Anti-GnRH I Antibody (A-4) is a mouse monoclonal IgG2b (kappa light chain) GnRH I antibody provided at 200 µg/ml
raised against amino acids 1-92 representing full length Progonadoliberin I of human origin
GnRH I Antibody (A-4) is recommended for detection of Progonadoliberin I precursor and Gonadoliberin I and GnRH-associated peptide I active peptides of human origin by WB, IP, IF, IHC(P) and ELISA
Anti-GnRH I Antibody (A-4) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of GnRH I (A-4): sc-271918.
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Ships via FedEx Ground to Contiguous US, Alaska, Canada, Monday through Friday. This method is used for less temperature sensitive items such as lab ware and animal
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Labware ships FedEx Ground free of charge to the contiguous US
GnRH I Antibody (A-4) is a high quality monoclonal GnRH I antibody (also designated GnRH I antibody) suitable for the detection of the GnRH I protein of human origin. GnRH I Antibody (A-4) is available as both the non-conjugated anti-GnRH I antibody form, as well as multiple conjugated forms of anti-GnRH I antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. Human reproduction is controlled by the hypothalamic-pituitary gonadal axis laid down ealry in fetal development. Luteinizing hormone releasing hormone (LHRH), also known as gonadotropin releasing hormone (GnRH), luliberin, gonadorelin or GnRH-associated peptide, is a decapeptide that is an important molecule in the hypothalamic-pituitary-gonadal axis control circuit. GnRH is produced by hypothalamic neurons and secreted in a pulsatile manner into the capillary plexus of the medianeminence. GnRH affects the release of lutenizing hormone and follicle stimulating hormone from gonadotropic cells in the anterior pituitary. In addition to hypothalamic GnRH (GnRH-I), a second GnRH form (GnRH-II) functions primarily in the midbrain. GnRH is expressed in the acrosomal region of human sperm, and in the anterior pituitary tissue and cancer cells. Unlike GnRH-I, GnRH-II is highly expressed outside the brain, particularly in the kidney, bone marrow and prostate, suggesting that it may have multiple functions. GnRH binds to a specific G protein-coupled receptor in the pituitary to regulate synthesis and secretion of gonadotropins. The genes encoding GnRH-I and -II map to human chromosome 8p21-p11.2 and 20p13, respectively.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
How can GnRH I (A-4): sc-271918 mouse monoclonal antibody be used for double or triple staining, if the other primary antibodies are also raised in mouse?
Asked by: Germaine
In this case, we recommend using a directly conjugated mouse monoclonal primary antibody. This antibody is available conjugated to HRP (sc-271918 HRP), phycoerythrin (sc-271918 PE), fluoroscein (sc-271918 FITC) and AlexaFluors.
Rated 5 out of
Good specificityGnRH I antibody is very clear in western bloting it is predictable and reliable. The experimental results are good.
Date published: 2017-10-17
Rated 4 out of
Primate retinaAntibody was tested under standard conditions (Long Y, Bordt AS, Liu WS, Davis EP, Lee SJ, Tseng L, Chuang AZ, Whitaker CM, Massey SC, Sherman MB, Marshak DW. Wide-field diffuse amacrine cells in the monkey retina contain immunoreactive Cocaine- and Amphetamine-Regulated Transcript (CART). Peptides. 2016 Oct;84:22-35). The isolated retina of a baboon was fixed for 60 minutes in 4% paraformaldehyde. Tissue was incubated for 9 days in a 1:1,000 solution of primary antibody with detergent and a blocking agent at 4 °C, washed, then incubated in an affinity-purified secondary antibody for 4 days at 4 °C, washed, and mounted in medium containing DAPI. No peptide blocking control was performed.
Results: Light punctate labeling and low background throughout the optic fiber layer, ganglion cell layer, inner plexiform layer, and inner nuclear layer (less).
Date published: 2017-06-01
Rated 5 out of
Exceptional immunoperoxidase cytoplasmicExceptional immunoperoxidase cytoplasmic staining in formalin fixed, paraffin-embedded human urinary bladder tissue. -SCBT QC
Date published: 2015-01-26
Rated 5 out of
Nice Western Blot data of human recombinantNice Western Blot data of human recombinant GnRH I fusion protein. -SCBT QC
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