HAX-1 Antibody (B-11): sc-166845

HAX-1 Antibody (B-11) is a mouse monoclonal IgG1 kappa light chain antibody that detects HAX-1 of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). HAX-1 antibody (B-11) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. HAX-1, also known as HS1-associated protein X-1, plays a crucial role in cellular processes, particularly in the regulation of apoptosis and signal transduction in B cells. HAX-1 localizes primarily in the mitochondria, but is also found in the endoplasmic reticulum and the nuclear envelope, which is significant for involvement in cellular stress responses and survival pathways. HAX-1 interaction with various proteins, such as Polycystin-2, Cortactin, and the K15 protein from Kaposi′s sarcoma-associated herpesvirus, highlights importance in both normal cellular functions and disease states. HAX-1′s co-localization with these proteins in the endoplasmic reticulum and mitochondria suggests a complex role in cellular signaling and structural integrity. HAX-1 gene expression across different tissues further emphasizes a fundamental role in maintaining cellular homeostasis and responding to stress signals.

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HAX-1 Antibody (B-11) References:

1. The polycystic kidney disease protein PKD2 interacts with Hax-1, a protein associated with the actin cytoskeleton.  |  Gallagher, AR., et al. 2000. Proc Natl Acad Sci U S A. 97: 4017-22. PMID: 10760273
2. Interaction of Epstein-Barr virus nuclear antigen leader protein (EBNA-LP) with HS1-associated protein X-1: implication of cytoplasmic function of EBNA-LP.  |  Kawaguchi, Y., et al. 2000. J Virol. 74: 10104-11. PMID: 11024139
3. Identification of human epidermal differentiation complex (EDC)-encoded genes by subtractive hybridization of entire YACs to a gridded keratinocyte cDNA library.  |  Marenholz, I., et al. 2001. Genome Res. 11: 341-55. PMID: 11230159
4. Epstein-Barr virus nuclear antigen 5 interacts with HAX-1, a possible component of the B-cell receptor signalling pathway.  |  Dufva, M., et al. 2001. J Gen Virol. 82: 1581-1587. PMID: 11413368
5. Evidence that HAX-1 is an interleukin-1 alpha N-terminal binding protein.  |  Yin, H., et al. 2001. Cytokine. 15: 122-37. PMID: 11554782
6. K15 protein of Kaposi's sarcoma-associated herpesvirus is latently expressed and binds to HAX-1, a protein with antiapoptotic function.  |  Sharp, TV., et al. 2002. J Virol. 76: 802-16. PMID: 11752170
7. HAX-1 represses postmitochondrial caspase-9 activation and cell death during hypoxia-reoxygenation.  |  Shaw, J. and Kirshenbaum, LA. 2006. Circ Res. 99: 336-8. PMID: 16917098
8. Hax-1: a regulator of calcium signaling and apoptosis progression with multiple roles in human disease.  |  Simmen, T. 2011. Expert Opin Ther Targets. 15: 741-51. PMID: 21391832
9. Neuroprotective roles of HAX-1 in ischemic neuronal injury.  |  Sui, X., et al. 2021. Exp Neurol. 339: 113642. PMID: 33600816
10. Anti-apoptotic HAX-1 suppresses cell apoptosis by promoting c-Abl kinase-involved ROS clearance.  |  Dong, Q., et al. 2022. Cell Death Dis. 13: 298. PMID: 35379774
11. Hax-1 Regulates Radiation-Induced Mitochondrial-Dependent Apoptosis of Uveal Melanoma Cells through PI3K/AKT/eNOS Pathway.  |  Wang, S., et al. 2022. J Oncol. 2022: 2956888. PMID: 35602302
12. HAX-1, a novel intracellular protein, localized on mitochondria, directly associates with HS1, a substrate of Src family tyrosine kinases.  |  Suzuki, Y., et al. 1997. J Immunol. 158: 2736-44. PMID: 9058808

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-HAX-1 Antibody (B-11) has 9 citations in a variety of scientific publications.

Primary antibodies like Anti-HAX-1 Antibody (B-11) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

HAX-1 Antibody (B-11) Specifications

Alternate Product Name: HAX-1

Product Clone Name: B-11

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 1-279

Epitope Target: HAX-1

Epitope Species: human

Product Reactivity/Detection: HAX-1

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 9 citations in various scientific publications

Available Conjugates/Forms for HAX-1 Antibody (B-11): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin