Hck Antibody (3D12E10): sc-101428
- Hck Antibody (3D12E10) is a mouse monoclonal IgG1 κ Hck antibody, cited in 6 publications, provided at 200 µg/ml
- raised against recombinant Hck of human origin
- Hck Antibody (3D12E10) is recommended for detection of Hck of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
- Anti-Hck Antibody (3D12E10) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Hck (3D12E10): sc-101428.
- m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for Hck Antibody (3D12E10) for WB and IHC(P) applications. These reagents are now offered in bundles with Hck Antibody (3D12E10) (see ordering information below).
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Hck Antibody (3D12E10) is a mouse monoclonal IgG1 kappa light chain antibody that detects Hck protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). Anti-Hck antibody (3D12E10) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Hck, a member of the Src family of non-receptor tyrosine kinases, plays a crucial role in hematopoietic cell signaling, particularly in regulating immune responses and inflammation. Hck is predominantly expressed in hematopoietic cells, especially granulocytes, and is essential for processes such as cell proliferation, differentiation, and migration. Hck localizes in the cytoplasm and can move to the cell membrane upon activation, which is vital for signal transduction pathways. The human Hck gene is located on chromosome 20q11-q12 and encodes a 505 amino acid protein that modulates various cellular activities, making Hck a significant target for research in cancer and immune-related diseases.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Hck Antibody (3D12E10) References:
- Kinases of the Src family: structure and functions. | Tatosyan, AG. and Mizenina, OA. 2000. Biochemistry (Mosc). 65: 49-58. PMID: 10702640
- Src kinase-mediated signaling in leukocytes. | Korade-Mirnics, Z. and Corey, SJ. 2000. J Leukoc Biol. 68: 603-13. PMID: 11073097
- Selected glimpses into the activation and function of Src kinase. | Bjorge, JD., et al. 2000. Oncogene. 19: 5620-35. PMID: 11114743
- SRC family kinases mediate epithelial Na+ channel inhibition by endothelin. | Gilmore, ES., et al. 2001. J Biol Chem. 276: 42610-7. PMID: 11560932
- Hck inhibitors as potential therapeutic agents in cancer and HIV infection. | Musumeci, F., et al. 2015. Curr Med Chem. 22: 1540-64. PMID: 25666803
- Novel protein-tyrosine kinase gene (hck) preferentially expressed in cells of hematopoietic origin. | Ziegler, SF., et al. 1987. Mol Cell Biol. 7: 2276-85. PMID: 3453117
- Identification of a human gene (HCK) that encodes a protein-tyrosine kinase and is expressed in hemopoietic cells. | Quintrell, N., et al. 1987. Mol Cell Biol. 7: 2267-75. PMID: 3496523
- HCK induces macrophage activation to promote renal inflammation and fibrosis via suppression of autophagy. | Chen, M., et al. 2023. Nat Commun. 14: 4297. PMID: 37463911
- The dual HCK/BTK inhibitor KIN-8194 impairs growth and integrin-mediated adhesion of BTKi-resistant mantle cell lymphoma. | Lantermans, HC., et al. 2024. Leukemia. 38: 1570-1580. PMID: 38454120
- Abnormal HCK/glutamine/autophagy axis promotes endometriosis development by impairing macrophage phagocytosis. | Lei, ST., et al. 2024. Cell Prolif. 57: e13702. PMID: 38956970
- Genetic organization of human proto-oncogenes. | Sakaguchi, AY. 1983. Prog Clin Biol Res. 119: 93-103. PMID: 6191340
- Insights into Src kinase functions: structural comparisons. | Williams, JC., et al. 1998. Trends Biochem Sci. 23: 179-84. PMID: 9612082
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Hck Antibody (3D12E10) | sc-101428 | 200 µg/ml | $316.00 | |||
Hck Antibody (3D12E10): m-IgG Fc BP-HRP Bundle | sc-527071 | 200 µg Ab; 10 µg BP | $354.00 | |||
Hck Antibody (3D12E10): m-IgG1 BP-HRP Bundle | sc-532444 | 200 µg Ab; 20 µg BP | $354.00 | |||
Hck Antibody (3D12E10) AC | sc-101428 AC | 500 µg/ml, 25% agarose | $416.00 | |||
Hck Antibody (3D12E10) HRP | sc-101428 HRP | 200 µg/ml | $316.00 | |||
Hck Antibody (3D12E10) FITC | sc-101428 FITC | 200 µg/ml | $330.00 | |||
Hck Antibody (3D12E10) PE | sc-101428 PE | 200 µg/ml | $343.00 | |||
Hck Antibody (3D12E10) Alexa Fluor® 488 | sc-101428 AF488 | 200 µg/ml | $357.00 | |||
Hck Antibody (3D12E10) Alexa Fluor® 546 | sc-101428 AF546 | 200 µg/ml | $357.00 | |||
Hck Antibody (3D12E10) Alexa Fluor® 594 | sc-101428 AF594 | 200 µg/ml | $357.00 | |||
Hck Antibody (3D12E10) Alexa Fluor® 647 | sc-101428 AF647 | 200 µg/ml | $357.00 | |||
Hck Antibody (3D12E10) Alexa Fluor® 680 | sc-101428 AF680 | 200 µg/ml | $357.00 | |||
Hck Antibody (3D12E10) Alexa Fluor® 790 | sc-101428 AF790 | 200 µg/ml | $357.00 |