Glutathione Peroxidase 1/2/GPX1/2 Antibody (D-12): sc-133152

GPx-1/2 Antibody (D-12) is a mouse monoclonal IgG1 antibody that detects glutathione peroxidase (GPx) in mouse, rat, and human samples through western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). GPx enzymes, particularly GPx-1 and GPx-2, are crucial for cellular defense against oxidative stress, as these enzymes catalyze the reduction of lipid hydroperoxides to alcohols and convert free hydrogen peroxide into water, thereby preventing lipid peroxidation of cell membranes. The presence of selenocysteine at the active site of GPx enzymes, which is encoded by the stop codon TGA, is vital for enzymatic activity and antioxidant function. GPx-1 is predominantly expressed in vascular and neural tissues, playing a significant role in protecting these cells from oxidative damage, while GPx-2 is the major isoform in the lungs and GPx-2 expression is tightly regulated by the transcription factor Nrf2, highlighting GPx-2′s importance in lung health and response to oxidative stress. Anti-GPx-1/2 antibody (D-12) effectively recognizes these proteins, making this antibody an invaluable tool for researchers studying oxidative stress and its implications in various diseases, including cardiovascular conditions and neurodegenerative disorders.

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Glutathione Peroxidase 1/2/GPX1/2 Antibody (D-12) References:

1. The effects of GPX-1 knockout on membrane transport and intracellular homeostasis in the lens.  |  Wang, H., et al. 2009. J Membr Biol. 227: 25-37. PMID: 19067024
2. Genetic association of Glutathione peroxidase-1 (GPx-1) and NAD(P)H:Quinone Oxidoreductase 1(NQO1) variants and their association of CAD in patients with type-2 diabetes.  |  Ramprasath, T., et al. 2012. Mol Cell Biochem. 361: 143-50. PMID: 21989715
3. The effects of selenium and the GPx-1 selenoprotein on the phosphorylation of H2AX.  |  Jerome-Morais, A., et al. 2013. Biochim Biophys Acta. 1830: 3399-406. PMID: 23518201
4. Translational regulation of GPx-1 and GPx-4 by the mTOR pathway.  |  Reinke, EN., et al. 2014. PLoS One. 9: e93472. PMID: 24691473
5. Association of glutathione peroxidase-1 (GPx-1) rs1050450 Pro198Leu and Pro197Leu polymorphisms with cardiovascular risk: a meta-analysis of observational studies.  |  Zhang, JX., et al. 2014. J Geriatr Cardiol. 11: 141-50. PMID: 25009565
6. The effect of GPx-1 rs1050450 and MnSOD rs4880 polymorphisms on PE susceptibility: a case- control study.  |  Teimoori, B., et al. 2019. Mol Biol Rep. 46: 6099-6104. PMID: 31512047
7. Targeting c-Src Reverses Accelerated GPX-1 mRNA Decay in Chronic Obstructive Pulmonary Disease Airway Epithelial Cells.  |  Dabo, AJ., et al. 2020. Am J Respir Cell Mol Biol. 62: 598-607. PMID: 31801023
8. Association between GPx-1 polymorphisms and personality traits in healthy Chinese-Han subjects.  |  Shao, X., et al. 2020. Brain Behav. 10: e01897. PMID: 33070477
9. Ginsenoside Re attenuates memory impairments in aged Klotho deficient mice via interactive modulations of angiotensin II AT1 receptor, Nrf2 and GPx-1 gene.  |  Nguyen, BT., et al. 2022. Free Radic Biol Med. 189: 2-19. PMID: 35840016
10. Mountain-cultivated ginseng protects against cognitive impairments in aged GPx-1 knockout mice via activation of Nrf2/ChAT/ERK signaling pathway.  |  Nguyen, BT., et al. 2023. J Ginseng Res. 47: 561-571. PMID: 37397414

The preferred Glutathione Peroxidase 1/2/GPX1/2 antibody is Glutathione Peroxidase 1/2/GPX1/2 Antibody (B-6): sc-133160.