GnRH I Antibody (A-4) recommended for detection of Progonadoliberin I precursor and Gonadoliberin I and GnRH-associated peptide I active peptides of human origin by WB, IP, IF, IHC(P) and ELISA

GnRH I Antibody (A-4): sc-271918

GnRH I Antibody (A-4) is rated 4.8 out of 5 by 4.
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Datasheets

    • Anti-GnRH I Antibody (A-4) is a mouse monoclonal IgG2b (kappa light chain) GnRH I antibody provided at 200 µg/ml
    • raised against amino acids 1-92 representing full length Progonadoliberin I of human origin
    • GnRH I Antibody (A-4) is recommended for detection of Progonadoliberin I precursor and Gonadoliberin I and GnRH-associated peptide I active peptides of human origin by WB, IP, IF, IHC(P) and ELISA
    • Anti-GnRH I Antibody (A-4) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of GnRH I (A-4): sc-271918.

How can GnRH I (A-4): sc-271918 mouse monoclonal antibody be used for double or triple staining, if the other primary antibodies are also raised in mouse?

Asked by: Germaine
In this case, we recommend using a directly conjugated mouse monoclonal primary antibody. This antibody is available conjugated to HRP (sc-271918 HRP), phycoerythrin (sc-271918 PE), fluoroscein (sc-271918 FITC) and AlexaFluors.
Answered by: Technical Support
Date published: 2017-02-28
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Rated 5 out of 5 by from Good specificity GnRH I antibody is very clear in western bloting it is predictable and reliable. The experimental results are good.
Date published: 2017-10-17
Rated 4 out of 5 by from Primate retina Antibody was tested under standard conditions (Long Y, Bordt AS, Liu WS, Davis EP, Lee SJ, Tseng L, Chuang AZ, Whitaker CM, Massey SC, Sherman MB, Marshak DW. Wide-field diffuse amacrine cells in the monkey retina contain immunoreactive Cocaine- and Amphetamine-Regulated Transcript (CART). Peptides. 2016 Oct;84:22-35). The isolated retina of a baboon was fixed for 60 minutes in 4% paraformaldehyde. Tissue was incubated for 9 days in a 1:1,000 solution of primary antibody with detergent and a blocking agent at 4 °C, washed, then incubated in an affinity-purified secondary antibody for 4 days at 4 °C, washed, and mounted in medium containing DAPI. No peptide blocking control was performed. Results: Light punctate labeling and low background throughout the optic fiber layer, ganglion cell layer, inner plexiform layer, and inner nuclear layer (less).
Date published: 2017-06-01
Rated 5 out of 5 by from Exceptional immunoperoxidase cytoplasmic Exceptional immunoperoxidase cytoplasmic staining in formalin fixed, paraffin-embedded human urinary bladder tissue. -SCBT QC
Date published: 2015-01-26
Rated 5 out of 5 by from Nice Western Blot data of human recombinant Nice Western Blot data of human recombinant GnRH I fusion protein. -SCBT QC
Date published: 2013-10-09
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