gp91phox/CYBB/NOX2 Antibody (54.1): sc-130543

gp91-phox Antibody (54.1) is a mouse monoclonal IgG1 kappa light chain antibody that detects gp91-phox in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry. anti-gp91-phox antibody (54.1) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. gp91-phox, also known as cytochrome b-245 beta chain, plays a crucial role in the function of the NADPH oxidase complex, which is essential for generating reactive oxygen species in phagocytes and other cell types. gp91-phox is primarily located in the membrane of phagocytic cells, where gp91-phox forms part of the flavocytochrome cytochrome b558 alongside the p22-phox subunit. gp91-phox localization allows for rapid generation of superoxide in response to various stimuli, which is critical for immune response and pathogen clearance. gp91-phox serves as a surface oxygen sensor, initiating cellular signaling pathways in response to hypoxia, thereby influencing various physiological processes. Distinct expression patterns of gp91-phox in immune cells, compared to its homolog Mox1, highlight gp91-phox specialized role in the immune system, making gp91-phox monoclonal antibody (54.1) an invaluable tool for researchers studying oxidative stress, immune responses, and related signaling pathways.

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gp91phox/CYBB/NOX2 Antibody (54.1) References:

1. O2 sensing is preserved in mice lacking the gp91 phox subunit of NADPH oxidase.  |  Archer, SL., et al. 1999. Proc Natl Acad Sci U S A. 96: 7944-9. PMID: 10393927
2. Cell transformation by the superoxide-generating oxidase Mox1.  |  Suh, YA., et al. 1999. Nature. 401: 79-82. PMID: 10485709
3. Superoxide generation in transformed B-lymphocytes from patients with severe, malignant osteopetrosis.  |  Yang, S., et al. 1999. Mol Cell Biochem. 199: 15-24. PMID: 10544947
4. Identification of a functional leukocyte-type NADPH oxidase in human endothelial cells:a potential atherogenic source of reactive oxygen species.  |  Meyer, JW., et al. 1999. Endothelium. 7: 11-22. PMID: 10599557
5. Evidence for the involvement of distinct signal transduction pathways in the regulation of constitutive and interferon gamma-dependent gene expression of NADPH oxidase components (gp91-phox, p47-phox, and p22-phox) and high-affinity receptor for IgG (Fc gamma R-I) in human polymorphonuclear leukocytes.  |  Amezaga, MA., et al. 1992. Blood. 79: 735-44. PMID: 1531037
6. Prodigiosin inhibits gp91(phox) and iNOS expression to protect mice against the oxidative/nitrosative brain injury induced by hypoxia-ischemia.  |  Chang, CC., et al. 2011. Toxicol Appl Pharmacol. 257: 137-47. PMID: 21925195
7. Monocytic AML cells inactivate antileukemic lymphocytes: role of NADPH oxidase/gp91(phox) expression and the PARP-1/PAR pathway of apoptosis.  |  Aurelius, J., et al. 2012. Blood. 119: 5832-7. PMID: 22550344
8. AAV-based RNAi silencing of NADPH oxidase gp91(phox) attenuates cold-induced cardiovascular dysfunction.  |  Wang, X., et al. 2012. Hum Gene Ther. 23: 1016-26. PMID: 22888847
9. Superoxide anion production and expression of gp91(phox) and p47(phox) are increased in glomeruli and proximal tubules of cisplatin-treated rats.  |  Trujillo, J., et al. 2015. J Biochem Mol Toxicol. 29: 149-56. PMID: 25388649
10. Resveratrol strongly enhances the retinoic acid-induced superoxide generating activity via up-regulation of gp91-phox gene expression in U937 cells.  |  Kikuchi, H., et al. 2018. Biochem Biophys Res Commun. 495: 1195-1200. PMID: 29183727
11. The arachidonate-activable, NADPH oxidase-associated H+ channel. Evidence that gp91-phox functions as an essential part of the channel.  |  Henderson, LM., et al. 1995. J Biol Chem. 270: 5909-16. PMID: 7890722
12. p22phox is a critical component of the superoxide-generating NADH/NADPH oxidase system and regulates angiotensin II-induced hypertrophy in vascular smooth muscle cells.  |  Ushio-Fukai, M., et al. 1996. J Biol Chem. 271: 23317-21. PMID: 8798532

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-gp91phox/CYBB/NOX2 Antibody (54.1) has 146 citations in a variety of scientific publications.

Primary antibodies like Anti-gp91phox/CYBB/NOX2 Antibody (54.1) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

gp91phox/CYBB/NOX2 Antibody (54.1) Specifications

Alternate Product Name: CYBB

Product Clone Name: 54.1

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 383-390

Epitope Target: gp91-phox

Epitope Species: human

Product Reactivity/Detection: gp91-phox

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P)

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 146 citations in various scientific publications

Available Conjugates/Forms for gp91phox/CYBB/NOX2 Antibody (54.1): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin