Anti-GR/NR3C1/Glucocorticoid Receptor Antibody (G-5) recommended for detection of GR α and GR β of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

Anti-GR/NR3C1/Glucocorticoid Receptor Antibody (G-5): sc-393232

Anti-GR/NR3C1/Glucocorticoid Receptor Antibody (G-5) is rated 4.0 out of 5 by 27.
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Datasheets

    • Anti-GR/NR3C1/Glucocorticoid Receptor Antibody (G-5) is a mouse monoclonal IgG2b κ GR/NR3C1/Glucocorticoid Receptor antibody, cited in 49 publications, provided at 200 µg/ml
    • raised against amino acids 121-420 of GR of human origin
    • Anti-GR/NR3C1/Glucocorticoid Receptor Antibody (G-5) is recommended for detection of GR α and GR β of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
    • Anti-GR/NR3C1/Glucocorticoid Receptor Antibody (G-5) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • TransCruz reagent for Gel Supershift and ChIP applications (sc-393232 X, 200 µg/0.1 ml)
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of GR/NR3C1/Glucocorticoid Receptor (G-5): sc-393232.
    • m-IgG Fc BP-HRP and m-IgG2b BP-HRP are the preferred secondary detection reagents for GR/NR3C1/Glucocorticoid Receptor Antibody (G-5) for WB and IHC(P) applications. These reagents are now offered in bundles with GR/NR3C1/Glucocorticoid Receptor Antibody (G-5) (see ordering information below).
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Setting: Western Blot 20 mcg protein of tissue lysate (mouse tissue); antibody diluted 1:1000 in 5%BSA. Problem: I have a good band at 95 kDa , but also a clear (unexpected) band around 50kDa. What could this be?

Asked by: Arnot
Thank you for your question. It would be helpful if you could call us, allowing for a more interactive discussion of this and other related questions.
Answered by: Technical Support
Date published: 2021-07-22

Does GR antibody (G-5): sc-393232 work in ChIP assay for rat muscle?

Asked by: jymrobin
Thank you for your question. This antibody has been published previously for ChIP applications (PMID: 30994266 and PMID: 30698747). This antibody is reactive with mouse, rat and human. We do not have any data specifically in rat muscle.
Answered by: Technical Support
Date published: 2019-07-18

How well does the antibody work with rat brain tissue for western blotting, and what would be the optimum concentration?

Asked by: nico315
Thank you for your question. We have not yet tested this antibody in rat brain tissue. We do have data in mouse brain tissue extract. The primary antibody was used at the starting dilution of 1:200, and loading 50ug tissue extract.
Answered by: Technical Support
Date published: 2019-07-13

Is the GR ANTIBODY (G-5) X sc-393232X the same as the GR ANTIBODY (G-5) sc-393232 but just at a higher concentration?

Asked by: MegCon
Thank you for your question. Yes, sc-393232 X is provided at a 10x higher concentration. It is provided as 200µg in 0.1ml while the standard product is provided as 200µg in 1ml.
Answered by: Tech Service
Date published: 2019-01-14

Hi, please let me know what concentration of the  GR Antibody (G-5): sc-393232 would be optimal for  Flow Cytometry.  Thank you.

Asked by: nacharya18
Thank you for your question. We have not yet tested this mouse monoclonal, GR Antibody (G-5): sc-393232, for Flow Cytometry so this application is not recommended at this time. We do offer this antibody directly conjugated to a variety of fluorochromes, including: phycoerythrin (sc-393232 PE), fluorescein (sc-393232 FITC), Alexa Fluor® 488 (sc-393232 AF488) or Alexa Fluor® 647 (sc-393232 AF647), 200 µg/ml, for IF, IHC(P) and FCM. If you purchase one of the direct conjugates, we can extend our product warranty to cover the application of Flow Cytometry. A link to our recommended protocol for FCM is below. We recommend using approximately 1 µg of fluorochrome-conjugated antibody to 100 µl of the prepared cell suspension (equal to 1 million cells) to each tube. https://www.scbt.com/scbt/resources/protocols/flow-cytometry
Answered by: Technical Support
Date published: 2017-03-03

Hi, I am a graduate student working in a Xenopus Laevis laboratory, I would like to test this antibody in our system, is it possible to get an aliquot of it to test it? 

Asked by: Northwestern University
Thank you for your question. We do not know if this mouse monoclonal, GR Antibody (G-5): sc-393232, will work for Xenopus Laevis. For a limited time, we are currently offering free 10 µg samples to test of the antibody. Please contact our Technical Service Department to request a sample. Please call us toll-free at (800) 457-3801 option 2, e-mail [email protected], or use our live chat!
Answered by: Technical Support
Date published: 2017-02-24

I need to use an antibody to detect the protein in samples of avian origin. Do you know if this mouse monoclonal antibody will work?

Asked by: jerojero
Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity.
Answered by: Technical Service 11
Date published: 2017-01-11

What is the recommended secondary reagent to be used with this mouse monoclonal primary?

Asked by: jerojero
We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Service 11
Date published: 2017-01-11
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Rated 4 out of 5 by from Good for WB of mouse hippocampus We ordered the sample and proved it for WB of mice hippocampal tissue. Gave a nice specific band for the GR as assessed in WT and Emx1-Nr3c1 KO mice.
Date published: 2021-08-03
Rated 4 out of 5 by from Glucocorticoid receptor antibody Good staining of mice hypothalamus. Sample: mouse cortex fixed with 4% PFA for 16hr at 4℃, followed by sucrose infiltration and OCT embedding. Staining: floatation staining for 48hr at 4℃ (1:300 or 1:500, 300 was better), followed by 2nd antibody staining. FYI, I have not tried shorter staining such as 1hr at RT or overnight at 4℃.
Date published: 2021-07-28
Rated 3 out of 5 by from Glucocorticoid receptor antibody This antibody works better in nuclear then in cytoplasmic fraction of liver (dilution 1:250).
Date published: 2021-02-17
Rated 3 out of 5 by from Glucocorticoid receptor antibody This antibody works better in nuclear then in cytoplasmic fraction of liver (dilution 1:250).
Date published: 2021-02-17
Rated 5 out of 5 by from Works excelent in westernblot GR was detected in mesenchymal stem cells and used at a dilution of 1;250 1 H at room temp
Date published: 2020-09-18
Rated 5 out of 5 by from PERFECT CHOICE This antibody works well. Used 1:500 in tbs 0,1%tween to detect GR in different cell line extract (45gs of total proteins; MCF-7 breast cancer cells and KP2 and SUIT2 pancreatic cancer cells), detect only one specific band at 95 kDa. I’ll buy this antibody.
Date published: 2019-09-27
Rated 5 out of 5 by from Works well! It works well for ChIP on both mouse hepatocytes and mouse livers.
Date published: 2019-04-26
Rated 5 out of 5 by from Works Beautifully I've got a sample of this AB and used to perform IF in mouse brain tissue. I've obtained a nice staining as using a 1:1000 dilution. I've ordered the product already
Date published: 2018-04-23
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