GPA2 Antibody (G-3): sc-390194

GPA2 Antibody (G-3) is a mouse monoclonal IgG1 kappa light chain antibody that detects GPA2 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). GPA2 Antibody (G-3) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. The glycoprotein hormone alpha-2 subunit (GPA2) is a crucial component of the dimeric glycoprotein hormone family, specifically known for forming a heterodimer with glycoprotein hormone beta-5 subunit (GPB5), also referred to as thyrostimulin hormone. This interaction activates the thyroid-stimulating hormone receptor (TSHR), leading to increased cyclic adenosine monophosphate (cAMP) production, which is essential for stimulating the thymus and regulating various physiological processes. GPA2 is characterized by N-linked glycosylation and contains ten cysteine residues likely involved in forming a cysteine knot, a structural feature contributing to stability and function. GPA2′s ubiquitous expression, along with co-localization with GPB5 in key tissues such as the eye, testis, and anterior lobe of the pituitary gland, underscores importance in endocrine signaling and developmental processes.

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LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

GPA2 Antibody (G-3) References:

1. Evolution of glycoprotein hormone subunit genes in bilateral metazoa: identification of two novel human glycoprotein hormone subunit family genes, GPA2 and GPB5.  |  Hsu, SY., et al. 2002. Mol Endocrinol. 16: 1538-51. PMID: 12089349
2. The G protein-coupled receptor Gpr1 and the Galpha protein Gpa2 act through the cAMP-protein kinase A pathway to induce morphogenesis in Candida albicans.  |  Maidan, MM., et al. 2005. Mol Biol Cell. 16: 1971-86. PMID: 15673611
3. Direct activation of fission yeast adenylate cyclase by the Gpa2 Galpha of the glucose signaling pathway.  |  Ivey, FD. and Hoffman, CS. 2005. Proc Natl Acad Sci U S A. 102: 6108-13. PMID: 15831585
4. Heterodimeric fly glycoprotein hormone-alpha2 (GPA2) and glycoprotein hormone-beta5 (GPB5) activate fly leucine-rich repeat-containing G protein-coupled receptor-1 (DLGR1) and stimulation of human thyrotropin receptors by chimeric fly GPA2 and human GPB5.  |  Sudo, S., et al. 2005. Endocrinology. 146: 3596-604. PMID: 15890769
5. Galpha subunit Gpa2 recruits kelch repeat subunits that inhibit receptor-G protein coupling during cAMP-induced dimorphic transitions in Saccharomyces cerevisiae.  |  Harashima, T. and Heitman, J. 2005. Mol Biol Cell. 16: 4557-71. PMID: 16030250
6. Differential expression of the thyrostimulin subunits, glycoprotein alpha2 and beta5 in the rat pituitary.  |  Nagasaki, H., et al. 2006. J Mol Endocrinol. 37: 39-50. PMID: 16901922

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-GPA2 Antibody (G-3) has 5 citations in a variety of scientific publications.

Primary antibodies like Anti-GPA2 Antibody (G-3) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

GPA2 Antibody (G-3) Specifications

Alternate Product Name: GPA2

Product Clone Name: G-3

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 1-129

Epitope Target: GPA2

Epitope Species: human

Product Reactivity/Detection: GPA2

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 5 citations in various scientific publications

Available Conjugates/Forms for GPA2 Antibody (G-3): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin