GPR14 Antibody (D-4): sc-514460

GPR14 Antibody (D-4) is a mouse monoclonal IgG1 kappa light chain antibody that detects GPR14 of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-GPR14 antibody (D-4) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. GPR14, also known as G-protein-coupled receptor or sensory epithelium neuropeptide-like receptor, was initially identified as an ′orphan′ receptor, meaning GPR14 binds to an unidentified natural ligand. Subsequent research revealed that urotensin II, a cyclic neuropeptide, binds to GPR14 with high affinity, leading to an increase in intracellular calcium levels in cells expressing GPR14. GPR14 localization in heart tissues is particularly significant, as GPR14 suggests a role in vascular smooth muscle contraction in response to urotensin II binding, which is crucial for regulating blood pressure and cardiovascular function. Additionally, GPR14 expression occurs in the pancreas and, to a lesser extent, in brain tissues, indicating GPR14′s potential involvement in various physiological processes across different organ systems.

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GPR14 Antibody (D-4) References:

1. Cloning, sequence analysis and tissue distribution of the mouse and rat urotensin II precursors.  |  Coulouarn, Y., et al. 1999. FEBS Lett. 457: 28-32. PMID: 10486557
2. Human urotensin-II is a potent vasoconstrictor and agonist for the orphan receptor GPR14.  |  Ames, RS., et al. 1999. Nature. 401: 282-6. PMID: 10499587
3. Urotensin II is the endogenous ligand of a G-protein-coupled orphan receptor, SENR (GPR14).  |  Mori, M., et al. 1999. Biochem Biophys Res Commun. 265: 123-9. PMID: 10548501
4. Identification of the natural ligand of an orphan G-protein-coupled receptor involved in the regulation of vasoconstriction.  |  Nothacker, HP., et al. 1999. Nat Cell Biol. 1: 383-5. PMID: 10559967
5. Identification of urotensin II as the endogenous ligand for the orphan G-protein-coupled receptor GPR14.  |  Liu, Q., et al. 1999. Biochem Biophys Res Commun. 266: 174-8. PMID: 10581185
6. Co-expression of urotensin II and its receptor (GPR14) in human cardiovascular and renal tissues.  |  Matsushita, M., et al. 2001. J Hypertens. 19: 2185-90. PMID: 11725162
7. Urotensin II mediates ERK1/2 phosphorylation and proliferation in GPR14-transfected cell lines.  |  Ziltener, P., et al. 2002. J Recept Signal Transduct Res. 22: 155-68. PMID: 12503613
8. Cellular distribution of GPR14 and the positive inotropic role of urotensin II in the myocardium in adult rat.  |  Gong, H., et al. 2004. J Appl Physiol (1985). 97: 2228-35. PMID: 15273242
9. Ligands and signaling of the G-protein-coupled receptor GPR14, expressed in human kidney cells.  |  Lehner, U., et al. 2007. Cell Physiol Biochem. 20: 181-92. PMID: 17595527
10. UII/GPR14 is involved in NF-κB-mediated colonic inflammation in vivo and in vitro.  |  Yang, Y., et al. 2016. Oncol Rep. 36: 2800-2806. PMID: 27600191
11. Functional genomics: the search for novel neurotransmitters and neuropeptides.  |  Civelli, O. 1998. FEBS Lett. 430: 55-8. PMID: 9678594
12. Cloning of the cDNA encoding the urotensin II precursor in frog and human reveals intense expression of the urotensin II gene in motoneurons of the spinal cord.  |  Coulouarn, Y., et al. 1998. Proc Natl Acad Sci U S A. 95: 15803-8. PMID: 9861051

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-GPR14 Antibody (D-4) has 7 citations in a variety of scientific publications.

Primary antibodies like Anti-GPR14 Antibody (D-4) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

GPR14 Antibody (D-4) Specifications

Alternate Product Name: GPR14

Product Clone Name: D-4

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 10-90

Epitope Target: GPR14

Epitope Species: human

Product Reactivity/Detection: GPR14

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 7 citations in various scientific publications

Available Conjugates/Forms for GPR14 Antibody (D-4): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin