GRIP-1 Antibody (F-2): sc-365827

GRIP-1 Antibody (F-2) is a mouse monoclonal IgG1 kappa light chain antibody that detects GRIP-1 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). GRIP-1 (F-2) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. GRIP-1 protein, also known as nuclear receptor interacting protein 2 (NCoA-2 or Tif2), plays a crucial role as a transcriptional coactivator, facilitating the action of nuclear receptors for steroids, thyroid hormones, and retinoic acids. By enhancing the transcriptional activity of these receptors, GRIP-1 is vital for regulating gene expression in response to hormonal signals, which is essential for numerous physiological processes, including metabolism, development, and cellular differentiation. Additionally, GRIP-1 interacts with other key proteins in the steroid receptor family, such as SRC-1 and RAC3, which are implicated in various cancers, highlighting its importance in both normal physiology and disease states. Anti-GRIP-1 antibody (F-2) effectively detects this protein across multiple species and applications, making GRIP-1 (F-2) monoclonal antibody an invaluable tool for researchers studying gene regulation and the molecular mechanisms underlying hormone action.

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GRIP-1 Antibody (F-2) References:

1. Characterization of the glutamate receptor-interacting proteins GRIP1 and GRIP2.  |  Dong, H., et al. 1999. J Neurosci. 19: 6930-41. PMID: 10436050
2. Glucocorticoid-dependent phosphorylation of the transcriptional coregulator GRIP1.  |  Dobrovolna, J., et al. 2012. Mol Cell Biol. 32: 730-9. PMID: 22158970
3. Role of transcriptional coregulator GRIP1 in the anti-inflammatory actions of glucocorticoids.  |  Chinenov, Y., et al. 2012. Proc Natl Acad Sci U S A. 109: 11776-81. PMID: 22753499
4. Trip6 promotes dendritic morphogenesis through dephosphorylated GRIP1-dependent myosin VI and F-actin organization.  |  Lv, K., et al. 2015. J Neurosci. 35: 2559-71. PMID: 25673849
5. Purkinje cell-specific Grip1/2 knockout mice show increased repetitive self-grooming and enhanced mGluR5 signaling in cerebellum.  |  Mejias, R., et al. 2019. Neurobiol Dis. 132: 104602. PMID: 31476380
6. GLCCI1 Deficiency Induces Glucocorticoid Resistance via the Competitive Binding of IRF1:GRIP1 and IRF3:GRIP1 in Asthma.  |  Hu, X., et al. 2021. Front Med (Lausanne). 8: 686493. PMID: 34504850
7. Sequence and characterization of a coactivator for the steroid hormone receptor superfamily.  |  Oñate, SA., et al. 1995. Science. 270: 1354-7. PMID: 7481822
8. The nuclear hormone receptor gene superfamily.  |  Ribeiro, RC., et al. 1995. Annu Rev Med. 46: 443-53. PMID: 7598477
9. GRIP1, a novel mouse protein that serves as a transcriptional coactivator in yeast for the hormone binding domains of steroid receptors.  |  Hong, H., et al. 1996. Proc Natl Acad Sci U S A. 93: 4948-52. PMID: 8643509
10. The transcriptional co-activator p/CIP binds CBP and mediates nuclear-receptor function.  |  Torchia, J., et al. 1997. Nature. 387: 677-84. PMID: 9192892
11. RAC3, a steroid/nuclear receptor-associated coactivator that is related to SRC-1 and TIF2.  |  Li, H., et al. 1997. Proc Natl Acad Sci U S A. 94: 8479-84. PMID: 9238002
12. AIB1, a steroid receptor coactivator amplified in breast and ovarian cancer.  |  Anzick, SL., et al. 1997. Science. 277: 965-8. PMID: 9252329

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-GRIP-1 Antibody (F-2) has 2 citations in a variety of scientific publications.

Primary antibodies like Anti-GRIP-1 Antibody (F-2) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

GRIP-1 Antibody (F-2) Specifications

Alternate Product Name: GRIP-1

Product Clone Name: F-2

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 787-1129

Epitope Target: GRIP-1

Epitope Species: mouse

Product Reactivity/Detection: GRIP-1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 2 citations in various scientific publications

Available Conjugates/Forms for GRIP-1 Antibody (F-2): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin, X: TransCruz Reagent