CD130/gp130 Antibody (AN-H2): sc-9994
- CD130/gp130 Antibody (AN-H2) is a mouse monoclonal IgG1 κ, cited in 10 publications, provided at 200 µg/ml
- raised against gp130 transfected Sf21 cells of human origin
- CD130/gp130 Antibody (AN-H2) is recommended for detection of gp130 of human origin by WB, IP, IF, IHC(P), FCM and ELISA
- Anti-CD130/gp130 Antibody (AN-H2) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for CD130/gp130 Antibody (AN-H2) for WB and IHC(P) applications. These reagents are now offered in bundles with CD130/gp130 Antibody (AN-H2) (see ordering information below).
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gp130 (AN-H2) is a mouse monoclonal IgG1 kappa light chain antibody that detects gp130 of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), flow cytometry (FCM) and enzyme-linked immunosorbent assay (ELISA). gp130 monoclonal antibody (AN-H2) is available as the non-conjugated form, as well as multiple conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC) and multiple Alexa Fluor® conjugates. IL-6 activates intracellular signaling through binding a receptor consisting of a ligand-binding protein (IL-6R) and a second protein. IL-6 first binds to IL-6R which subsequently associates with a gp130 dimer. The active signaling complex consists of at minimum IL-6, IL-6R and a dimer of two gp130 proteins that are linked by a disulfide bond. A soluble form of IL-6R is generated by proteolytic cleavage of the membrane-bound precursor and can function as an agonistic molecule that can actively participate in cell-to-cell signaling. The second subunit of the IL-6 complex, gp130, also functions as a component of several additional receptor complexes including leukemia inhibitory factor (LIF), oncostatin M (OSM), ciliary neurotrophic factor (CNTF) and IL-11. LIF binds to the LIF receptor with low affinity and to a complex of the LIF receptor and gp130 with high affinity while OSM appears to bind to gp130 with low affinity and to a complex of gp130 and the LIF receptor with high affinity.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
CD130/gp130 Antibody (AN-H2) References:
- gp130 Controls Cardiomyocyte Proliferation and Heart Regeneration. | Li, Y., et al. 2020. Circulation. 142: 967-982. PMID: 32600062
- The Role of gp130 Cytokines in Tuberculosis. | Ritter, K., et al. 2020. Cells. 9: PMID: 33334075
- A myeloid-stromal niche and gp130 rescue in NOD2-driven Crohn's disease. | Nayar, S., et al. 2021. Nature. 593: 275-281. PMID: 33789339
- β-Klotho promotes glycolysis and glucose-stimulated insulin secretion via GP130. | Geng, L., et al. 2022. Nat Metab. 4: 608-626. PMID: 35551509
- Inhibition of a signaling modality within the gp130 receptor enhances tissue regeneration and mitigates osteoarthritis. | Shkhyan, R., et al. 2023. Sci Transl Med. 15: eabq2395. PMID: 36947594
- Gp130 Promotes Inflammation via the STAT3/JAK2 Pathway in Allergic Conjunctivitis. | Bao, J., et al. 2023. Invest Ophthalmol Vis Sci. 64: 5. PMID: 37022703
- CD9 exacerbates pathological cardiac hypertrophy through regulating GP130/STAT3 signaling pathway. | Li, Y., et al. 2023. iScience. 26: 108070. PMID: 37860696
- Structures of the interleukin 11 signalling complex reveal gp130 dynamics and the inhibitory mechanism of a cytokine variant. | Metcalfe, RD., et al. 2023. Nat Commun. 14: 7543. PMID: 37985757
- The Human GP130 Cytokine Receptor and Its Expression-an Atlas and Functional Taxonomy of Genetic Variants. | Chen, YH., et al. 2023. J Clin Immunol. 44: 30. PMID: 38133879
- IL-6/gp130 signaling in CD4+ T cells drives the pathogenesis of pulmonary hypertension. | Ishibashi, T., et al. 2024. Proc Natl Acad Sci U S A. 121: e2315123121. PMID: 38602915
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CD130/gp130 Antibody (AN-H2) | sc-9994 | 200 µg/ml | $322.00 | |||
CD130/gp130 Antibody (AN-H2): m-IgG Fc BP-HRP Bundle | sc-528249 | 200 µg Ab; 10 µg BP | $361.00 | |||
CD130/gp130 Antibody (AN-H2): m-IgGκ BP-HRP Bundle | sc-520578 | 200 µg Ab, 40 µg BP | $361.00 | |||
CD130/gp130 Antibody (AN-H2): m-IgG1 BP-HRP Bundle | sc-542839 | 200 µg Ab; 20 µg BP | $361.00 | |||
CD130/gp130 Antibody (AN-H2) AC | sc-9994 AC | 500 µg/ml, 25% agarose | $424.00 | |||
CD130/gp130 Antibody (AN-H2) HRP | sc-9994 HRP | 200 µg/ml | $322.00 | |||
CD130/gp130 Antibody (AN-H2) FITC | sc-9994 FITC | 200 µg/ml | $336.00 | |||
CD130/gp130 Antibody (AN-H2) PE | sc-9994 PE | 200 µg/ml | $349.00 | |||
CD130/gp130 Antibody (AN-H2) Alexa Fluor® 488 | sc-9994 AF488 | 200 µg/ml | $364.00 | |||
CD130/gp130 Antibody (AN-H2) Alexa Fluor® 546 | sc-9994 AF546 | 200 µg/ml | $364.00 | |||
CD130/gp130 Antibody (AN-H2) Alexa Fluor® 594 | sc-9994 AF594 | 200 µg/ml | $364.00 | |||
CD130/gp130 Antibody (AN-H2) Alexa Fluor® 647 | sc-9994 AF647 | 200 µg/ml | $364.00 | |||
CD130/gp130 Antibody (AN-H2) Alexa Fluor® 680 | sc-9994 AF680 | 200 µg/ml | $364.00 | |||
CD130/gp130 Antibody (AN-H2) Alexa Fluor® 790 | sc-9994 AF790 | 200 µg/ml | $364.00 |