GnRH I Antibody (A-4): sc-271918
- GnRH I Antibody (A-4) is a mouse monoclonal IgG2b κ GnRH I antibody, cited in 1 publications, provided at 200 µg/ml
- raised against amino acids 1-92 representing full length Progonadoliberin I of human origin
- GnRH I Antibody (A-4) is recommended for detection of Progonadoliberin I precursor and Gonadoliberin I and GnRH-associated peptide I active peptides of human origin by WB, IP, IF, IHC(P) and ELISA
- Anti-GnRH I Antibody (A-4) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG2b BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for GnRH I Antibody (A-4) for WB and IHC(P) applications. These reagents are now offered in bundles with GnRH I Antibody (A-4) (see ordering information below).
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GnRH I Antibody (A-4) is a mouse monoclonal IgG2b kappa light chain antibody that detects GnRH I protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). Anti-GnRH I antibody (A-4) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. GnRH I plays a crucial role in human reproduction through function in the hypothalamic-pituitary-gonadal axis, which is established early in fetal development. As a decapeptide, GnRH I is synthesized by hypothalamic neurons and released in a pulsatile manner into the capillary plexus of the median eminence, where GnRH I stimulates the secretion of luteinizing hormone and follicle-stimulating hormone from gonadotropic cells in the anterior pituitary. This regulation is vital for reproductive health, as GnRH I influences the development and function of the gonads. GnRH I is expressed in the acrosomal region of human sperm and in anterior pituitary tissue, as well as in certain cancer cells, indicating broader biological significance. A second form, GnRH II, primarily expressed outside the brain, suggests these peptides may have distinct and complementary roles in various physiological processes. The genes encoding GnRH-I and -II are located on human chromosomes 8p21-p11.2 and 20p13, respectively, highlighting their evolutionary importance.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Contains Mercury: All HRP conjugates (including bundle forms) contain thiomersal, a mercury compound. Do not dispose in trash. Dispose as hazardous chemical waste according to local, state and federal regulations.
Download thiomersal SDS
GnRH I Antibody (A-4) References:
- Influence of carrier protein conjugation site and terminal modification of a GnRH-I peptide sequence in the development of a highly specific anti-fertility vaccine. Part I. | Ferro, VA., et al. 2002. Am J Reprod Immunol. 48: 361-71. PMID: 12607772
- Use of a highly specific monoclonal antibody against the central variable amino acid sequence of mammalian gonadotropin releasing hormone to evaluate GnRH-I tissue distribution compared with GnRH-I binding sites in adult male rats. | Khan, MA., et al. 2003. Am J Reprod Immunol. 49: 239-48. PMID: 12852498
- Possible interactions between leptin, gonadotrophin-releasing hormone (GnRH-I and II) and human chorionic gonadotrophin (hCG). | Islami, D., et al. 2003. Eur J Obstet Gynecol Reprod Biol. 110: 169-75. PMID: 12969578
- Differential role of progesterone receptor isoforms in the transcriptional regulation of human gonadotropin-releasing hormone I (GnRH I) receptor, GnRH I, and GnRH II. | An, BS., et al. 2005. J Clin Endocrinol Metab. 90: 1106-13. PMID: 15562029
- Expression of gonadotropin-releasing hormone type-I (GnRH-I) and type-II (GnRH-II) in human peripheral blood mononuclear cells (PMBCs) and regulation of B-lymphoblastoid cell proliferation by GnRH-I and GnRH-II. | Tanriverdi, F., et al. 2004. Exp Clin Endocrinol Diabetes. 112: 587-94. PMID: 15578334
- Extracellular loop 3 (EL3) and EL3-proximal transmembrane helix 7 of the mammalian type I and type II gonadotropin-releasing hormone (GnRH) receptors determine differential ligand selectivity to GnRH-I and GnRH-II. | Li, JH., et al. 2005. Mol Pharmacol. 67: 1099-110. PMID: 15635044
- Development of novel 68Ga- and 18F-labeled GnRH-I analogues with high GnRHR-targeting efficiency. | Schottelius, M., et al. 2008. Bioconjug Chem. 19: 1256-68. PMID: 18510351
- Chitosan formulations improve the immunogenicity of a GnRH-I peptide-based vaccine. | Sáenz, L., et al. 2009. Int J Pharm. 369: 64-71. PMID: 19041932
- Agonists and antagonists of GnRH-I and -II reduce metastasis formation by triple-negative human breast cancer cells in vivo. | Schubert, A., et al. 2011. Breast Cancer Res Treat. 130: 783-90. PMID: 21279682
- Hindbrain lactate regulates preoptic gonadotropin-releasing hormone (GnRH) neuron GnRH-I protein but not AMPK responses to hypoglycemia in the steroid-primed ovariectomized female rat. | Shrestha, PK. and Briski, KP. 2015. Neuroscience. 298: 467-74. PMID: 25934033
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
GnRH I Antibody (A-4) | sc-271918 | 200 µg/ml | $322.00 | |||
GnRH I Antibody (A-4): m-IgG Fc BP-HRP Bundle | sc-529287 | 200 µg Ab; 10 µg BP | $361.00 | |||
GnRH I Antibody (A-4): m-IgGκ BP-HRP Bundle | sc-522101 | 200 µg Ab, 40 µg BP | $361.00 | |||
GnRH I Antibody (A-4): m-IgG2b BP-HRP Bundle | sc-550089 | 200 µg Ab; 10 µg BP | $361.00 | |||
GnRH I Antibody (A-4) AC | sc-271918 AC | 500 µg/ml, 25% agarose | $424.00 | |||
GnRH I Antibody (A-4) HRP | sc-271918 HRP | 200 µg/ml | $322.00 | |||
GnRH I Antibody (A-4) FITC | sc-271918 FITC | 200 µg/ml | $336.00 | |||
GnRH I Antibody (A-4) PE | sc-271918 PE | 200 µg/ml | $349.00 | |||
GnRH I Antibody (A-4) Alexa Fluor® 488 | sc-271918 AF488 | 200 µg/ml | $364.00 | |||
GnRH I Antibody (A-4) Alexa Fluor® 546 | sc-271918 AF546 | 200 µg/ml | $364.00 | |||
GnRH I Antibody (A-4) Alexa Fluor® 594 | sc-271918 AF594 | 200 µg/ml | $364.00 | |||
GnRH I Antibody (A-4) Alexa Fluor® 647 | sc-271918 AF647 | 200 µg/ml | $364.00 | |||
GnRH I Antibody (A-4) Alexa Fluor® 680 | sc-271918 AF680 | 200 µg/ml | $364.00 | |||
GnRH I Antibody (A-4) Alexa Fluor® 790 | sc-271918 AF790 | 200 µg/ml | $364.00 |