Hck Antibody (G-4): sc-166463
- Hck Antibody (G-4) is a mouse monoclonal IgG1 κ provided at 200 µg/ml
- raised against amino acids 1-85 of Hck of human origin
- Anti-Hck Antibody (G-4) is recommended for detection of Hck of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-Hck Antibody (G-4) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for Hck Antibody (G-4) for WB applications. These reagents are now offered in bundles with Hck Antibody (G-4) (see ordering information below).
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SEE ALSO...
Hck Antibody (G-4) is a mouse monoclonal IgG1 kappa light chain antibody that detects Hck in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). anti-Hck antibody (G-4) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Hck, a member of the Src family of non-receptor tyrosine kinases, plays a crucial role in hematopoietic signaling pathways, particularly in the regulation of immune cell functions. Hck is predominantly expressed in hematopoietic cells, especially granulocytes, where Hck is involved in processes such as cell proliferation, differentiation, and migration. Hck′s importance lies in its ability to modulate signaling cascades that govern immune responses, making Hck a vital player in the body′s defense mechanisms. Hck is located on chromosome 20q11-q12 and encodes a 505 amino acid protein, which is typically maintained in an inactive state and can be activated during cellular events like mitosis. Hck′s specific interactions with other proteins in the Src family, such as Lyn and Fyn, further underscore Hck′s significance in cellular signaling and potential implications in various diseases, including cancer and immune disorders.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Hck Antibody (G-4) References:
- Regulation of p73 by Hck through kinase-dependent and independent mechanisms. | Paliwal, P., et al. 2007. BMC Mol Biol. 8: 45. PMID: 17535448
- Hck contributes to bone homeostasis by controlling the recruitment of osteoclast precursors. | Vérollet, C., et al. 2013. FASEB J. 27: 3608-18. PMID: 23742809
- HCK is a survival determinant transactivated by mutated MYD88, and a direct target of ibrutinib. | Yang, G., et al. 2016. Blood. 127: 3237-52. PMID: 27143257
- Lck/Hck/Fgr-Mediated Tyrosine Phosphorylation Negatively Regulates TBK1 to Restrain Innate Antiviral Responses. | Liu, S., et al. 2017. Cell Host Microbe. 21: 754-768.e5. PMID: 28618271
- HIV Activates the Tyrosine Kinase Hck to Secrete ADAM Protease-Containing Extracellular Vesicles. | Lee, JH., et al. 2018. EBioMedicine. 28: 151-161. PMID: 29331674
- Identification of the SRC-family tyrosine kinase HCK as a therapeutic target in mantle cell lymphoma. | Lantermans, HC., et al. 2021. Leukemia. 35: 881-886. PMID: 32591642
- HCK can serve as novel prognostic biomarker and therapeutic target for Breast Cancer patients. | Zhu, X., et al. 2020. Int J Med Sci. 17: 2773-2789. PMID: 33162805
- miR-181b and miR-204 suppress the VSMC proliferation and migration by downregulation of HCK. | Ghasempour, G., et al. 2021. Microvasc Res. 136: 104172. PMID: 33894273
- HCK and ABAA: A Newly Designed Pipeline to Improve Fungi Metabarcoding Analysis. | Mlaga, KD., et al. 2021. Front Microbiol. 12: 640693. PMID: 34025601
- HCK is a Potential Prognostic Biomarker that Correlates with Immune Cell Infiltration in Acute Myeloid Leukemia. | Cheng, F., et al. 2022. Dis Markers. 2022: 3199589. PMID: 35280440
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Hck Antibody (G-4) | sc-166463 | 200 µg/ml | $316.00 | |||
Hck Antibody (G-4): m-IgG Fc BP-HRP Bundle | sc-527161 | 200 µg Ab; 10 µg BP | $354.00 | |||
Hck Antibody (G-4): m-IgG1 BP-HRP Bundle | sc-532534 | 200 µg Ab; 20 µg BP | $354.00 | |||
Hck Antibody (G-4) AC | sc-166463 AC | 500 µg/ml, 25% agarose | $416.00 | |||
Hck Antibody (G-4) HRP | sc-166463 HRP | 200 µg/ml | $316.00 | |||
Hck Antibody (G-4) FITC | sc-166463 FITC | 200 µg/ml | $330.00 | |||
Hck Antibody (G-4) PE | sc-166463 PE | 200 µg/ml | $343.00 | |||
Hck Antibody (G-4) Alexa Fluor® 488 | sc-166463 AF488 | 200 µg/ml | $357.00 | |||
Hck Antibody (G-4) Alexa Fluor® 546 | sc-166463 AF546 | 200 µg/ml | $357.00 | |||
Hck Antibody (G-4) Alexa Fluor® 594 | sc-166463 AF594 | 200 µg/ml | $357.00 | |||
Hck Antibody (G-4) Alexa Fluor® 647 | sc-166463 AF647 | 200 µg/ml | $357.00 | |||
Hck Antibody (G-4) Alexa Fluor® 680 | sc-166463 AF680 | 200 µg/ml | $357.00 | |||
Hck Antibody (G-4) Alexa Fluor® 790 | sc-166463 AF790 | 200 µg/ml | $357.00 |