Hck Antibody (A-8): sc-514855

Hck Antibody (A-8) is a mouse monoclonal IgG2b antibody that detects Hck in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-Hck antibody (A-8) specifically targets human hemopoietic cell kinase (Hck), a crucial non-receptor tyrosine kinase that plays a significant role in the signaling pathways of hematopoietic cells, particularly in granulocytes. Hck (A-8) antibody recognizes a protein of 505 amino acids, primarily localized in hematopoietic tissues, where Hck is essential for regulating various cellular processes, including cell proliferation, differentiation, and migration. Hck (A-8) monoclonal antibody targets a protein vital for immune responses, as Hck influences the activation and movement of immune cells during inflammatory processes. Additionally, Hck undergoes post-translational modifications, such as phosphorylation, which can modulate activity and interactions with other signaling molecules, thereby impacting cellular signaling cascades. Understanding Hck dynamics and regulatory mechanisms is important for elucidating contributions to hematopoietic cell function and potential implications in diseases such as leukemia and other hematological disorders.

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Hck Antibody (A-8) References:

1. Characteristic expression of Hck in human B-cell precursors.  |  Taguchi, T., et al. 2000. Exp Hematol. 28: 55-64. PMID: 10658677
2. Association of Hck genetic polymorphisms with gene expression and COPD.  |  Zhang, X., et al. 2007. Hum Genet. 120: 681-90. PMID: 17024369
3. Tyrosine phosphorylation of Wiskott-Aldrich syndrome protein (WASP) by Hck regulates macrophage function.  |  Park, H., et al. 2014. J Biol Chem. 289: 7897-906. PMID: 24482227
4. Subtle Dynamic Changes Accompany Hck Activation by HIV-1 Nef and are Reversed by an Antiretroviral Kinase Inhibitor.  |  Wales, TE., et al. 2015. Biochemistry. 54: 6382-91. PMID: 26440750
5. An essential pathway links FLT3-ITD, HCK and CDK6 in acute myeloid leukemia.  |  Lopez, S., et al. 2016. Oncotarget. 7: 51163-51173. PMID: 27323399
6. Phosphorylated and non-phosphorylated HCK kinase domains produced by cell-free protein expression.  |  Katsura, K., et al. 2018. Protein Expr Purif. 150: 92-99. PMID: 29793032
7. Discovery of Non-peptide Small Molecule Allosteric Modulators of the Src-family Kinase, Hck.  |  Dorman, HR., et al. 2019. Front Chem. 7: 822. PMID: 31850311
8. Hematopoietic Cell Kinase (HCK) Is a Player of the Crosstalk Between Hematopoietic Cells and Bone Marrow Niche Through CXCL12/CXCR4 Axis.  |  Roversi, FM., et al. 2021. Front Cell Dev Biol. 9: 634044. PMID: 33842460
9. Hck expression correlates with granulocyte-macrophage colony-stimulating factor-induced proliferation in HL-60 cells.  |  Linnekin, D., et al. 1994. Blood. 84: 94-103. PMID: 8018933
10. Deficiency of the Hck and Src tyrosine kinases results in extreme levels of extramedullary hematopoiesis.  |  Lowell, CA., et al. 1996. Blood. 87: 1780-92. PMID: 8634424

The preferred Hck antibody is Hck Antibody (3D12E10): sc-101428.