GW182 Antibody (4B6): sc-56314

GW182 Antibody (4B6) is a mouse monoclonal IgG1 kappa light chain antibody that detects GW182 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry. Anti-GW182 antibody (4B6) is available in both conjugated and non-conjugated forms, providing versatility for various experimental needs. GW182 plays a critical role in the formation and function of GW bodies (GWBs), which are essential for the regulation of mRNA stability and degradation. These intracellular structures serve as sites for the storage and processing of mRNAs, making them vital for microRNA (miRNA) function. The integrity of GWBs is crucial, as their disassembly or disruption can significantly impair the silencing activity of small interfering RNAs (siRNAs) and miRNAs, which are key regulators of gene expression. GW182, as a cytoplasmic marker protein for GWBs, not only contributes to the maintenance and stability of these structures but also interacts with endogenous argonaute-2 (Ago2), further emphasizing GW182′s importance in the RNA silencing pathway. Additionally, GW182 has been implicated in various autoimmune conditions, as patients with ataxia, Sjögren′s syndrome, and sensor neuropathy often develop autoantibodies against proteins associated with GWB structures, highlighting GW182′s relevance in both cellular function and disease contexts.

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GW182 Antibody (4B6) References:

1. A phosphorylated cytoplasmic autoantigen, GW182, associates with a unique population of human mRNAs within novel cytoplasmic speckles.  |  Eystathioy, T., et al. 2002. Mol Biol Cell. 13: 1338-51. PMID: 11950943
2. A panel of monoclonal antibodies to cytoplasmic GW bodies and the mRNA binding protein GW182.  |  Eystathioy, T., et al. 2003. Hybrid Hybridomics. 22: 79-86. PMID: 12831532
3. The GW182 protein colocalizes with mRNA degradation associated proteins hDcp1 and hLSm4 in cytoplasmic GW bodies.  |  Eystathioy, T., et al. 2003. RNA. 9: 1171-3. PMID: 13130130
4. Clinical and serological associations of autoantibodies to GW bodies and a novel cytoplasmic autoantigen GW182.  |  Eystathioy, T., et al. 2003. J Mol Med (Berl). 81: 811-8. PMID: 14598044
5. GW182 is critical for the stability of GW bodies expressed during the cell cycle and cell proliferation.  |  Yang, Z., et al. 2004. J Cell Sci. 117: 5567-78. PMID: 15494374
6. A crucial role for GW182 and the DCP1:DCP2 decapping complex in miRNA-mediated gene silencing.  |  Rehwinkel, J., et al. 2005. RNA. 11: 1640-7. PMID: 16177138
7. Disruption of GW bodies impairs mammalian RNA interference.  |  Jakymiw, A., et al. 2005. Nat Cell Biol. 7: 1267-74. PMID: 16284622
8. GW bodies, microRNAs and the cell cycle.  |  Lian, S., et al. 2006. Cell Cycle. 5: 242-5. PMID: 16418578
9. Ago2 and GW182 expression in mouse preimplantation embryos: a link between microRNA biogenesis and GW182 protein synthesis.  |  Shen, XH., et al. 2010. Reprod Fertil Dev. 22: 634-43. PMID: 20353723
10. Defining a new role of GW182 in maintaining miRNA stability.  |  Yao, B., et al. 2012. EMBO Rep. 13: 1102-8. PMID: 23090477
11. Early origin and adaptive evolution of the GW182 protein family, the key component of RNA silencing in animals.  |  Zielezinski, A. and Karlowski, WM. 2015. RNA Biol. 12: 761-70. PMID: 26106978
12. Multivalent Recruitment of Human Argonaute by GW182.  |  Elkayam, E., et al. 2017. Mol Cell. 67: 646-658.e3. PMID: 28781232

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-GW182 Antibody (4B6) has 20 citations in a variety of scientific publications.

Primary antibodies like Anti-GW182 Antibody (4B6) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

GW182 Antibody (4B6) Specifications

Alternate Product Name: GW182

Product Clone Name: 4B6

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids:

Epitope Target: GW182

Epitope Species: human

Product Reactivity/Detection: GW182

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: Drosophila, Xenopus

Applications: WB, IP, IF, IHC(P)

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 20 citations in various scientific publications

Available Conjugates/Forms for GW182 Antibody (4B6): AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790