H-Ras Antibody (238): sc-34

H-Ras Antibody (238) is a rat monoclonal IgG2a antibody that specifically targets the H-Ras protein, a member of the Ras family of small GTPases. H-Ras, along with K-Ras and N-Ras, plays a crucial role in regulating signal transduction pathways that control various essential cellular processes, such as cell growth, differentiation, survival, and migration. H-Ras protein functions as a molecular switch by cycling between an active GTP-bound state and an inactive GDP-bound state. Activation of H-Ras is mediated by guanine nucleotide exchange factors (GEFs), while GTPase-activating proteins (GAPs) facilitate inactivation by promoting GTP hydrolysis. This regulation is important for maintaining proper cellular function and preventing uncontrolled cell proliferation, which can lead to oncogenesis. Aberrant activation of H-Ras due to mutations is frequently associated with cancer, as H-Ras deregulates critical pathways, including the MAP kinase and PI3K/AKT pathways, that influence cell division and apoptosis. H-Ras protein is also associated with specific post-translational modifications such as prenylation, which aids in membrane localization, a crucial aspect of signaling capabilities. H-Ras (238) monoclonal antibody reacts with human, mouse, and rat samples and is effective in applications such as western blotting (WB), immunoprecipitation (IP), and immunofluorescence (IF).

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H-Ras Antibody (238) References:

1. H-Ras signals to cytoskeletal machinery in induction of integrin-mediated adhesion of T cells.  |  Tanaka, Y., et al. 1999. J Immunol. 163: 6209-16. PMID: 10570313
2. Targeted genomic disruption of H-ras and N-ras, individually or in combination, reveals the dispensability of both loci for mouse growth and development.  |  Esteban, LM., et al. 2001. Mol Cell Biol. 21: 1444-52. PMID: 11238881
3. Dual role of H-Ras in regulation of lymphocyte function antigen-1 activity by stromal cell-derived factor-1alpha: implications for leukocyte transmigration.  |  Weber, KS., et al. 2001. Mol Biol Cell. 12: 3074-86. PMID: 11598192
4. Activated forms of H-RAS and K-RAS differentially regulate membrane association of PI3K, PDK-1, and AKT and the effect of therapeutic kinase inhibitors on cell survival.  |  Carón, RW., et al. 2005. Mol Cancer Ther. 4: 257-70. PMID: 15713897
5. Modulation of LFA-1 surface antigen expression by activated H-ras oncogene in EBV-infected human B-lymphoblast cells.  |  Endo, K., et al. 1991. Oncogene. 6: 1391-6. PMID: 1653411
6. A proteomic approach for dissecting H-Ras signaling networks in NIH/3T3 mouse embryonic fibroblast cells.  |  Park, JW., et al. 2006. Proteomics. 6: 2433-43. PMID: 16612794
7. Transformation by v-H-ras does not restore proliferation of a set of temperature-sensitive cell-cycle mutants of rat 3Y1 fibroblasts.  |  Shimura, H., et al. 1990. Cell Struct Funct. 15: 211-9. PMID: 2257639
8. Sodium nitroprusside induces H-Ras depalmitoylation and alters the cellular response to hypoxia in differentiated and undifferentiated PC12 cells.  |  Goloshvili, G., et al. 2019. Cell Biochem Funct. 37: 545-552. PMID: 31429100
9. In vivo effects of activated H-ras oncogene expressed in the liver and in urogenital tissues.  |  Gilbert, E., et al. 1997. Int J Cancer. 73: 749-56. PMID: 9398057
10. The role of ligand binding in the kinetic folding mechanism of human p21(H-ras) protein.  |  Zhang, J. and Matthews, CR. 1998. Biochemistry. 37: 14891-9. PMID: 9778365

The preferred pan Ras antibody is pan Ras Antibody (F132): sc-32.