H⁺/K⁺ ATPase β Antibody (C-4): sc-374094

H+/K+ ATPase β Antibody (C-4) is a mouse monoclonal IgG2b kappa light chain antibody that detects H+/K+ ATPase beta protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). Anti-H+/K+ ATPase beta antibody (C-4) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. H+/K+ ATPase beta protein, also known as ATP4B or ATP6B, is a crucial component of the gastric H+/K+ ATPase heterodimer, which consists of an α and a β subunit that work together to transport protons across plasma membranes. This single-pass type II membrane protein, composed of 291 amino acids, plays a vital role in gastric acid secretion by catalyzing ATP hydrolysis while facilitating hydrogen and potassium ion exchange. Proper functioning of H+/K+ ATPase beta is essential for maintaining stomach acidity, which is necessary for digestion and digestive enzyme activation. Additionally, the gene encoding H+/K+ ATPase beta is located on human chromosome 13, a region containing over 400 genes, including those associated with critical health conditions. Understanding H+/K+ ATPase beta interactions and functions can provide insights into various gastrointestinal disorders and potential therapeutic targets.

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H⁺/K⁺ ATPase β Antibody (C-4) References:

1. The roles of carbohydrate chains of the beta-subunit on the functional expression of gastric H(+),K(+)-ATPase.  |  Asano, S., et al. 2000. J Biol Chem. 275: 8324-30. PMID: 10722662
2. Mapping of the gene encoding the beta-subunit of H+,K(+)-ATPase to human chromosome 13q34 by fluorescence in situ hybridization.  |  Song, I., et al. 1992. Genomics. 14: 1114-5. PMID: 1335958
3. Molecular and cellular regulation of the gastric proton pump.  |  Asano, S., et al. 2004. Biol Pharm Bull. 27: 1-12. PMID: 14743830
4. Transcriptome analysis of human gastric cancer.  |  Oh, JH., et al. 2005. Mamm Genome. 16: 942-54. PMID: 16341674
5. cDNA cloning of the beta-subunit of the human gastric H,K-ATPase.  |  Ma, JY., et al. 1991. Biochem Biophys Res Commun. 180: 39-45. PMID: 1656976
6. Human gastric (H+ + K+)-ATPase gene. Similarity to (Na+ + K+)-ATPase genes in exon/intron organization but difference in control region.  |  Maeda, M., et al. 1990. J Biol Chem. 265: 9027-32. PMID: 2160952
7. Luminescent Immunoprecipitation System (LIPS) for Detection of Autoantibodies Against ATP4A and ATP4B Subunits of Gastric Proton Pump H+,K+-ATPase in Atrophic Body Gastritis Patients.  |  Lahner, E., et al. 2017. Clin Transl Gastroenterol. 8: e215. PMID: 28102858
8. Silencing of ATP4B of ATPase H+/K+ Transporting Beta Subunit by Intragenic Epigenetic Alteration in Human Gastric Cancer Cells.  |  Lin, S., et al. 2017. Oncol Res. 25: 317-329. PMID: 28281974
9. Autoantibodies Toward ATP4A and ATP4B Subunits of Gastric Proton Pump H+,K+-ATPase Are Reliable Serological Pre-endoscopic Markers of Corpus Atrophic Gastritis.  |  Lahner, E., et al. 2020. Clin Transl Gastroenterol. 11: e00240. PMID: 33031196
10. Tumor suppressor ATP4B serve as a promising biomarker for worsening of gastric atrophy and poor differentiation.  |  Pan, Y., et al. 2021. Gastric Cancer. 24: 314-326. PMID: 33111209
11. Renal expression of the gene encoding the gastric H(+)-K(+)-ATPase beta-subunit.  |  Callaghan, JM., et al. 1995. Am J Physiol. 268: F363-74. PMID: 7900835
12. Proton pump inhibitors and acid-related diseases.  |  Sachs, G. 1997. Pharmacotherapy. 17: 22-37. PMID: 9017763

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-H⁺/K⁺ ATPase β Antibody (C-4) has 9 citations in a variety of scientific publications.

Primary antibodies like Anti-H⁺/K⁺ ATPase β Antibody (C-4) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

H⁺/K⁺ ATPase β Antibody (C-4) Specifications

Alternate Product Name: H+/K+ ATPase β

Product Clone Name: C-4

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids:

Epitope Target: H⁺/K⁺ ATPase β

Epitope Species: human

Product Reactivity/Detection: H⁺/K⁺ ATPase β

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 9 citations in various scientific publications

Available Conjugates/Forms for H⁺/K⁺ ATPase β Antibody (C-4): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin