h-prune Antibody (F-5): sc-393318
- h-prune Antibody (F-5) is a mouse monoclonal IgG1 κ h-prune antibody, cited in 1 publications, provided at 200 µg/ml
- raised against amino acids 197-249 mapping within an internal region of h-prune of human origin
- h-prune Antibody (F-5) is recommended for detection of h-prune of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-h-prune Antibody (F-5) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for h-prune Antibody (F-5) for WB applications. These reagents are now offered in bundles with h-prune Antibody (F-5) (see ordering information below).
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h-prune Antibody (F-5) is a mouse monoclonal IgG1 kappa light chain antibody that detects h-prune protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). anti-h-prune antibody (F-5) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. h-prune protein, also known as DRES17 (Drosophila-related expressed sequence 17), is a 453 amino acid protein that plays a crucial role in cellular processes by localizing to the cytoplasm, nucleus, and cell junctions. h-prune belongs to the prune subfamily of phosphatase class C proteins and is expressed ubiquitously throughout various tissues. Functionally, h-prune acts as a phosphodiesterase, utilizing manganese as a cofactor to catalyze the conversion of diphosphates into two free phosphates, which is vital for regulating cellular signaling pathways that influence cell proliferation and motility. h-prune is overexpressed in aggressive sarcoma subtypes, such as leiomyosarcomas and malignant fibrous histiocytomas, indicating potential involvement in tumor development and metastasis. Multiple isoforms of h-prune exist due to alternative splicing events, highlighting the complexity of regulation and function in different cellular contexts.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
h-prune Antibody (F-5) References:
- Sequencing analysis of forty-eight human image cDNA clones similar to Drosophila mutant protein. | Volorio, S., et al. 1998. DNA Seq. 9: 307-15. PMID: 10524757
- Evidence for interaction between human PRUNE and nm23-H1 NDPKinase. | Reymond, A., et al. 1999. Oncogene. 18: 7244-52. PMID: 10602478
- Amplification and overexpression of PRUNE in human sarcomas and breast carcinomas-a possible mechanism for altering the nm23-H1 activity. | Forus, A., et al. 2001. Oncogene. 20: 6881-90. PMID: 11687967
- Overexpression of h-prune in breast cancer is correlated with advanced disease status. | Zollo, M., et al. 2005. Clin Cancer Res. 11: 199-205. PMID: 15671547
- Glycogen synthase kinase 3 and h-prune regulate cell migration by modulating focal adhesions. | Kobayashi, T., et al. 2006. Mol Cell Biol. 26: 898-911. PMID: 16428445
- Domain mapping on the human metastasis regulator protein h-Prune reveals a C-terminal dimerization domain. | Middelhaufe, S., et al. 2007. Biochem J. 407: 199-205. PMID: 17655525
- h-Prune is an independent prognostic marker for survival in esophageal squamous cell carcinoma. | Noguchi, T., et al. 2009. Ann Surg Oncol. 16: 1390-6. PMID: 17896143
- Understanding h-prune biology in the fight against cancer. | Marino, N. and Zollo, M. 2007. Clin Exp Metastasis. 24: 637-45. PMID: 17952613
- Human metastasis regulator protein H-prune is a short-chain exopolyphosphatase. | Tammenkoski, M., et al. 2008. Biochemistry. 47: 9707-13. PMID: 18700747
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
h-prune Antibody (F-5) | sc-393318 | 200 µg/ml | $322.00 | |||
h-prune Antibody (F-5): m-IgG Fc BP-HRP Bundle | sc-530428 | 200 µg Ab; 10 µg BP | $361.00 | |||
h-prune Antibody (F-5): m-IgGκ BP-HRP Bundle | sc-523902 | 200 µg Ab, 40 µg BP | $361.00 | |||
h-prune Antibody (F-5): m-IgG1 BP-HRP Bundle | sc-544047 | 200 µg Ab; 20 µg BP | $361.00 | |||
h-prune Antibody (F-5) AC | sc-393318 AC | 500 µg/ml, 25% agarose | $424.00 | |||
h-prune Antibody (F-5) HRP | sc-393318 HRP | 200 µg/ml | $322.00 | |||
h-prune Antibody (F-5) FITC | sc-393318 FITC | 200 µg/ml | $336.00 | |||
h-prune Antibody (F-5) PE | sc-393318 PE | 200 µg/ml | $349.00 | |||
h-prune Antibody (F-5) Alexa Fluor® 488 | sc-393318 AF488 | 200 µg/ml | $364.00 | |||
h-prune Antibody (F-5) Alexa Fluor® 546 | sc-393318 AF546 | 200 µg/ml | $364.00 | |||
h-prune Antibody (F-5) Alexa Fluor® 594 | sc-393318 AF594 | 200 µg/ml | $364.00 | |||
h-prune Antibody (F-5) Alexa Fluor® 647 | sc-393318 AF647 | 200 µg/ml | $364.00 | |||
h-prune Antibody (F-5) Alexa Fluor® 680 | sc-393318 AF680 | 200 µg/ml | $364.00 | |||
h-prune Antibody (F-5) Alexa Fluor® 790 | sc-393318 AF790 | 200 µg/ml | $364.00 |