golgin 45 Antibody (E-3): sc-515193
- golgin 45 Antibody (E-3) is a mouse monoclonal IgG1 κ golgin 45 antibody, cited in 1 publications, provided at 200 µg/ml
- raised against amino acids 1-300 mapping at the N-terminus of golgin 45 of human origin
- golgin 45 Antibody (E-3) is recommended for detection of golgin 45 isoforms 1 and 2 of human origin by WB, IP, IF and ELISA
- Anti-golgin 45 Antibody (E-3) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP is the preferred secondary detection reagent for golgin 45 Antibody (E-3) for WB applications. This reagent is now offered in a bundle with golgin 45 Antibody (E-3) (see ordering information below).
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golgin 45 Antibody (E-3) is a mouse monoclonal IgG1 kappa light chain antibody that detects golgin 45 of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). anti-golgin 45 antibody (E-3) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. golgin 45, also known as JEM-1 or Basic Leucine Zipper Nuclear Factor 1, is a 400 amino acid protein primarily localized to the lumen of the Golgi apparatus, where golgin 45 plays a crucial role in maintaining normal Golgi structure and facilitating protein transport from the endoplasmic reticulum to the cell surface. This transport process is essential for cellular function and is primarily regulated through golgin 45 interaction with GRASP55, forming a Rab 2 effector complex vital for Golgi organization and function. Notably, golgin 45 is expressed in two isoforms, with one localizing to the nucleus and functioning as a transcription factor, highlighting multiple roles in cellular processes.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
golgin 45 Antibody (E-3) References:
- JEM-1, a novel nuclear co-factor: localisation and functional interaction with AP-1. | Tong, JH., et al. 1999. Leukemia. 13: 1982-92. PMID: 10602419
- Genomic organization of the JEM-1 (BLZF1) gene on human chromosome 1q24: molecular cloning and analysis of its promoter region. | Tong, JH., et al. 2000. Genomics. 69: 380-90. PMID: 11056056
- A GRASP55-rab2 effector complex linking Golgi structure to membrane traffic. | Short, B., et al. 2001. J Cell Biol. 155: 877-83. PMID: 11739401
- JEM-1, a novel gene encoding a leucine-zipper nuclear factor upregulated during retinoid-induced maturation of NB4 promyelocytic leukaemia. | Duprez, E., et al. 1997. Oncogene. 14: 1563-70. PMID: 9129147
- Expression patterns of the JEM-1 gene in normal and tumor cells: ubiquity contrasting with a faint, but retinoid-induced, mRNA expression in promyelocytic NB4 cells. | Tong, JH., et al. 1998. Leukemia. 12: 1733-40. PMID: 9823948
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
golgin 45 Antibody (E-3) | sc-515193 | 200 µg/ml | $316.00 | |||
golgin 45 Antibody (E-3): m-IgG Fc BP-HRP Bundle | sc-526421 | 200 µg Ab; 10 µg BP | $354.00 | |||
golgin 45 Antibody (E-3) AC | sc-515193 AC | 500 µg/ml, 25% agarose | $416.00 | |||
golgin 45 Antibody (E-3) HRP | sc-515193 HRP | 200 µg/ml | $316.00 | |||
golgin 45 Antibody (E-3) FITC | sc-515193 FITC | 200 µg/ml | $330.00 | |||
golgin 45 Antibody (E-3) PE | sc-515193 PE | 200 µg/ml | $343.00 | |||
golgin 45 Antibody (E-3) Alexa Fluor® 488 | sc-515193 AF488 | 200 µg/ml | $357.00 | |||
golgin 45 Antibody (E-3) Alexa Fluor® 546 | sc-515193 AF546 | 200 µg/ml | $357.00 | |||
golgin 45 Antibody (E-3) Alexa Fluor® 594 | sc-515193 AF594 | 200 µg/ml | $357.00 | |||
golgin 45 Antibody (E-3) Alexa Fluor® 647 | sc-515193 AF647 | 200 µg/ml | $357.00 | |||
golgin 45 Antibody (E-3) Alexa Fluor® 680 | sc-515193 AF680 | 200 µg/ml | $357.00 | |||
golgin 45 Antibody (E-3) Alexa Fluor® 790 | sc-515193 AF790 | 200 µg/ml | $357.00 |