GSK-3α Antibody (H-12): sc-5264

GSK-3α Antibody (H-12) is a mouse monoclonal IgG1 kappa light chain antibody that detects GSK3A protein of mouse, rat, and human origin by WB, IP, IF, IHCP, and ELISA. GSK-3α Antibody (H-12) is available in both non-conjugated and various conjugated forms, including agarose, HRP, PE, FITC, and multiple Alexa Fluor® conjugates. Glycogen synthase kinase 3 (GSK-3) plays a crucial role in numerous signaling pathways, particularly in glycogen metabolism and cellular adhesion, and has been linked to neurodegenerative diseases such as Alzheimer′s disease. GSK-3α and GSK-3β exhibit distinct subcellular localization, influencing their functional roles within the cell. GSK-3α is primarily localized in the cytoplasm, where GSK-3α phosphorylates various substrates, including the Tau protein, which is essential for microtubule stability in neurons. The phosphorylation of Tau by GSK-3α is particularly significant, as hyperphosphorylated Tau aggregates into paired helical filaments, a hallmark of neurofibrillary tangles found in Alzheimer′s disease. This interaction underscores GSK-3α′s importance in maintaining neuronal health and highlights GSK-3α′s potential as a therapeutic target in neurodegenerative disorders.

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GSK-3α Antibody (H-12) References:

1. Fibroblast GSK-3α Promotes Fibrosis via RAF-MEK-ERK Pathway in the Injured Heart.  |  Umbarkar, P., et al. 2022. Circ Res. 131: 620-636. PMID: 36052698
2. Lomerizine inhibits LPS-mediated neuroinflammation and tau hyperphosphorylation by modulating NLRP3, DYRK1A, and GSK3α/β.  |  Park, JH., et al. 2023. Front Immunol. 14: 1150940. PMID: 37435081
3. Cardiac fibroblast GSK-3α aggravates ischemic cardiac injury by promoting fibrosis, inflammation, and impairing angiogenesis.  |  Umbarkar, P., et al. 2023. Basic Res Cardiol. 118: 35. PMID: 37656238
4. GSK-3α aggravates inflammation, metabolic derangement, and cardiac injury post-ischemia/reperfusion.  |  Ahmad, F., et al. 2023. J Mol Med (Berl). 101: 1379-1396. PMID: 37707557
5. Networking with proline-directed protein kinases implicated in tau phosphorylation.  |  Pelech, SL. 1995. Neurobiol Aging. 16: 247-56; discussion 257-61. PMID: 7566335
6. Glycogen synthase kinase-3 beta phosphorylates tau protein at multiple sites in intact cells.  |  Sperber, BR., et al. 1995. Neurosci Lett. 197: 149-53. PMID: 8552282
7. Regulation of glycogen synthase activation in isolated hepatocytes.  |  Pugazhenthi, S. and Khandelwal, RL. 1995. Mol Cell Biochem. 149-150: 95-101. PMID: 8569754
8. Different localization of tau protein kinase I/glycogen synthase kinase-3 beta from glycogen synthase kinase-3 alpha in cerebellum mitochondria.  |  Hoshi, M., et al. 1995. J Biochem. 118: 683-5. PMID: 8576078
9. Regulation of mitochondrial pyruvate dehydrogenase activity by tau protein kinase I/glycogen synthase kinase 3beta in brain.  |  Hoshi, M., et al. 1996. Proc Natl Acad Sci U S A. 93: 2719-23. PMID: 8610107
10. Binding of GSK3beta to the APC-beta-catenin complex and regulation of complex assembly.  |  Rubinfeld, B., et al. 1996. Science. 272: 1023-6. PMID: 8638126
11. Differential phosphorylation of human tau isoforms containing three repeats by several protein kinases.  |  Singh, TJ., et al. 1996. Arch Biochem Biophys. 328: 43-50. PMID: 8638936
12. Tau is enriched on dynamic microtubules in the distal region of growing axons.  |  Black, MM., et al. 1996. J Neurosci. 16: 3601-19. PMID: 8642405

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-GSK-3α Antibody (H-12) has 14 citations in a variety of scientific publications.

Primary antibodies like Anti-GSK-3α Antibody (H-12) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

GSK-3α Antibody (H-12) Specifications

Alternate Product Name: GSK3

Product Clone Name: H-12

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 464-483

Epitope Target: GSK-3α

Epitope Species: human

Product Reactivity/Detection: GSK-3α

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 14 citations in various scientific publications

Available Conjugates/Forms for GSK-3α Antibody (H-12): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin

The preferred GSK3 alpha/beta antibody is GSK3 alpha/beta Antibody (0011-A): sc-7291.