HDAC3 Antibody (1) recommended for detection of HDAC3 of mouse, rat and human origin by WB and ELISA

HDAC3 Antibody (1): sc-130319

HDAC3 Antibody (1) is rated 4.5 out of 5 by 2.
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Datasheets

    • HDAC3 Antibody (1) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • raised against recombinant HDAC3 of human origin
    • recommended for detection of HDAC3 of mouse, rat and human origin by WB and ELISA
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • See HDAC3 (A-3): sc-376957 for HDAC3 antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
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How much sample do you recommend loading on the gel for Western blot detection using HDAC3 (1): sc-130319?

Asked by: cjMara
We recommend loading 40–60 µg whole cell lysate, 10–20 µg nuclear extract, 5-10 µg transfected lysate, or 10–20 ng purified protein per lane. A link to our complete Western blotting protocol is provided here for your reference: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-03-01
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Rated 5 out of 5 by from Works well in human lung bronchial tissues in WB Works well in human lung bronchial tissues in WB. -SCBT Publication Review
Date published: 2015-01-13
Rated 4 out of 5 by from Good monoclonal antibody and showed strong Good monoclonal antibody and showed strong band in recombinant HDAC in WB. -SCBT QC
Date published: 2013-02-22
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