HDAC3 Antibody (A-3) recommended for detection of HDAC3 of mouse, rat, human and avian origin by WB, IP, IF and ELISA

HDAC3 Antibody (A-3): sc-376957

HDAC3 Antibody (A-3) is rated 4.8 out of 5 by 13.
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Datasheets

    • Anti-HDAC3 Antibody (A-3) is a mouse monoclonal IgG1 (kappa light chain) HDAC3 antibody provided at 200 µg/ml
    • specific for an epitope mapping between amino acids 2-29 at the N-terminus of HDAC3 of human origin
    • HDAC3 Antibody (A-3) is recommended for detection of HDAC3 of mouse, rat, human and avian origin by WB, IP, IF and ELISA; also reactive with additional species, including and equine, canine, bovine, porcine and avian
    • Anti-HDAC3 Antibody (A-3) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • TransCruz reagent for ChIP application (sc-376957 X, 200 µg/0.1 ml)
    • blocking peptide, sc-376957 P
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of HDAC3 (A-3): sc-376957.
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What is the recommended fixation method for IF with HDAC3 (A-3): sc-376957 monoclonal antibody?

Asked by: jenni
Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10ºC methanol and allowing to air dry. The full protocol can be viewed here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support 5
Date published: 2017-02-03
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Rated 4 out of 5 by from b3 works well Used a 1:300 concentration on nuclear mouse brain lysate for chemiluminescence Western Blot, it would be better if the concentration was higher than 1:300
Date published: 2018-04-23
Rated 5 out of 5 by from wonderful antibody! 1:200 diluted for wb of rat protein,the band is strong and special
Date published: 2018-04-21
Rated 5 out of 5 by from Works well! Band strong, specific and clear.........................................................................................
Date published: 2018-03-13
Rated 5 out of 5 by from It works well. Thanks for the trial sample. It works well.I'd like to recommend it.
Date published: 2017-11-27
Rated 4 out of 5 by from Good for western blot analysis of HDAC3 protein All lanes:HDAC3 抗体 (A-3): sc-376957 at 0.5ug/ml Lane1:SK-Hep1; Lane2:U2OS Lysates/protein at 80,000 cells per lane. I also used the antibody for immunofluorescence in SK-Hep1,but it doesn't work!
Date published: 2017-09-26
Rated 5 out of 5 by from good quality for WB This antibody works well and generates a clear signal at the predicted position on the western blot (1:500 dilution, 50ug of rat heart extract).
Date published: 2017-09-21
Rated 5 out of 5 by from western blot i got this sample , work very well western blot with 3T3 -l1 cell lysis.
Date published: 2017-08-14
Rated 5 out of 5 by from Work well for WB Great for WB, strong and clear band detected in 3T3L1 cell whole cell lysate.
Date published: 2017-08-09
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