GW182 Antibody (A-8): sc-377006
- GW182 Antibody (A-8) is a mouse monoclonal IgG2a κ, cited in 4 publications, provided at 200 µg/ml
- raised against amino acids 1856-1925 mapping near the C-terminus of GW182 of human origin
- recommended for detection of GW182 isoforms 1-4 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including canine
- See GW182 (A-6): sc-374458 for GW182 antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
- m-IgG Fc BP-HRP, m-IgG2a BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for GW182 Antibody (A-8) for WB and IHC(P) applications. These reagents are now offered in bundles with GW182 Antibody (A-8) (see ordering information below).
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GW182 Antibody (A-8) is a mouse monoclonal IgG2a antibody that detects GW182 in mouse, rat, and human samples through various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). GW182 plays a pivotal role in the formation and function of GW bodies (GWBs), which are essential for the regulation of mRNA stability and degradation. These structures serve as critical hubs for microRNA (miRNA) activity, and proper functioning of GW182 is vital for maintaining GWB integrity. Disruption of GW182 can lead to impaired silencing of small interfering RNAs (siRNAs) and miRNAs, which may contribute to various pathological conditions. Notably, GW182 interacts with endogenous argonaute-2 (Ago2), a key player in the RNA-induced silencing complex, further underscoring GW182′s importance in gene regulation. The presence of autoantibodies against GW182 has been observed in patients with autoimmune diseases such as ataxia, Sjögren′s syndrome, and sensor neuropathy, highlighting GW182′s potential as a biomarker for these conditions. Anti-GW182 antibody (A-8) serves as an invaluable tool for researchers investigating the intricate mechanisms of RNA regulation and the role of GWBs in cellular processes.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
GW182 Antibody (A-8) References:
- GW182 is critical for the stability of GW bodies expressed during the cell cycle and cell proliferation. | Yang, Z., et al. 2004. J Cell Sci. 117: 5567-78. PMID: 15494374
- A crucial role for GW182 and the DCP1:DCP2 decapping complex in miRNA-mediated gene silencing. | Rehwinkel, J., et al. 2005. RNA. 11: 1640-7. PMID: 16177138
- A role for the P-body component GW182 in microRNA function. | Liu, J., et al. 2005. Nat Cell Biol. 7: 1261-6. PMID: 16284623
- Structural insights into the human GW182-PABC interaction in microRNA-mediated deadenylation. | Jinek, M., et al. 2010. Nat Struct Mol Biol. 17: 238-40. PMID: 20098421
- Structural basis of binding of P-body-associated proteins GW182 and ataxin-2 by the Mlle domain of poly(A)-binding protein. | Kozlov, G., et al. 2010. J Biol Chem. 285: 13599-606. PMID: 20181956
- Continuous density gradients to study Argonaute and GW182 complexes associated with the endocytic pathway. | Gibbings, D. 2011. Methods Mol Biol. 725: 63-76. PMID: 21528447
- An efficient system for let-7 microRNA and GW182 protein-mediated deadenylation in vitro. | Fabian, MR., et al. 2011. Methods Mol Biol. 725: 207-17. PMID: 21528456
- An SNP in the trinucleotide repeat region of the TNRC6A gene maps to a major TNGW1 autoepitope in patients with autoantibodies to GW182. | Moser, JJ., et al. 2013. Adv Exp Med Biol. 768: 243-59. PMID: 23224974
- Human GW182 Paralogs Are the Central Organizers for RNA-Mediated Control of Transcription. | Hicks, JA., et al. 2017. Cell Rep. 20: 1543-1552. PMID: 28813667
- KSHV RNA-binding protein ORF57 inhibits P-body formation to promote viral multiplication by interaction with Ago2 and GW182. | Sharma, NR., et al. 2019. Nucleic Acids Res. 47: 9368-9385. PMID: 31400113
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
GW182 Antibody (A-8) | sc-377006 | 200 µg/ml | $322.00 | |||
GW182 Antibody (A-8): m-IgG Fc BP-HRP Bundle | sc-538016 | 200 µg Ab; 10 µg BP | $361.00 | |||
GW182 Antibody (A-8): m-IgGκ BP-HRP Bundle | sc-535535 | 200 µg Ab; 40 µg BP | $361.00 | |||
GW182 Antibody (A-8): m-IgG2a BP-HRP Bundle | sc-548184 | 200 µg Ab; 10 µg BP | $361.00 |