GM-CSFRα Antibody (S-50): sc-456

GM-CSFRα Antibody (S-50) is a mouse monoclonal IgG1 kappa light chain antibody that detects the GM-CSFR alpha protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and flow cytometry (FCM). GM-CSFRα monoclonal antibody (S-50) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. The GM-CSFR alpha protein plays a crucial role in hematopoiesis and immune response by serving as a receptor for granulocyte-macrophage colony-stimulating factor (GM-CSF), which is essential for the proliferation, differentiation, and survival of myeloid cells. GM-CSFR alpha′s ability to mediate signaling pathways is vital for the regulation of immune responses, particularly in the context of inflammation and infection. The interaction of GM-CSFR alpha with its common β subunit enhances receptor affinity for GM-CSF, thereby amplifying the signaling cascade that leads to cellular responses. Understanding GM-CSFR alpha function is important for developing therapeutic strategies targeting immune-related diseases, as dysregulation of this pathway can contribute to various pathologies, including cancer and autoimmune disorders.

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GM-CSFRα Antibody (S-50) References:

1. Critical cytoplasmic domains of the common beta subunit of the human GM-CSF, IL-3 and IL-5 receptors for growth signal transduction and tyrosine phosphorylation.  |  Sakamaki, K., et al. 1992. EMBO J. 11: 3541-9. PMID: 1396555
2. Cloning of the low-affinity murine granulocyte-macrophage colony-stimulating factor receptor and reconstitution of a high-affinity receptor complex.  |  Park, LS., et al. 1992. Proc Natl Acad Sci U S A. 89: 4295-9. PMID: 1533931
3. Two distinct functional high affinity receptors for mouse interleukin-3 (IL-3).  |  Hara, T. and Miyajima, A. 1992. EMBO J. 11: 1875-84. PMID: 1582416
4. Cytokine receptors and signal transduction.  |  Miyajima, A., et al. 1992. Annu Rev Immunol. 10: 295-331. PMID: 1590989
5. Molecular cloning of a second subunit of the receptor for human granulocyte-macrophage colony-stimulating factor (GM-CSF): reconstitution of a high-affinity GM-CSF receptor.  |  Hayashida, K., et al. 1990. Proc Natl Acad Sci U S A. 87: 9655-9. PMID: 1702217
6. A human high affinity interleukin-5 receptor (IL5R) is composed of an IL5-specific alpha chain and a beta chain shared with the receptor for GM-CSF.  |  Tavernier, J., et al. 1991. Cell. 66: 1175-84. PMID: 1833065
7. Pulmonary alveolar proteinosis caused by deletion of the GM-CSFRalpha gene in the X chromosome pseudoautosomal region 1.  |  Martinez-Moczygemba, M., et al. 2008. J Exp Med. 205: 2711-6. PMID: 18955567
8. STAT3-activated GM-CSFRα translocates to the nucleus and protects CLL cells from apoptosis.  |  Li, P., et al. 2014. Mol Cancer Res. 12: 1267-82. PMID: 24836891
9. The VICM biomarker is released from activated macrophages and inhibited by anti-GM-CSFRα-mAb treatment in rheumatoid arthritis patients.  |  Mortensen, JH., et al. 2019. Clin Exp Rheumatol. 37: 73-80. PMID: 30418117
10. Dense GM-CSFRα-expressing immune infiltration is allied with longer survival of intrahepatic cholangiocarcinoma patients.  |  Saranaruk, P., et al. 2023. PeerJ. 11: e14883. PMID: 36883059
11. Human IL-3 receptor signaling: rapid induction of phosphatidylcholine hydrolysis is independent of protein kinase C but dependent on tyrosine phosphorylation in transfected NIH 3T3 cells.  |  Rao, P., et al. 1995. J Immunol. 154: 1664-74. PMID: 7836750
12. A model for the interaction of the GM-CSF, IL-3 and IL-5 receptors with their ligands.  |  Goodall, GJ., et al. 1993. Growth Factors. 8: 87-97. PMID: 8466757

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-GM-CSFRα Antibody (S-50) has 11 citations in a variety of scientific publications.

Primary antibodies like Anti-GM-CSFRα Antibody (S-50) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

GM-CSFRα Antibody (S-50) Specifications

Alternate Product Name: CD116

Product Clone Name: S-50

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids:

Epitope Target: GM-CSFRα

Epitope Species: human

Product Reactivity/Detection: GM-CSFRα

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, FCM

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 11 citations in various scientific publications

Available Conjugates/Forms for GM-CSFRα Antibody (S-50): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin