HAS2 Antibody (C-5): sc-365263

HAS2 Antibody (C-5) is a mouse monoclonal IgM antibody that detects hyaluronan synthase 2 (HAS2) in mouse, rat, and human samples through western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). HAS2 Antibody (C-5) is available in a non-conjugated form, allowing for versatile experimental use. HAS2 is one of three hyaluronan synthase proteins, alongside HAS1 and HAS3, which are crucial for synthesizing hyaluronic acid (HA), a vital component of the extracellular matrix in vertebrates. HA is a high molecular weight linear polysaccharide composed of alternating glucuronic acid and N-acetylglucosamine residues, linked by β-1,3 and β-1,4 glycosidic bonds. The unique structure of HA allows water retention and provides hydration and lubrication in tissues, making HA essential for maintaining cartilage and other connective tissues. The differential expression of HAS1, HAS2, and HAS3 during development indicates distinct roles in HA matrix formation, with HAS2 being predominantly expressed in chondrocytes and cartilage, highlighting HAS2′s importance in skeletal development and joint health. The human HAS2 gene is located on chromosome 8q24.12, and HAS2 regulation is influenced by various stimuli, further emphasizing HAS2′s functional significance in physiological and pathological processes.

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HAS2 Antibody (C-5) References:

1. Three isoforms of mammalian hyaluronan synthases have distinct enzymatic properties.  |  Itano, N., et al. 1999. J Biol Chem. 274: 25085-92. PMID: 10455188
2. Expression of human hyaluronan synthases in response to external stimuli.  |  Jacobson, A., et al. 2000. Biochem J. 348 Pt 1: 29-35. PMID: 10794710
3. Differential regulation and expression of hyaluronan synthases in human articular chondrocytes, synovial cells and osteosarcoma cells.  |  Recklies, AD., et al. 2001. Biochem J. 354: 17-24. PMID: 11171074
4. Regulation of hyaluronan synthase gene expression in human periodontal ligament cells by tumour necrosis factor-alpha, interleukin-1beta and interferon-gamma.  |  Ijuin, C., et al. 2001. Arch Oral Biol. 46: 767-72. PMID: 11389868
5. Abnormal accumulation of hyaluronan matrix diminishes contact inhibition of cell growth and promotes cell migration.  |  Itano, N., et al. 2002. Proc Natl Acad Sci U S A. 99: 3609-14. PMID: 11891291
6. HAS2 and CD44 in breast tumorigenesis.  |  Heldin, P., et al. 2014. Adv Cancer Res. 123: 211-29. PMID: 25081531
7. Atherosclerosis-associated endothelial cell apoptosis by miRNA let7-b-mediated downregulation of HAS-2.  |  Zhu, L., et al. 2020. J Cell Biochem. 121: 3961-3972. PMID: 31736114
8. The natural antisense transcript HAS2-AS1 regulates breast cancer cells aggressiveness independently from hyaluronan metabolism.  |  Parnigoni, A., et al. 2022. Matrix Biol. 109: 140-161. PMID: 35395387
9. Downregulation of HAS‑2 regulates the chondrocyte cytoskeleton and induces cartilage degeneration by activating the RhoA/ROCK signaling pathway.  |  Yang, J., et al. 2023. Int J Mol Med. 52: PMID: 37232339
10. Increased hyaluronan by naked mole-rat Has2 improves healthspan in mice.  |  Zhang, Z., et al. 2023. Nature. 621: 196-205. PMID: 37612507
11. KIAA1429/VIRMA promotes breast cancer progression by m6 A-dependent cytosolic HAS2 stabilization.  |  Li, N., et al. 2023. EMBO Rep. 24: e55506. PMID: 37705505
12. HAS2 facilitates glioma cell malignancy and suppresses ferroptosis in an FZD7-dependent manner.  |  Liu, Z., et al. 2024. Cancer Sci. 115: 2602-2616. PMID: 38816349

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-HAS2 Antibody (C-5) has 19 citations in a variety of scientific publications.

Primary antibodies like Anti-HAS2 Antibody (C-5) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

HAS2 Antibody (C-5) Specifications

Alternate Product Name: hyaluronan synthase 2 (HAS2)

Product Clone Name: C-5

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgM kappa light chain

Amino Acids:

Epitope Target: HAS2

Epitope Species: human

Product Reactivity/Detection: HAS2

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgM kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 19 citations in various scientific publications

Available Conjugates/Forms for HAS2 Antibody (C-5): P: Blocking Peptide

The preferred HAS2 antibody is HAS2 Antibody (A-7): sc-514737.