HBO1 Antibody (G-2): sc-398346

HBO1 Antibody (G-2) is a mouse monoclonal IgG2b kappa light chain antibody that detects HBO1 of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). HBO1 (G-2) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. HBO1, or histone acetyltransferase binding to ORC, is a member of the MYST family of histone acetyltransferases, which play a crucial role in regulating transcription through chromatin modification. HBO1 is primarily located in the nucleus and is highly expressed in human testis, where HBO1 is involved in histone acetylation, thereby influencing gene expression and DNA replication. HBO1 interacts with ORC1, the largest subunit of the origin recognition complex, which is essential for initiating DNA replication. By mediating histone acetylation, HBO1 not only facilitates gene transcription but also represses androgen receptor-mediated transcription, indicating a potential role in regulating gene expression in both normal physiology and prostate cancer. HBO1′s ability to modulate these critical processes underscores its importance in cellular function and highlights its relevance as a target for research in cancer biology and transcriptional regulation.

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HBO1 Antibody (G-2) References:

1. Histone acetyltransferase HBO1 interacts with the ORC1 subunit of the human initiator protein.  |  Iizuka, M. and Stillman, B. 1999. J Biol Chem. 274: 23027-34. PMID: 10438470
2. Androgen receptor interacts with a novel MYST protein, HBO1.  |  Sharma, M., et al. 2000. J Biol Chem. 275: 35200-8. PMID: 10930412
3. Cyclin-dependent kinase 11(p58) interacts with HBO1 and enhances its histone acetyltransferase activity.  |  Zong, H., et al. 2005. FEBS Lett. 579: 3579-88. PMID: 15963510
4. Regulation of replication licensing by acetyltransferase Hbo1.  |  Iizuka, M., et al. 2006. Mol Cell Biol. 26: 1098-108. PMID: 16428461
5. Hbo1 Links p53-dependent stress signaling to DNA replication licensing.  |  Iizuka, M., et al. 2008. Mol Cell Biol. 28: 140-53. PMID: 17954561
6. Role of Jade-1 in the histone acetyltransferase (HAT) HBO1 complex.  |  Foy, RL., et al. 2008. J Biol Chem. 283: 28817-26. PMID: 18684714
7. HBO1 histone acetylase is a coactivator of the replication licensing factor Cdt1.  |  Miotto, B. and Struhl, K. 2008. Genes Dev. 22: 2633-8. PMID: 18832067
8. The histone acetyltransferase HBO1 functions as a novel oncogenic gene in osteosarcoma.  |  Gao, YY., et al. 2021. Theranostics. 11: 4599-4615. PMID: 33754016
9. HBO1 is a versatile histone acyltransferase critical for promoter histone acylations.  |  Xiao, Y., et al. 2021. Nucleic Acids Res. 49: 8037-8059. PMID: 34259319
10. Histone acetylation by HBO1 (KAT7) activates Wnt/β-catenin signaling to promote leukemogenesis in B-cell acute lymphoblastic leukemia.  |  Wang, H., et al. 2023. Cell Death Dis. 14: 498. PMID: 37542030
11. Guiding the HBO1 complex function through the JADE subunit.  |  Gaurav, N., et al. 2024. Nat Struct Mol Biol. 31: 1039-1049. PMID: 38448574
12. HBO1 catalyzes lysine lactylation and mediates histone H3K9la to regulate gene transcription.  |  Niu, Z., et al. 2024. Nat Commun. 15: 3561. PMID: 38670996

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-HBO1 Antibody (G-2) has 2 citations in a variety of scientific publications.

Primary antibodies like Anti-HBO1 Antibody (G-2) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

HBO1 Antibody (G-2) Specifications

Alternate Product Name: HBO1

Product Clone Name: G-2

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 20-47

Epitope Target: HBO1

Epitope Species: human

Product Reactivity/Detection: HBO1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: equine, canine, bovine and porcine

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 2 citations in various scientific publications

Available Conjugates/Forms for HBO1 Antibody (G-2): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin