone Deacetylase 5 (HDAC5) 抗体 (C-11) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 HDAC5
  • 抗Histone Deacetylase 5 (HDAC5)抗体（C-11）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Histone Deacetylase 5 (HDAC5) (C-11): sc-133225的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG2b BP-HRP 和 m-IgGκ BP-HRP 是 Histone Deacetylase 5 (HDAC5) 抗体 (C-11) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与Histone Deacetylase 5 (HDAC5) 抗体 (C-11) 组合成套装提供。 (请参阅下面的订购信息)。

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相关产品
描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

额外HDAC抗体，包括HDAC1, HDAC10, HDAC11, HDAC2, HDAC3, HDAC4, HDAC5, HDAC6, HDAC7和HDAC8

HDAC5抗体（C-11）是一种IgG2b κ型小鼠单克隆HDAC5抗体（也称为组蛋白去乙酰化酶5抗体），可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）、免疫组织化学（IHC，P）和酶联免疫吸附测定（ELISA）检测小鼠、大鼠和人类来源的HDAC5蛋白。HDAC5抗体（C-11）既可作为非共轭的抗HDAC5抗体形式提供，也可作为多种共轭形式的抗HDAC5抗体提供，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®共轭物。在完整的细胞中，DNA与组蛋白和其他核蛋白紧密结合形成染色质。人们认为染色质的重塑是转录调控的关键组成部分，而这种重塑的主要来源是核小体组蛋白的乙酰化。组蛋白氨基端尾部结构域的赖氨酸残基乙酰化导致核小体构象发生别构变化，并使DNA更容易接近转录因子。相反，组蛋白的去乙酰化与转录沉默相关。已发现几种哺乳动物蛋白质为核组蛋白乙酰化酶，包括GCN5、PCAF（p300/CBP相关因子）、p300/CBP、HAT1和TFIID亚基TAF II p250。已确定哺乳动物HDAC1（也称为HD1）、HDAC2（也称为RPD3）和HDAC3-6为组蛋白去乙酰化酶。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Histone Deacetylase 5 (HDAC5) 抗体 (C-11) 参考文献:

  1. 三种蛋白定义了一类与酵母 Hda1p 有关的人类组蛋白去乙酰化酶。  |  Grozinger, CM., et al. 1999. Proc Natl Acad Sci U S A. 96: 4868-73. PMID: 10220385
  2. 受miR-148a-3p负调控的HDAC5促进结肠癌细胞迁移  |  OuYang, C., et al. 2022. Cancer Sci. 113: 2560-2574. PMID: 35574707
  3. 抑制 HDAC5 可减轻心室重塑和心功能不全。  |  Zhu, C., et al. 2023. Orphanet J Rare Dis. 18: 266. PMID: 37667300
  4. HDAC5 可增强 IRF3 的激活，并成为包括猴痘病毒和天花病毒在内的正痘病毒的蛋白 C6 的降解靶标。  |  Lu, Y., et al. 2024. Cell Rep. 43: 113788. PMID: 38461415
  5. 乙酰化导致的核小体结构变化  |  Bauer, WR., et al. 1994. J Mol Biol. 236: 685-90. PMID: 8114086
  6. 组蛋白乙酰化在转录因子进入核糖体 DNA 的过程中发挥了积极作用。  |  Lee, DY., et al. 1993. Cell. 72: 73-84. PMID: 8422685
  7. 酵母的转录沉默与核小体乙酰化减少有关。  |  Braunstein, M., et al. 1993. Genes Dev. 7: 592-604. PMID: 8458576
  8. 一种与酵母转录调节因子 Rpd3p 相关的哺乳动物组蛋白去乙酰化酶。  |  Taunton, J., et al. 1996. Science. 272: 408-11. PMID: 8602529
  9. 核糖体 DNA 调节人类 Hat1 乙酰转移酶的核心-活素结合亚基。  |  Verreault, A., et al. 1998. Curr Biol. 8: 96-108. PMID: 9427644
  10. 人类 RPD3 同源物 HDAC3 的特征。  |  Emiliani, S., et al. 1998. Proc Natl Acad Sci U S A. 95: 2795-800. PMID: 9501169
  11. 转录激活剂将组蛋白乙酰转移酶复合物导向核糖体。  |  Utley, RT., et al. 1998. Nature. 394: 498-502. PMID: 9697775
  12. 组蛋白乙酰转移酶和去乙酰化酶在基因调控中的作用。  |  Kuo, MH. and Allis, CD. 1998. Bioessays. 20: 615-26. PMID: 9780836

订购信息

产品名称产品编号规格价格数量收藏夹

Histone Deacetylase 5 (HDAC5) 抗体 (C-11)

sc-133225
200 µg/ml
$316.00

Histone Deacetylase 5 (HDAC5) (C-11): m-IgGκ BP-HRP 套装

sc-521283
200 µg Ab, 40 µg BP
$354.00

Histone Deacetylase 5 (HDAC5) (C-11): m-IgG2b BP-HRP 套装

sc-548882
200 µg Ab; 10 µg BP
$354.00

Histone Deacetylase 5 (HDAC5) 抗体 (C-11) AC

sc-133225 AC
500 µg/ml, 25% agarose
$416.00

Histone Deacetylase 5 (HDAC5) 抗体 (C-11) HRP

sc-133225 HRP
200 µg/ml
$316.00

Histone Deacetylase 5 (HDAC5) 抗体 (C-11) FITC

sc-133225 FITC
200 µg/ml
$330.00

Histone Deacetylase 5 (HDAC5) 抗体 (C-11) PE

sc-133225 PE
200 µg/ml
$343.00

Histone Deacetylase 5 (HDAC5) 抗体 (C-11) Alexa Fluor® 488

sc-133225 AF488
200 µg/ml
$357.00

Histone Deacetylase 5 (HDAC5) 抗体 (C-11) Alexa Fluor® 546

sc-133225 AF546
200 µg/ml
$357.00

Histone Deacetylase 5 (HDAC5) 抗体 (C-11) Alexa Fluor® 594

sc-133225 AF594
200 µg/ml
$357.00

Histone Deacetylase 5 (HDAC5) 抗体 (C-11) Alexa Fluor® 647

sc-133225 AF647
200 µg/ml
$357.00

Histone Deacetylase 5 (HDAC5) 抗体 (C-11) Alexa Fluor® 680

sc-133225 AF680
200 µg/ml
$357.00

Histone Deacetylase 5 (HDAC5) 抗体 (C-11) Alexa Fluor® 790

sc-133225 AF790
200 µg/ml
$357.00

Does this antibody work with Immunocytochemistry

Asked by: Tayra
Thanks for your question. Yes, the antibody is recommended for detection of HDAC5 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA. Please contact Technical service for further support.
Answered by: SCBT Heidelberg Support
Date published: 2019-01-06

HDAC5のリン酸化抗体は取り扱っていますか？

Asked by: narumi
お問い合わせを頂きまして、誠にありがとうございました。HDAC5のリン酸化抗体は販売中止になっております。ご希望に添えず、申し訳ございません。その他の不 明点等がございましたら、またご連絡ください。 Thank you for your question. Unfortunately, p-HDAC5 antibody has been discontinued. Please feel free to contact me if I may be of further assistance.
Answered by: Technical Support 8
Date published: 2017-12-06

Is HDAC5 (C-11), sc-133225, available directly conjugated to HRP or fluorescent dyes?

Asked by: TinTin
Thank you for your question! Yes, sc-133225 is available conjugated to agarose (sc-133225 AC), HRP (sc-133225 HRP), phycoerythrin (sc-133225 PE), fluorescein (sc-133225 FITC), Alexa Fluor® 488 (sc-133225 AF488) and Alexa Fluor® 647 (sc-133225 AF647).
Answered by: Technical Support 6
Date published: 2017-01-10

Can this antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: TinTin
Thank you for your question. Yes, this HDAC5 antibody sc-133225 has been validated for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support 13
Date published: 2017-01-10
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Rated 5 out of 5 by from Great bandThis antibody works well for WB at 1: 500 dilution in cultured human podocytes.
Date published: 2019-11-14
Rated 5 out of 5 by from Great features!Work well in WB at 1:500.The band is quite clear,the quality is very good.
Date published: 2017-12-13
Rated 4 out of 5 by from works good for HeLa, HEK and HFFF cell linesI did HDAC5 IP with this antibody, it works very good on HEK and HeLa cells. It works even better on HFFF cells
Date published: 2017-11-12
Rated 5 out of 5 by from It's a very good antibody.Work well in WB at 1:500.this antibody brings great help to me.
Date published: 2017-09-02
Rated 4 out of 5 by from a wonderful wb antibody293T cells for wb，at 1:500，have a strong band，a good reasult
Date published: 2017-07-21
Rated 5 out of 5 by from Outstanding multiple applicationsStrong, clear western data; also superb IHC and IF staining
Date published: 2017-01-28
Rated 5 out of 5 by from Produces Excellent IHC(P) DataThe HDAC5 Antibody (C-11): sc-133225 provides great staining for IHC with formalin fixed, paraffin-embedded tissue sections. This antibody has also been cited multiple times for further verification.
Date published: 2017-01-11
Rated 3 out of 5 by from Reasonably potent and specific antibodyThis monoclonal antibody was independently tested in-house by Kinexus Bioinformatics Corporation and validated for use for Western blotting. There was medium-weak detection of the ~140-150 kDa target protein in mouse tissues (brain/testes/thymus/muscle mix). No major cross-reactivities were observed at 2 µg/ml antibody concentration with off-target proteins in the mouse tissues tested, except for a very strong ~35 kDa cross-reactive protein.
Date published: 2016-11-10
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Histone Deacetylase 5 (HDAC5) 抗体 (C-11) is rated 4.5 out of 5 by 12.
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