GM3 Synthase Antibody (B-12): sc-365329
- GM3 Synthase Antibody (B-12) is a mouse monoclonal IgG2b κ GM3 Synthase antibody, cited in 3 publications, provided at 200 µg/ml
- raised against amino acids 11-135 mapping near the N-terminus of GM3 Synthase of human origin
- GM3 Synthase Antibody (B-12) is recommended for detection of GM3 Synthase of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-GM3 Synthase Antibody (B-12) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG2b BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for GM3 Synthase Antibody (B-12) for WB applications. These reagents are now offered in bundles with GM3 Synthase Antibody (B-12) (see ordering information below).
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GM3 Synthase Antibody (B-12) is a mouse monoclonal IgG2b kappa light chain antibody that detects GM3 Synthase of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-GM3 Synthase antibody (B-12) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. GM3 Synthase, also known as Sialyltransferase 9 or ST3Gal V, is a Golgi type II transmembrane glycosyltransferase predominantly expressed in the brain and placenta, playing a crucial role in the biosynthesis of complex gangliosides. This enzyme catalyzes the transfer of α-sialic acid to the terminal galactose of lactosylceramide, resulting in the formation of GM3, the simplest ganglioside. GM3 is essential for various cellular processes, including cell differentiation, signal transduction, and the modulation of cell proliferation. The synthesis of GM3 by GM3 Synthase represents the first major step in the formation of nearly all other gangliosides, highlighting GM3 Synthase′s importance as a key regulatory enzyme in ganglioside biosynthesis. Notably, mutations in the gene encoding GM3 Synthase can lead to severe neurological disorders, such as Amish infantile epilepsy syndrome, due to the inability to synthesize α- and β-series gangliosides.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
GM3 Synthase Antibody (B-12) References:
- Combinatorial PCR approach to homology-based cloning: cloning and expression of mouse and human GM3-synthase. | Kapitonov, D., et al. 1999. Glycoconj J. 16: 337-50. PMID: 10619706
- Evidence supporting a late Golgi location for lactosylceramide to ganglioside GM3 conversion. | Allende, ML., et al. 2000. Glycobiology. 10: 1025-32. PMID: 11030748
- Genomic structure of human GM3 synthase gene (hST3Gal V) and identification of mRNA isoforms in the 5'-untranslated region. | Kim, KW., et al. 2001. Gene. 273: 163-71. PMID: 11595162
- Isolation and characterization of the promoter region of the human GM3 synthase gene. | Kim, SW., et al. 2002. Biochim Biophys Acta. 1578: 84-9. PMID: 12393190
- Molecular mechanism for transcriptional activation of ganglioside GM3 synthase and its function in differentiation of HL-60 cells. | Chung, TW., et al. 2005. Glycobiology. 15: 233-44. PMID: 15385432
- Infantile-onset symptomatic epilepsy syndrome caused by a homozygous loss-of-function mutation of GM3 synthase. | Simpson, MA., et al. 2004. Nat Genet. 36: 1225-9. PMID: 15502825
- Human GM3 synthase: a new mRNA variant encodes an NH2-terminal extended form of the protein. | Berselli, P., et al. 2006. Biochim Biophys Acta. 1759: 348-58. PMID: 16934889
- Genetic diseases of sphingolipid metabolism: pathological mechanisms and therapeutic options. | Kacher, Y. and Futerman, AH. 2006. FEBS Lett. 580: 5510-7. PMID: 16970941
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
GM3 Synthase Antibody (B-12) | sc-365329 | 200 µg/ml | $322.00 | |||
GM3 Synthase Antibody (B-12): m-IgG Fc BP-HRP Bundle | sc-529351 | 200 µg Ab; 10 µg BP | $361.00 | |||
GM3 Synthase Antibody (B-12): m-IgGκ BP-HRP Bundle | sc-522209 | 200 µg Ab, 40 µg BP | $361.00 | |||
GM3 Synthase Antibody (B-12): m-IgG2b BP-HRP Bundle | sc-550095 | 200 µg Ab; 10 µg BP | $361.00 | |||
GM3 Synthase Antibody (B-12) AC | sc-365329 AC | 500 µg/ml, 25% agarose | $424.00 | |||
GM3 Synthase Antibody (B-12) HRP | sc-365329 HRP | 200 µg/ml | $322.00 | |||
GM3 Synthase Antibody (B-12) FITC | sc-365329 FITC | 200 µg/ml | $336.00 | |||
GM3 Synthase Antibody (B-12) PE | sc-365329 PE | 200 µg/ml | $349.00 | |||
GM3 Synthase Antibody (B-12) Alexa Fluor® 488 | sc-365329 AF488 | 200 µg/ml | $364.00 | |||
GM3 Synthase Antibody (B-12) Alexa Fluor® 546 | sc-365329 AF546 | 200 µg/ml | $364.00 | |||
GM3 Synthase Antibody (B-12) Alexa Fluor® 594 | sc-365329 AF594 | 200 µg/ml | $364.00 | |||
GM3 Synthase Antibody (B-12) Alexa Fluor® 647 | sc-365329 AF647 | 200 µg/ml | $364.00 | |||
GM3 Synthase Antibody (B-12) Alexa Fluor® 680 | sc-365329 AF680 | 200 µg/ml | $364.00 | |||
GM3 Synthase Antibody (B-12) Alexa Fluor® 790 | sc-365329 AF790 | 200 µg/ml | $364.00 |