HDAC11 Antibody (C-5) recommended for detection of HDAC11 of mouse, rat and human origin by WB, IP, IF and ELISA

HDAC11 Antibody (C-5): sc-390737

HDAC11 Antibody (C-5) is rated 4.4 out of 5 by 5.
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Datasheets

    • Anti-HDAC11 Antibody (C-5) is a mouse monoclonal IgG2b (kappa light chain) HDAC11 antibody provided at 200 µg/ml
    • raised against amino acids 1-300 mapping at the N-terminus of HDAC11 of human origin
    • Anti-HDAC11 Antibody (C-5) is recommended for detection of HDAC11 of mouse, rat and human origin by WB, IP, IF and ELISA
    • Anti-HDAC11 Antibody (C-5) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of HDAC11 (C-5): sc-390737.
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Using this antibody in WB(8% agarose gel), which one is the right band, about 37kDa or 50kDa?

Asked by: jm lee
Thank you for your question. The predicted molecular weight for HDAC11 is approximately 39 kDa. However, we would expect the observed molecular weight to be slightly higher than the predicted molecular weight. Unfortunately, we do not have any references to support a molecular weight as high as 50 kDa. We will review the data using this antibody on our website and make updates very shortly to remove any unsupportive data.
Answered by: Technical Support
Date published: 2017-08-30

If using the Li-Cor system, what is the recommended secondary for this antibody?

Asked by: TinTin
We recommend using one of our our exclusive Mouse IgG Binding Proteins (m-IgGκ) conjugated to CFL 680 or CFL 790. They are suitable for use with Near-Infrared (NIR) detection systems, such as LI-COR/Odyssey and other comparable systems. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Tech Service
Date published: 2017-01-30
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Rated 4 out of 5 by from Good antibody for WB. Test this antibody by WB using mouse lung cancer cell lysates and it works well although there are non-specific bands.
Date published: 2017-09-12
Rated 4 out of 5 by from a relative good antibody for WB This antibody generates a clear signal at the predicted position on the western blot (1:500 dilution, 40ug HeLa extract).
Date published: 2017-09-01
Rated 5 out of 5 by from Great HDAC11 monoclonal choice, strong WB bands! Highly specific strong band in Caki-1 whole cell lysate.
Date published: 2017-01-10
Rated 5 out of 5 by from Used this antibody in WB Used this antibody in WB, worked well, did not see any non specific bands, happy with purchase.
Date published: 2015-08-17
Rated 4 out of 5 by from Good specificity and signal strength by Western Good specificity and signal strength by Western in Caki-1 whole cell lysate. -SCBT QC
Date published: 2014-11-01
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