GlyRS Antibody (F-5): sc-365442
- GlyRS Antibody (F-5) is a mouse monoclonal IgG2a κ, cited in 1 publications, provided at 200 µg/ml
- specific for an epitope mapping between amino acids 136-165 within an internal region of GlyRS of human origin
- recommended for detection of GlyRS of mouse, rat, human and avian origin by WB, IP, IF and ELISA; also reactive with additional species, including and equine, canine, bovine, porcine and avian
- TransCruz reagent for ChIP application (sc-365442 X, 200 µg/0.1 ml)
- m-IgG Fc BP-HRP, m-IgG2a BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for GlyRS Antibody (F-5) for WB applications. These reagents are now offered in bundles with GlyRS Antibody (F-5) (see ordering information below).
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GlyRS Antibody (F-5) is a mouse monoclonal IgG2a antibody that detects GlyRS in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). GlyRS, or Glycyl-tRNA synthetase, is a crucial enzyme composed of 739 amino acids that plays a vital role in protein synthesis by catalyzing the attachment of glycine to its corresponding tRNA, thereby ensuring accurate translation of genetic information into functional proteins. GlyRS is predominantly expressed in the brain and spinal cord, highlighting GlyRS importance in neural function and development. Notably, defects in GlyRS are linked to Charcot-Marie-Tooth disease type 2D (CMT2D), an autosomal dominant disorder characterized by significant muscle weakness and atrophy, particularly affecting the upper extremities. Additionally, mutations in GlyRS can lead to distal hereditary muscular neuropathy type V (HMN5), which presents with similar symptoms but typically with less severe sensory involvement. Anti-GlyRS antibody (F-5) is an essential tool for researchers studying these conditions, enabling investigation of GlyRS expression and function in various biological contexts.
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GlyRS Antibody (F-5) References:
- Glycyl-tRNA synthetase specifically binds to the poliovirus IRES to activate translation initiation. | Andreev, DE., et al. 2012. Nucleic Acids Res. 40: 5602-14. PMID: 22373920
- Impaired function is a common feature of neuropathy-associated glycyl-tRNA synthetase mutations. | Griffin, LB., et al. 2014. Hum Mutat. 35: 1363-71. PMID: 25168514
- Crystal Structure of the Wild-Type Human GlyRS Bound with tRNA(Gly) in a Productive Conformation. | Qin, X., et al. 2016. J Mol Biol. 428: 3603-14. PMID: 27261259
- Aberrant GlyRS-HDAC6 interaction linked to axonal transport deficits in Charcot-Marie-Tooth neuropathy. | Mo, Z., et al. 2018. Nat Commun. 9: 1007. PMID: 29520015
- GlyRS is a new mediator of amino acid-induced milk synthesis in bovine mammary epithelial cells. | Luo, C., et al. 2019. J Cell Physiol. 234: 2973-2983. PMID: 30171693
- Amino acids stimulate glycyl-tRNA synthetase nuclear localization for mammalian target of rapamycin expression in bovine mammary epithelial cells. | Yu, M., et al. 2019. J Cell Physiol. 234: 7608-7621. PMID: 30471104
- Methionine stimulates GlyRS phosphorylation via the GPR87-CDC42/Rac1-MAP3K10 signaling pathway. | Luo, C., et al. 2020. Biochem Biophys Res Commun. 523: 847-852. PMID: 31954518
- Extracellular vesicles derived from macrophages display glycyl-tRNA synthetase 1 and exhibit anti-cancer activity. | Goughnour, PC., et al. 2020. J Extracell Vesicles. 10: e12029. PMID: 33708357
- Charcot-Marie-Tooth mutation in glycyl-tRNA synthetase stalls ribosomes in a pre-accommodation state and activates integrated stress response. | Mendonsa, S., et al. 2021. Nucleic Acids Res. 49: 10007-10017. PMID: 34403468
- Two distinct receptor-binding domains of human glycyl-tRNA synthetase 1 displayed on extracellular vesicles activate M1 polarization and phagocytic bridging of macrophages to cancer cells. | Park, MC., et al. 2022. Cancer Lett. 539: 215698. PMID: 35523311
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
GlyRS Antibody (F-5) | sc-365442 | 200 µg/ml | $322.00 | |||
GlyRS Antibody (F-5): m-IgG Fc BP-HRP Bundle | sc-537802 | 200 µg Ab; 10 µg BP | $361.00 | |||
GlyRS Antibody (F-5): m-IgGκ BP-HRP Bundle | sc-535198 | 200 µg Ab; 40 µg BP | $361.00 | |||
GlyRS Antibody (F-5): m-IgG2a BP-HRP Bundle | sc-548124 | 200 µg Ab; 10 µg BP | $361.00 | |||
GlyRS (F-5) Neutralizing Peptide | sc-365442 P | 100 µg/0.5 ml | $69.00 | |||
GlyRS Antibody (F-5) X | sc-365442 X | 200 µg/0.1 ml | $322.00 |