GP73 Antibody (F-2): sc-365817

GP73 Antibody (F-2) is a mouse monoclonal IgG1 kappa light chain antibody that detects GP73 of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). GP73 (F-2) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. GP73, also known as Golgi phosphoprotein 2, GOLPH2, or Golgi membrane protein, is a type II transmembrane protein predominantly located in the Golgi apparatus, where GP73 plays a crucial role in post-translational modification of proteins synthesized in the rough endoplasmic reticulum. This modification is vital for proper protein folding, stability, and function, which are essential for maintaining cellular homeostasis and facilitating intracellular transport. Notably, GP73 levels are significantly elevated in individuals with acute and chronic liver diseases, making GP73 a potential biomarker for liver dysfunction. The human GP73 gene is mapped to chromosome 9q21.33, highlighting GP73′s genetic significance in health and disease.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

GP73 Antibody (F-2) References:

1. GP73, a novel Golgi-localized protein upregulated by viral infection.  |  Kladney, RD., et al. 2000. Gene. 249: 53-65. PMID: 10831838
2. Expression of GP73, a resident Golgi membrane protein, in viral and nonviral liver disease.  |  Kladney, RD., et al. 2002. Hepatology. 35: 1431-40. PMID: 12029628
3. Upregulation of the Golgi protein GP73 by adenovirus infection requires the E1A CtBP interaction domain.  |  Kladney, RD., et al. 2002. Virology. 301: 236-46. PMID: 12359426
4. Disease- and cell-specific expression of GP73 in human liver disease.  |  Iftikhar, R., et al. 2004. Am J Gastroenterol. 99: 1087-95. PMID: 15180730
5. GP73 and liver disease: a (Golgi) complex enigma.  |  Maitra, A. and Thuluvath, PJ. 2004. Am J Gastroenterol. 99: 1096-8. PMID: 15180731
6. Use of targeted glycoproteomics to identify serum glycoproteins that correlate with liver cancer in woodchucks and humans.  |  Block, TM., et al. 2005. Proc Natl Acad Sci U S A. 102: 779-84. PMID: 15642945
7. Fine mapping of radiation susceptibility and gene expression analysis of LEC congenic rat lines.  |  Tsuji, AB., et al. 2005. Genomics. 86: 271-9. PMID: 15978772
8. GP73, a resident Golgi glycoprotein, is a novel serum marker for hepatocellular carcinoma.  |  Marrero, JA., et al. 2005. J Hepatol. 43: 1007-12. PMID: 16137783
9. GP73 is a glucogenic hormone contributing to SARS-CoV-2-induced hyperglycemia.  |  Wan, L., et al. 2022. Nat Metab. 4: 29-43. PMID: 34992299
10. Deficiency of ASGR1 promotes liver injury by increasing GP73-mediated hepatic endoplasmic reticulum stress.  |  Zhang, Z., et al. 2024. Nat Commun. 15: 1908. PMID: 38459023
11. PTEN deficiency potentiates HBV-associated liver cancer development through augmented GP73/GOLM1.  |  Huang, F., et al. 2024. J Transl Med. 22: 254. PMID: 38459588
12. Integrating Single-Cell and Spatial Transcriptomics to Uncover and Elucidate GP73-Mediated Pro-Angiogenic Regulatory Networks in Hepatocellular Carcinoma.  |  Ye, J., et al. 2024. Research (Wash D C). 7: 0387. PMID: 38939041

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-GP73 Antibody (F-2) has 4 citations in a variety of scientific publications.

Primary antibodies like Anti-GP73 Antibody (F-2) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

GP73 Antibody (F-2) Specifications

Alternate Product Name: GOLM1

Product Clone Name: F-2

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 1-114

Epitope Target: GP73

Epitope Species: human

Product Reactivity/Detection: GP73

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 4 citations in various scientific publications

Available Conjugates/Forms for GP73 Antibody (F-2): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin