HBO1 Antibody (G-2) recommended for detection of HBO1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

HBO1 Antibody (G-2): sc-398346

HBO1 Antibody (G-2) is rated 4.2 out of 5 by 5.
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Datasheets

    • Anti-HBO1 Antibody (G-2) is a mouse monoclonal IgG2b (kappa light chain) HBO1 antibody provided at 200 µg/ml
    • specific for an epitope mapping between amino acids 20-47 near the N-terminus of HBO1 of human origin
    • Anti-HBO1 Antibody (G-2) is recommended for detection of HBO1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including and equine, canine, bovine and porcine
    • Anti-HBO1 Antibody (G-2) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • blocking peptide, sc-398346 P
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of HBO1 (G-2): sc-398346.

What application is the blocking peptide sc-398346 P appropriate for?

Asked by: Cweed
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-27
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Rated 4 out of 5 by from 结果还行 这个都是一个月之前申请的抗体,当时申请了几个,这是其中一个,效果还行,结果算是比较满意,如果处理一下,应该会有更好的结果吧
Date published: 2017-12-11
Rated 3 out of 5 by from 没有曝出条带 293T细胞及HL-1细胞蛋白裂解液上样都没有曝出条带,不知是否是裂解条件不利于释放核蛋白导致的,准备孵H3内参再试试看。
Date published: 2017-04-26
Rated 4 out of 5 by from Good I tried the sample and it worked well for me, good signal.
Date published: 2017-03-16
Rated 5 out of 5 by from Nice Western Blot data of HBO1 expression in non Nice Western Blot data of HBO1 expression in non-transfected 293T, human HBO1 transfected 293T and HeLa whole cell lysates and A-673, HeLa and A-431 nuclear extracts. -SCBT QC
Date published: 2015-03-21
Rated 5 out of 5 by from Produced positive Western Blot data of HBO1 Produced positive Western Blot data of HBO1 expression in non-transfected and mouse HBO1 transfected 293T whole cell lysates. -SCBT QC
Date published: 2013-11-25
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